PRKDC_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PRKDC_MOUSE
• UniProt AC: P97313
• Protein Name: DNA-dependent protein kinase catalytic subunit
• Gene Name: Prkdc
• Organism: Mus musculus (Mouse).
• Sequence Length: 4128
• Subcellular Localization: Nucleus . Nucleus, nucleolus .
• Protein Description: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, C1D, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (By similarity)..
• Protein Sequence: MAEEGTGVRCWLLQLQEFLSAADRCSAAGASYQLIRSLGQECVLSTSSAVQALQISLVFSRDFGLLVFIRKSLSIEDFRDCREEALKFLCVFLEKIDQKVMHYSLDIKNTCTSVYTKDRTAKCKIPALDLLIKLLQILRSTRLMDEFKIGELFNKFYGELASKSKLPDTVLEKVYELLGVLGEVHPSEMINHSENLFRAFLGELKTQMTSTVREPKFPVLAGCLKGLSSLLCNFTKSMEEDPQTSKEIFGFTFKAIRPQIEMKRYAVPLAGLRLLTLHASQFTACLLDNYITLFEVLSKWCSHTNVELKKAAHSALESFLRQISFTVAEDAELHKSRLKYFMEQFYGIIRNTDSNNKELAIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTHADASEDHVYQMPSFLQSIASVLLYLDTVPEVYTPVLEHLMVVQIDSFPQYSPKMQLVCCKAIIKLFLALSEKGPVHWNCISAVVHQGLIRICSKPVVLQKDVESRSDNRSASEEVRTGRWKVPTYKDYVDLFQHLLGCDQMEDFILGDETFLFVNSSLKSLNHLLYDEFIRSVLKIVEKLDLTLEKQTVGEQEDGSTADVWVIPTSDPAANLHPAKPSDFSALINLVEFCREILPRKHVGFFEPWVYSFAYELILQSTRLPLISGFYKLLSIAVKNARKIKYFEGISPKSLKHSPEDTEKYSCFALFAKFGKEVSVKMKQYKDELLASCLTFVLSLPHDIIELDVRAYVPALQMAFKLGLSHMPLAEIGLHALKEWSVHIDKSILQPYYKDILPCLDGYLNTSTLSDETKSHWGLSALSRAAQKGFNRHVVKHLKRTRNSSPDEALSLEEIRIKVVQILGSLGGQINKSLVTATSGERMKKYVAWDAERRLSFAVPFREMKPVIYLDVFLPRVTELALSASDRQTKVAACELLHSMVMFMLGRATQMPEGQGLPPMYQLYKHTFPVLLQLACDVDQVTRQLYEPLVMQLIHWLTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCVQEFLKWSIKQTTPQQQEKSPVNSKSLFKRLYSLALHPNAFKRLGAALAFNHIYKEFREEGSLVEQFVFEALVTYMESLALAHEDEKSLGTVQQCCDAIDHLRRIIEKKHVSLNKAKKRRLPQGFPPLTSLCLLDLVEWLLAHCGRPQTECRHKSMELFYKFVPLLPGNKSPSLWLKDLIKKKGISFLINTFEGGASSSDQPAGILAQPTLVYLQGPISLRGVLQWLDLLLAALECYNTFIEKETVQGQEVLGAEVQSSLLKSVAFFLESIATHSARAVEQRFGSGAPGPPSLHEEEKYNYSKCTVLVRIMEFTTTLLIASPEDCKLLEKDLCNTNLMQVLVKMICEPMSLGFNIGDVQVMNHLPSICVNLLKALRKSPYRDMLETHLKEKVTVQSVEELCSINLCSSGARQERSKLLSILSACKQLHKAGFSHVISPSQSTALNHSVGMRLLSLVYKGIVPAEERQCLQSLDPSCKSLANGLLELAFGFGGLCDHLVSLLLNSAMLSTQYLGSSQRNISFSHGEYFYSLFSEVINSELLKNLDIAVSRLMESSSDNPKMVSTVLNGMLDTSFRDRAVQKHQGLKLATAILQNWRKCDSWWAPDSAPESKTTVLSLLAKMLQIDSALSFDTNHSSFSEIFTTYASLLADTKLGLHLKGQAIILLPFFTSLREGSLENLKHILEKLIVCNFPMKSDEFPPDSLKYNNYVDCMKKFLDALELSQSPMLFQLMTDILCREQRHIMEELFQTTFKRIARQSPCVTQLNLLESVYTMFRKADLPSNVTRQAFVDRSLLTLLWHCDLDTLKEFFSRIVVDAIDVLKSRFTKLNEFTFDTQITKKMCYYKMLAVMYSRLLKDDVHSKEAKINQAFHGSRVAEGNELTKTLLKLCHDAFTENMVGESQLLEKRRLYHCAAYNCAISLISCVFNELKFYQGFLFNEKPEKNLFIFENLIDLKRCYTFPIEVEVPMERKKKYIEIRKEARDAANGASGSPHYMSSLSYLTDSSLSEEMSQFDFSTGVQSYSYSSQDRKPTTGHFQRREHQDSMTQDDIMELEMDELNQHECMAPMIALIKHMQRNVIAPKGEEGSIPKDLPPWMKFLHDKLGNASVSLNIRLFLAKLVINTEEVFRPYAKHWLSPLLQLAVCENNREGIHYMMVEIVATILSWTGLATPTGVPKDEVLANRLLRFLMKHVFHPKRAVFRHNLEIIKTLVECWKECLSIPYRLIFEKFSHKDPNSKDNSVGIQLLGIVIANNLPPYDPNCDITSAMYFEALVNNMSFVKYKEVYAAAAEVLGLILQYITERKHVIAELVCELVIKQLKQHQNTMEDKFIVCLNKIAKGFPPLADRFLNALFFLLPKFHGVMKTLCLEVVLCRAEEITGLYLQLKSKDFLQVMRHRDDERQKVCLDIVYKMVAKLKPIELRELLNPVVEFVSHPSPTCREQMYNILMWIHDNYRDQESQNDEDSQEIFKLAKDVLIQGLIDENVGLQLIIRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTRMSPDYLNPIFEHPLSECEFQEYTIDPDWRFRSTVLTPMFIETQASPSILHTQTQEGPLSDQRQKPGQVRATQQQYDFTPTQASVERSSFDWLTGSSIDLLADHTVFSSETLSSSLLFSHKRTEKSQRMSCKSVGPDFGTKKLGLPDDEVDNQVKSGTPSQADILRLRRRFLKDREKLSLLYAKRGLMEQKLEKDIKSEFKMKQDAQVVLYRSYRHGDLPDIQIQHSGLITPLQAVAQKDPIIAKQLFSSLFSGILKEMNKFKTTSEKNIITQNLLQDFNRFLNTTFLFFPPFVSCIQEISCQHPDFLTLDPASVRVGCLASLQQPGGIRLLEEALLRLMPKEPPTKRVRGKTCLPPDVLRWMELAKLYRSIGEYDVLRGIFSSELGTTQDTQNALLAEARSDYCQAAKLYDEALNKLEWVDGEPTEAEKEFWELASLDCYNNLSKWKELEYCSTVNIVSENSLDLSKMWSEPFYQETYLPYVIRSKLKLLLQGEGNQSLLTFVDEAMNKELQKTVLELQYSQELSLLYILQDDIDRATYYIKNGIQIFMQNYSSIDVLLYRSRLAKLQSVQTLAEIEEFLSFICKHGDLSSLGPLRRLLKTWTSRYPDVVTDPMHIWDDIITNRCFFLSKIEERLTAPSGDHSMSVDEDEESIDREVYEPKEDVRCMLQSCRFTMKMKMIESAWKQSNFSLSMKLLKEMHKESKTREIWRVQWLHSYSQLNHCRSHTQSPREQVLNMLKTITLLDESDISNYLNKNIQASCDQSILLGTTCRIMADALSREPACLSDLEENKVNSILTLSGSNAENTETVITGLYQRAFHHLSKAVQSAEEETQLSCWGHEAAAERAHAYMTLVGFCDQQLRKVEESASQKTSAEMEAYPALVVEKMLRALKLNSSEARLKFPRLLQIIEQYSEETLNIMTKEISSIPCWQFIGWISHMMALLDKEEAIAVQHTVEEIADNYPQAIIYPFIISSESYSFKNTSSGHNNKAFVERIKSKLDHGEVIHSFINALDQLSNPDLLFKDWVSDTKDELGKNPVNKKNIEKLYERMYAALGDLRAPGLGPFRRRFIQAFGKEFVKSFGNGGSKLLTMKVDDFCKITGSLLVRMKKDSKLPGNLKEYSPWMSEFKAQFLKNELEIPGQYDGKSKPLPEYHVRISGFDERVKVMLSLRKPKRIVIRGHDEKEYPFLVKGGEDLRQDQRIEQIFEVMNAILSQDAACSQRNMQLRTYRVVPMTSRLGLIEWIENTMTLKDLLLSNMSQEEKVANNSDPKAPIRDYKDWLMKVSGKSDAGAYVLMYSRANRTETVVAFRRRESQVPPDLLKRAFVKMSTSPEAFLALRSHFASSHALLCISHWLLGIGDRHLNNFMVAMETGSVIGIDFGHAFGSATQFLPVPELMPFRLTRQFVSLMLPMKETGLMCTVMVHALRAFRSCAGLLTDTMEIFVKEPSFDWKSFEQTMLRKGGSWIQEINVTEKNWYPQHKIRYAKRKLAGANPAVITCDELYLGHEASSAFRSYTAVARGNRDYNIRAQEPESGLSEETQVKCLVDQATDPNILGRTWEGWEPWM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
117	Acetylation	TCTSVYTKDRTAKCK CCEEEEECCCCCCCC	27.76	-
366	Phosphorylation	LAIAIRGYGLFAGPC EEEEEECCEECCCCE	10.78	22817900
385	Phosphorylation	AKDVDFMYVELIQRC CCCCCCHHHHHHHHH	7.26	-
511	Phosphorylation	RSDNRSASEEVRTGR CCCCCCCCHHHHCCC	35.22	-
646	Phosphorylation	GFFEPWVYSFAYELI CCCCHHHHHHHHHHH	8.33	-
686	Phosphorylation	IKYFEGISPKSLKHS CCEECCCCHHHCCCC	36.81	-
714	Phosphorylation	AKFGKEVSVKMKQYK HHHCCCCCHHHHHHH	19.67	-
839	Phosphorylation	HLKRTRNSSPDEALS HHHHHCCCCHHHCCC	39.70	28066266
840	Phosphorylation	LKRTRNSSPDEALSL HHHHCCCCHHHCCCH	39.66	28066266
846	Phosphorylation	SSPDEALSLEEIRIK CCHHHCCCHHHHHHH	40.56	30635358
881	Phosphorylation	SGERMKKYVAWDAER CCCHHHHHHHHCCCH	7.00	-
891	Phosphorylation	WDAERRLSFAVPFRE HCCCHHHHCEECHHH	14.89	22817900
1054	Malonylation	EKSPVNSKSLFKRLY CCCCCCHHHHHHHHH	46.07	25418362
1055	Phosphorylation	KSPVNSKSLFKRLYS CCCCCHHHHHHHHHH	38.57	21743459
1062	Phosphorylation	SLFKRLYSLALHPNA HHHHHHHHHHHCHHH	16.26	-
1141	Phosphorylation	IIEKKHVSLNKAKKR HHHHHCCCCCHHHHC	26.41	30482847
1206	Acetylation	KSPSLWLKDLIKKKG CCHHHHHHHHHHHCC	38.62	-
1577	Phosphorylation	KNLDIAVSRLMESSS HCHHHHHHHHHHCCC	15.63	29176673
1641	Phosphorylation	SAPESKTTVLSLLAK CCCCCHHHHHHHHHH	24.16	29109428
1809	Phosphorylation	FRKADLPSNVTRQAF HHHCCCCCCHHHHHH	51.75	28576409
1967	Acetylation	QGFLFNEKPEKNLFI CCCCCCCCCCCCEEE	60.63	-
2000	Acetylation	VPMERKKKYIEIRKE ECHHHHHHHHHHHHH	55.27	23576753
2053	Phosphorylation	VQSYSYSSQDRKPTT CCEECCCCCCCCCCC	28.06	-
2255	Acetylation	PYRLIFEKFSHKDPN CHHHHHHHHCCCCCC	41.22	-
2485	Phosphorylation	DNYRDQESQNDEDSQ HHCCCCCCCCCHHHH	28.75	22817900
2531	Phosphorylation	ETRLPSNTLDRLLAL CCCCCCCHHHHHHHH	34.08	-
2545	Ubiquitination	LNSLYSPKIEVHFLS HHHCCCCCCCHHHHH	45.63	-
2605	Phosphorylation	LTPMFIETQASPSIL ECCCEEECCCCCCCC	25.31	-
2608	Phosphorylation	MFIETQASPSILHTQ CEEECCCCCCCCCCC	14.90	-
2634	Phosphorylation	KPGQVRATQQQYDFT CCCCEEECCHHCCCC	19.15	-
2643	Phosphorylation	QQYDFTPTQASVERS HHCCCCCCCCCCCHH	33.44	-
2683	Acetylation	SSLLFSHKRTEKSQR HHHHHCCCCCHHHHC	60.87	30583541
2718	Phosphorylation	EVDNQVKSGTPSQAD HHHHHHHCCCCCHHH	49.39	25619855
2720	Phosphorylation	DNQVKSGTPSQADIL HHHHHCCCCCHHHHH	27.19	25619855
2722	Phosphorylation	QVKSGTPSQADILRL HHHCCCCCHHHHHHH	37.53	25619855
2746	Ubiquitination	KLSLLYAKRGLMEQK HHHHHHHHHCHHHHH	33.18	22790023
2746	Acetylation	KLSLLYAKRGLMEQK HHHHHHHHHCHHHHH	33.18	23576753
2945	Phosphorylation	DVLRGIFSSELGTTQ HHHHHHHHCCCCCCH	22.45	30635358
2946	Phosphorylation	VLRGIFSSELGTTQD HHHHHHHCCCCCCHH	26.54	30635358
2950	Phosphorylation	IFSSELGTTQDTQNA HHHCCCCCCHHHHHH	33.63	30635358
2951	Phosphorylation	FSSELGTTQDTQNAL HHCCCCCCHHHHHHH	23.73	30635358
2954	Phosphorylation	ELGTTQDTQNALLAE CCCCCHHHHHHHHHH	17.57	30635358
2971	Acetylation	SDYCQAAKLYDEALN HHHHHHHHHHHHHHH	51.07	23806337
2971	Malonylation	SDYCQAAKLYDEALN HHHHHHHHHHHHHHH	51.07	26320211
3206	Phosphorylation	TAPSGDHSMSVDEDE CCCCCCCCCCCCCCH	19.93	26370283
3208	Phosphorylation	PSGDHSMSVDEDEES CCCCCCCCCCCCHHH	29.45	26370283
3215	Phosphorylation	SVDEDEESIDREVYE CCCCCHHHHCHHHCC	27.95	25338131
3241	Acetylation	CRFTMKMKMIESAWK HHHHHHHHHHHHHHH	31.07	-
3250	Phosphorylation	IESAWKQSNFSLSMK HHHHHHHCCHHHHHH	35.79	23737553
3253	Phosphorylation	AWKQSNFSLSMKLLK HHHHCCHHHHHHHHH	24.64	23737553
3255	Phosphorylation	KQSNFSLSMKLLKEM HHCCHHHHHHHHHHH	16.81	23737553
3260	Acetylation	SLSMKLLKEMHKESK HHHHHHHHHHHHHHC	64.53	23576753
3288	Phosphorylation	SQLNHCRSHTQSPRE HHHHHHHHCCCCHHH	35.96	28285833
3290	Phosphorylation	LNHCRSHTQSPREQV HHHHHHCCCCHHHHH	31.80	28285833
3310	Phosphorylation	TITLLDESDISNYLN HHHCCCHHHHHHHHC	39.65	22817900
3313	Phosphorylation	LLDESDISNYLNKNI CCCHHHHHHHHCCCH	25.14	22817900
3432	Phosphorylation	RKVEESASQKTSAEM HHHHHHHHHCCCHHH	41.30	27841257
3545	Phosphorylation	ESYSFKNTSSGHNNK CCCCCCCCCCCCCCH	25.51	22817900
3546	Phosphorylation	SYSFKNTSSGHNNKA CCCCCCCCCCCCCHH	43.65	22817900
3593	Acetylation	KDWVSDTKDELGKNP CHHHCCCHHHHCCCC	54.95	23806337
3638	Acetylation	RFIQAFGKEFVKSFG HHHHHHHHHHHHHHC	41.34	-
3642	Acetylation	AFGKEFVKSFGNGGS HHHHHHHHHHCCCCC	45.44	-
3731	Phosphorylation	ERVKVMLSLRKPKRI HHHHHHHHCCCCCEE	14.18	-
3821	Phosphorylation	DLLLSNMSQEEKVAN HHHHCCCCHHHHHHC	38.40	-
3850	Phosphorylation	LMKVSGKSDAGAYVL HHHHCCCCCCCEEEE	35.90	21189417
4026	Phosphorylation	TMLRKGGSWIQEINV HHHHHCCCCEEEEEC	29.67	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PRKDC_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
2053	S	Phosphorylation	Autophosphorylated on Ser-2053, Thr-2605, Thr-2634 and Thr-2643.	32103174
2053	S	Phosphorylation	Ser-2053 and Thr-2605 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C (By similarity).	32103174
2605	T	Phosphorylation	Autophosphorylated on Ser-2053, Thr-2605, Thr-2634 and Thr-2643.	32103174
2605	T	Phosphorylation	Ser-2053 and Thr-2605 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C (By similarity).	32103174
2634	T	Phosphorylation	Autophosphorylated on Ser-2053, Thr-2605, Thr-2634 and Thr-2643.	32103174
2643	T	Phosphorylation	Autophosphorylated on Ser-2053, Thr-2605, Thr-2634 and Thr-2643.	32103174

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PRKDC_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
P53_MOUSE	Trp53	physical	15792956
TERA_MOUSE	Vcp	physical	23722536

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.