PPR35_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PPR35_HUMAN
• UniProt AC: Q8TAP8
• Protein Name: Protein phosphatase 1 regulatory subunit 35
• Gene Name: PPP1R35
• Organism: Homo sapiens (Human).
• Sequence Length: 253
• Protein Description: Inhibits PPP1CA phosphatase activity..
• Protein Sequence: MMMGCGESELKSADGEEAAAVPGPPPEPQVPQLRAPVPEPGLDLSLSPRPDSPQPRHGSPGRRKGRAERRGAARQRRQVRFRLTPPSPVRSEPQPAVPQELEMPVLKSSLALGLELRAAAGSHFDAAKAVEEQLRKSFQIRCGLEESVSEGLNVPRSKRLFRDLVSLQVPEEQVLNAALREKLALLPPQARAPHPKEPPGPGPDMTILCDPETLFYESPHLTLDGLPPLRLQLRPRPSEDTFLMHRTLRRWEA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
12	Phosphorylation	CGESELKSADGEEAA CCHHHCCCCCCCHHC	44.19	30266825
45	Phosphorylation	PEPGLDLSLSPRPDS CCCCCCCCCCCCCCC	26.85	29255136
47	Phosphorylation	PGLDLSLSPRPDSPQ CCCCCCCCCCCCCCC	18.58	29255136
52	Phosphorylation	SLSPRPDSPQPRHGS CCCCCCCCCCCCCCC	28.28	29255136
59	Phosphorylation	SPQPRHGSPGRRKGR CCCCCCCCCCCHHCH	20.32	26329039
84	Phosphorylation	RQVRFRLTPPSPVRS HEEEEEECCCCCCCC	28.97	22167270
87	Phosphorylation	RFRLTPPSPVRSEPQ EEEECCCCCCCCCCC	36.91	23401153
91	Phosphorylation	TPPSPVRSEPQPAVP CCCCCCCCCCCCCCC	54.59	22617229
128	Ubiquitination	GSHFDAAKAVEEQLR CCCHHHHHHHHHHHH	55.47	29967540
137	Phosphorylation	VEEQLRKSFQIRCGL HHHHHHHHHCHHCCC	18.89	23828894
147	Phosphorylation	IRCGLEESVSEGLNV HHCCCCHHHHCCCCC	22.90	23828894
149	Phosphorylation	CGLEESVSEGLNVPR CCCCHHHHCCCCCCH	34.91	23828894
157	Phosphorylation	EGLNVPRSKRLFRDL CCCCCCHHHHHHHHH	18.96	23828894
182	Ubiquitination	LNAALREKLALLPPQ HHHHHHHHHCCCCCC	32.40	-
216	Phosphorylation	CDPETLFYESPHLTL ECHHHCEECCCCCCC	20.71	27642862
241	Phosphorylation	RPRPSEDTFLMHRTL CCCCCCCCCHHHHHH	18.02	24719451
247	Phosphorylation	DTFLMHRTLRRWEA- CCCHHHHHHHHHCC-	15.22	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PPR35_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PPR35_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PPR35_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EDC4_HUMAN	EDC4	physical	26496610
PR40A_HUMAN	PRPF40A	physical	26496610
DCP1A_HUMAN	DCP1A	physical	26496610
EDC3_HUMAN	EDC3	physical	26496610
DCP2_HUMAN	DCP2	physical	26496610

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47 AND SER-52, AND MASSSPECTROMETRY.
PubMed: 19690332

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52, AND MASSSPECTROMETRY.
PubMed: 19369195

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-47 AND SER-52,AND MASS SPECTROMETRY.
PubMed: 19413330

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-47; SER-52;THR-84 AND SER-87, AND MASS SPECTROMETRY.
PubMed: 18669648

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND SER-52, AND MASSSPECTROMETRY.
PubMed: 18691976

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis.";
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III;
J. Proteome Res. 7:1346-1351(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-47 AND SER-52,AND MASS SPECTROMETRY.
PubMed: 18220336