PIWL2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PIWL2_HUMAN
• UniProt AC: Q8TC59
• Protein Name: Piwi-like protein 2
• Gene Name: PIWIL2
• Organism: Homo sapiens (Human).
• Sequence Length: 973
• Subcellular Localization: Cytoplasm . Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
• Protein Description: Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (By similarity). During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4 (By similarity). PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded (By similarity). Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense (By similarity). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (By similarity). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. [PubMed: 16377660]
• Protein Sequence: MDPFRPSFRGQSPIHPSQCQAVRMPGCWPQASKPLDPALGRGAPAGRGHVFGKPEEPSTQRGPAQRESVGLVSMFRGLGIETVSKTPLKREMLPSGRGILGRGLSANLVRKDREELSPTFWDPKVLAAGDSKMAETSVGWSRTLGRGSSDASLLPLGRAAGGISREVDKPPCTFSTPSRGPPQLSSPPALPQSPLHSPDRPLVLTVEHKEKELIVKQGSKGTPQSLGLNLVKIQCHNEAVYQYHVTFSPNVECKSMRFGMLKDHQAVTGNVTAFDGSILYLPVKLQQVLELKSQRKTDSAEISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDCVLDVMHAIYQQNKEHFQDECTKLLVGNIVITRYNNRTYRIDDVDWNKTPKDSFTMSDGKEITFLEYYSKNYGITVKEEDQPLLIHRPSERQDNHGMLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLAQQINLSPKQHHSALECLLQRIAKNEAATNELMRWGLRLQKDVHKIEGRVLPMERINLKNTSFITSQELNWVKEVTRDPSILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRMSPPAWVELKDDRIETYVRTIQSTLGAEGKIQMVVCIIMGPRDDLYGAIKKLCCVQSPVPSQVVNVRTIGQPTRLRSVAQKILLQINCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKFYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFENYQPKMVVFVVQKKISTNLYLAAPQNFVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYVCVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHHEPAIQLCENLFFL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
32	Phosphorylation	PGCWPQASKPLDPAL CCCCCCCCCCCCHHC	29.56	24719451
47	Symmetric dimethylarginine	GRGAPAGRGHVFGKP CCCCCCCCCCCCCCC	33.86	-
47	Methylation	GRGAPAGRGHVFGKP CCCCCCCCCCCCCCC	33.86	-
76	Symmetric dimethylarginine	VGLVSMFRGLGIETV HHHHHHHCCCCCEEE	30.68	-
76	Methylation	VGLVSMFRGLGIETV HHHHHHHCCCCCEEE	30.68	19719617
97	Symmetric dimethylarginine	REMLPSGRGILGRGL HHHCCCCCCCCCCCC	33.06	-
97	Methylation	REMLPSGRGILGRGL HHHCCCCCCCCCCCC	33.06	-
102	Symmetric dimethylarginine	SGRGILGRGLSANLV CCCCCCCCCCHHHHC	39.16	-
102	Methylation	SGRGILGRGLSANLV CCCCCCCCCCHHHHC	39.16	-
105	Phosphorylation	GILGRGLSANLVRKD CCCCCCCHHHHCCCC	20.24	24732914
146	Methylation	GWSRTLGRGSSDASL CCCCCCCCCCCCHHH	44.95	30760677
148	Phosphorylation	SRTLGRGSSDASLLP CCCCCCCCCCHHHHH	24.55	22468782
152	Phosphorylation	GRGSSDASLLPLGRA CCCCCCHHHHHHHHH	35.11	22468782
158	Methylation	ASLLPLGRAAGGISR HHHHHHHHHCCCCCC	29.01	30760683
164	Phosphorylation	GRAAGGISREVDKPP HHHCCCCCCCCCCCC	26.18	22468782
165	Methylation	RAAGGISREVDKPPC HHCCCCCCCCCCCCC	46.14	-
165	Symmetric dimethylarginine	RAAGGISREVDKPPC HHCCCCCCCCCCCCC	46.14	-
222	Phosphorylation	VKQGSKGTPQSLGLN EECCCCCCCCCCCCE	23.14	-
293	Phosphorylation	QQVLELKSQRKTDSA HHHHHHHHCCCCCCC	48.09	29052541
297	Phosphorylation	ELKSQRKTDSAEISI HHHHCCCCCCCEEEE	37.06	29052541
299	Phosphorylation	KSQRKTDSAEISIKI HHCCCCCCCEEEEEE	32.79	29052541
303	Phosphorylation	KTDSAEISIKIQMTK CCCCCEEEEEEEHHH	14.88	-
309	Phosphorylation	ISIKIQMTKILEPCS EEEEEEHHHHCCCCC	9.88	-
316	Phosphorylation	TKILEPCSDLCIPFY HHHCCCCCHHHHHHH	44.31	-
422	Phosphorylation	GNIVITRYNNRTYRI CCEEEEEECCCEEEE	14.24	28509920
426	Phosphorylation	ITRYNNRTYRIDDVD EEEECCCEEEECCCC	21.19	28509920
460	Phosphorylation	LEYYSKNYGITVKEE EEEHHHHCCCEECCC	17.40	27135362
463	Phosphorylation	YSKNYGITVKEEDQP HHHHCCCEECCCCCC	22.59	27135362
551	Symmetric dimethylarginine	AATNELMRWGLRLQK HHHHHHHHHHHHHHH	36.61	-
551	Methylation	AATNELMRWGLRLQK HHHHHHHHHHHHHHH	36.61	-
637	Phosphorylation	GPIGMRMSPPAWVEL CCCCCCCCCCCEEEE	19.84	-
651	Phosphorylation	LKDDRIETYVRTIQS ECCCHHHHHHHHHHH	25.58	22461510
652	Phosphorylation	KDDRIETYVRTIQST CCCHHHHHHHHHHHH	3.83	22461510
772	Phosphorylation	NLTLTKWYSRVVFQM EEEEHHHHHHEEECC	6.53	-
931	Phosphorylation	TFKLCHMYWNWPGTI HHHHHCHHCCCCCCE	3.35	30576142

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PIWL2_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PIWL2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PIWL2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HS90A_HUMAN	HSP90AA1	physical	22848678

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.