PKHM1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PKHM1_HUMAN
• UniProt AC: Q9Y4G2
• Protein Name: Pleckstrin homology domain-containing family M member 1
• Gene Name: PLEKHM1
• Organism: Homo sapiens (Human).
• Sequence Length: 1056
• Subcellular Localization: Cytoplasm . Endosome membrane . Lysosome . Lysosome membrane . Localizes to the external membrane of autolysosomes (PubMed:25498145). In case of infection colocalizes with Salmonella typhimurium sifA in proximity of Salmonella-containing vacuole (SCV
• Protein Description: Proposed to act as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Seems to be involved in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion. [PubMed: 25498145 Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7]
• Protein Sequence: MLSVVENGLDPQAAIPVIKKKLVGSVKALQKQYVSLDTVVTSEDGDANTMCSALEAVFIHGLHAKHIRAEAGGKRKKSAHQKPLPQPVFWPLLKAVTHKHIISELEHLTFVNTDVGRCRAWLRLALNDGLMECYLKLLLQEQARLHEYYQPTALLRDAEEGEFLLSFLQGLTSLSFELSYKSAILNEWTLTPLALSGLCPLSELDPLSTSGAELQRKESLDSISHSSGSEDIEVHHSGHKIRRNQKLTASSLSLDTASSSQLSCSLNSDSCLLQENGSKSPDHCEEPMSCDSDLGTANAEDSDRSLQEVLLEFSKAQVNSVPTNGLSQETEIPTPQASLSLHGLNTSTYLHCEAPAEPLPAQAASGTQDGVHVQEPRPQAPSPLDLQQPVESTSGQQPSSTVSETAREVGQGNGLQKAQAHDGAGLKLVVSSPTSPKNKSWISEDDFYRPSREQPLESASDHPIASYRGTPGSRPGLHRHFSQEPRKNCSLGALDQACVPSPGRRQAQAAPSQGHKSFRVVHRRQMGLSNPFRGLMKLGTVERRGAMGIWKELFCELSPLEFRLYLSNEEHTCVENCSLLRCESVGPAHSDGRFELVFSGKKLALRASSQDEAEDWLDRVREALQKVRPQQEDEWVNVQYPDQPEEPPEAPQGCLSPSDLLSEPAALQGTQFDWSSAQVPEPDAIKESLLYLYMDRTWMPYIFSLSLEALKCFRIRNNEKMLSDSHGVETIRDILPDTSLGGPSFFKIITAKAVLKLQAGNAEEAALWRDLVRKVLASYLETAEEAVTLGGSLDENCQEVLKFATRENGFLLQYLVAIPMEKGLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYCDICHQDDASVIPARIIHNWDLTKRPICRQALKFLTQIRAQPLINLQMVNASLYEHVERMHLIGRRREQLKLLGDYLGLCRSGALKELSKRLNHRNYLLESPHRFSVADLQQIADGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICQHHDIIFPFEFDTTVRCAECKTVFHQSCQAVVKKGCPRCARRRKYQEQNIFA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MLSVVENGLD -----CCCHHHCCCC	35.66	20068231
25	Phosphorylation	IKKKLVGSVKALQKQ HHHHHHHCHHHHHHC	16.99	23911959
217	Ubiquitination	SGAELQRKESLDSIS CCHHHHCHHHHHHCC	38.40	29967540
219	Phosphorylation	AELQRKESLDSISHS HHHHCHHHHHHCCCC	40.28	23312004
222	Phosphorylation	QRKESLDSISHSSGS HCHHHHHHCCCCCCC	31.14	28857561
224	Phosphorylation	KESLDSISHSSGSED HHHHHHCCCCCCCCC	22.67	28348404
226	Phosphorylation	SLDSISHSSGSEDIE HHHHCCCCCCCCCEE	29.60	23312004
227	Phosphorylation	LDSISHSSGSEDIEV HHHCCCCCCCCCEEE	40.70	23312004
229	Phosphorylation	SISHSSGSEDIEVHH HCCCCCCCCCEEECC	33.76	23312004
237	Phosphorylation	EDIEVHHSGHKIRRN CCEEECCCCCCCCCC	27.93	23312004
253	Phosphorylation	KLTASSLSLDTASSS CEEEEEEECCCCCCC	26.58	-
278	Phosphorylation	CLLQENGSKSPDHCE EEEECCCCCCCCCCC	41.35	-
280	Phosphorylation	LQENGSKSPDHCEEP EECCCCCCCCCCCCC	36.94	25849741
289	Phosphorylation	DHCEEPMSCDSDLGT CCCCCCCCCCCCCCC	26.03	27732954
292	Phosphorylation	EEPMSCDSDLGTANA CCCCCCCCCCCCCCC	38.78	27732954
305	Phosphorylation	NAEDSDRSLQEVLLE CCCCCHHHHHHHHHH	39.12	30266825
320	O-linked_Glycosylation	FSKAQVNSVPTNGLS HHHHHHCCCCCCCCC	30.53	29351928
323	O-linked_Glycosylation	AQVNSVPTNGLSQET HHHCCCCCCCCCCCC	39.50	29351928
382	Phosphorylation	EPRPQAPSPLDLQQP CCCCCCCCCCCCCCC	40.90	25850435
431	Phosphorylation	AGLKLVVSSPTSPKN CCEEEEEECCCCCCC	24.11	30266825
432	Phosphorylation	GLKLVVSSPTSPKNK CEEEEEECCCCCCCC	22.16	30266825
434	Phosphorylation	KLVVSSPTSPKNKSW EEEEECCCCCCCCCC	60.66	30266825
435	Phosphorylation	LVVSSPTSPKNKSWI EEEECCCCCCCCCCC	36.94	30266825
440	Phosphorylation	PTSPKNKSWISEDDF CCCCCCCCCCCCCCC	38.75	22199227
443	Phosphorylation	PKNKSWISEDDFYRP CCCCCCCCCCCCCCC	28.81	28348404
468	Methylation	DHPIASYRGTPGSRP CCCCHHCCCCCCCCC	39.76	115487875
470	Phosphorylation	PIASYRGTPGSRPGL CCHHCCCCCCCCCCH	18.58	28555341
474	Methylation	YRGTPGSRPGLHRHF CCCCCCCCCCHHHCC	34.91	115487879
479	Methylation	GSRPGLHRHFSQEPR CCCCCHHHCCCCCCC	37.27	115487887
482	Phosphorylation	PGLHRHFSQEPRKNC CCHHHCCCCCCCCCC	27.75	23401153
490	Phosphorylation	QEPRKNCSLGALDQA CCCCCCCCCCCCCHH	38.95	21712546
501	Phosphorylation	LDQACVPSPGRRQAQ CCHHCCCCCCCCCHH	21.42	25159151
512	Phosphorylation	RQAQAAPSQGHKSFR CCHHCCCCCCCCCEE	44.03	24719451
516	Ubiquitination	AAPSQGHKSFRVVHR CCCCCCCCCEEEEHH	59.10	-
517	Phosphorylation	APSQGHKSFRVVHRR CCCCCCCCEEEEHHH	16.77	-
529	Phosphorylation	HRRQMGLSNPFRGLM HHHHCCCCCCCHHHH	36.41	24719451
584	Phosphorylation	CSLLRCESVGPAHSD CEEEEEEEECCCCCC	35.68	-
602	Ubiquitination	ELVFSGKKLALRASS EEEECCCEEEEECCC	42.38	-
609	Phosphorylation	KLALRASSQDEAEDW EEEEECCCHHHHHHH	40.44	30624053
720	Ubiquitination	FRIRNNEKMLSDSHG HCCCCCHHHCCCCCC	47.46	21906983
723	Phosphorylation	RNNEKMLSDSHGVET CCCHHHCCCCCCCCC	33.30	-
779	Phosphorylation	VRKVLASYLETAEEA HHHHHHHHHHHHHHH	11.71	-
788	Phosphorylation	ETAEEAVTLGGSLDE HHHHHHHHHCCCCCH	27.08	-
879	Ubiquitination	IHNWDLTKRPICRQA EECCCCCCCHHHHHH	64.57	-
926	Ubiquitination	GRRREQLKLLGDYLG CHHHHHHHHHHHHHH	41.16	-
941	Ubiquitination	LCRSGALKELSKRLN HHHHHHHHHHHHHHC	56.95	29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PKHM1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PKHM1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PKHM1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GA45G_HUMAN	GADD45G	physical	15383276

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 611497: Osteopetrosis, autosomal recessive 6 (OPTB6)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.