PSMF1_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PSMF1_DROME
• UniProt AC: Q9V637
• Protein Name: Proteasome inhibitor PI31 subunit
• Gene Name: PI31
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 270
• Subcellular Localization: Cytoplasm . In the spermatid, localizes to the actin-based individualization complex.
• Protein Description: Plays an important role in the control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Enhances 26S proteasome function by promoting its assembly through the interaction with the assembly chaperones PSMD9 and PMSD5. Functions together with ntc to control non-apoptotic caspase activation during sperm individualization. In testis, is required for proper protein degradation and germline cell cycle progression..
• Protein Sequence: MESGSTAKTGDFFYGWDLLYKTVKADVSKKSDLLIALVHFLLTKHYNFRCVGVGDDKTLPEEEGSELLPDSWNDDDTKYSLRYVHDKMLYLLLGHITEGSLLINLLDINTKKVSNICVEPETLVPEVKGGITTIMPSASEIVERYRRELLDPVFTGNSREVTTQTTNSPRPIGSDPDPLRIGEPRRGGSFIPSAFEPRPFGFPDVGRGDLDPLGRGGHGNLFSFPSRPNMGPGPVPRFDPFNPLNPNRPGQGGINPDHMRPPDWNPDYYM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
162	Phosphorylation	TGNSREVTTQTTNSP CCCCCEEEEECCCCC	14.40	23607784
163	Phosphorylation	GNSREVTTQTTNSPR CCCCEEEEECCCCCC	29.15	23607784
165	Phosphorylation	SREVTTQTTNSPRPI CCEEEEECCCCCCCC	26.33	29892262
166	Phosphorylation	REVTTQTTNSPRPIG CEEEEECCCCCCCCC	24.71	28490779
168	Phosphorylation	VTTQTTNSPRPIGSD EEEECCCCCCCCCCC	21.86	23607784
189	Phosphorylation	GEPRRGGSFIPSAFE CCCCCCCCCCCCCCC	23.87	19429919
223	Phosphorylation	GGHGNLFSFPSRPNM CCCCCCCCCCCCCCC	39.14	21082442
226	Phosphorylation	GNLFSFPSRPNMGPG CCCCCCCCCCCCCCC	59.36	21082442

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PSMF1_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PSMF1_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PSMF1_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TNKS_DROME	Tnks	physical	23622245
PSMD5_DROME	CG12096	physical	23622245
PSMD9_DROME	CG9588	physical	23622245

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of Drosophila melanogaster embryos.";
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
J. Proteome Res. 7:1675-1682(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, AND MASSSPECTROMETRY.
PubMed: 18327897

"An integrated chemical, mass spectrometric and computational strategyfor (quantitative) phosphoproteomics: application to Drosophilamelanogaster Kc167 cells.";
Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D.,Juenger M.A., Eng J.K., Aebersold R., Tao W.A.;
Mol. Biosyst. 3:275-286(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-189, ANDMASS SPECTROMETRY.
PubMed: 17372656