PEDF_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PEDF_HUMAN
• UniProt AC: P36955
• Protein Name: Pigment epithelium-derived factor
• Gene Name: SERPINF1
• Organism: Homo sapiens (Human).
• Sequence Length: 418
• Subcellular Localization: Secreted . Melanosome . Enriched in stage I melanosomes.
• Protein Description: Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity..
• Protein Sequence: MQALVLLLCIGALLGHSSCQNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIHGTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVLTGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVTKFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLTGSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKLSYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGAGTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
20	Pyrrolidone_carboxylic_acid	LLGHSSCQNPASPPE HHCCCCCCCCCCCCC	61.97	-
20	Pyrrolidone_carboxylic_acid	LLGHSSCQNPASPPE HHCCCCCCCCCCCCC	61.97	12737624
20	Pyrrolidone_carboxylic_acid	LLGHSSCQNPASPPE HHCCCCCCCCCCCCC	61.97	12737624
24	Phosphorylation	SSCQNPASPPEEGSP CCCCCCCCCCCCCCC	42.09	26657352
24	O-linked_Glycosylation	SSCQNPASPPEEGSP CCCCCCCCCCCCCCC	42.09	OGP
30	O-linked_Glycosylation	ASPPEEGSPDPDSTG CCCCCCCCCCCCCCC	29.43	OGP
30	Phosphorylation	ASPPEEGSPDPDSTG CCCCCCCCCCCCCCC	29.43	26657352
35	Phosphorylation	EGSPDPDSTGALVEE CCCCCCCCCCCCCCC	33.84	26657352
36	O-linked_Glycosylation	GSPDPDSTGALVEEE CCCCCCCCCCCCCCC	33.58	OGP
36	Phosphorylation	GSPDPDSTGALVEEE CCCCCCCCCCCCCCC	33.58	26657352
114	Phosphorylation	ALYYDLISSPDIHGT HHHHHHHCCCCCCCC	43.02	22817900
152	Phosphorylation	EKKLRIKSSFVAPLE EEEHHCCEEEEECCH	27.00	-
153	Phosphorylation	KKLRIKSSFVAPLEK EEHHCCEEEEECCHH	20.81	-
227	Phosphorylation	KFDSRKTSLEDFYLD CCCCCCCCHHHHCCC	32.56	21082442
283	Phosphorylation	FFLPLKVTQNLTLIE EEEEEEECCCEEHHH	15.34	-
285	N-linked_Glycosylation	LPLKVTQNLTLIEES EEEEECCCEEHHHHH	26.15	11562499
285	N-linked_Glycosylation	LPLKVTQNLTLIEES EEEEECCCEEHHHHH	26.15	11562499
287	Phosphorylation	LKVTQNLTLIEESLT EEECCCEEHHHHHHH	32.99	-
294	Phosphorylation	TLIEESLTSEFIHDI EHHHHHHHHHHHHHH	35.17	-
295	Phosphorylation	LIEESLTSEFIHDID HHHHHHHHHHHHHHH	35.48	-
313	Phosphorylation	KTVQAVLTVPKLKLS CHHHHHEECCCCCCE	28.32	24719451
316	Methylation	QAVLTVPKLKLSYEG HHHEECCCCCCEECC	54.65	-
372	O-linked_Glycosylation	NEDGAGTTPSPGLQP CCCCCCCCCCCCCCC	22.35	OGP
374	O-linked_Glycosylation	DGAGTTPSPGLQPAH CCCCCCCCCCCCCCE	29.52	OGP

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
24	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
24	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
24	S	Phosphorylation	Kinase	CK2	-	Uniprot
114	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
114	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
114	S	Phosphorylation	Kinase	CK2	-	Uniprot
227	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
227	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
227	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PEDF_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PEDF_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PLPL2_HUMAN	PNPLA2	physical	18191271
EPMIP_HUMAN	EPM2AIP1	physical	25416956
LMBL2_HUMAN	L3MBTL2	physical	28514442
KLHL8_HUMAN	KLHL8	physical	28514442
TBA4A_HUMAN	TUBA4A	physical	28514442
GRP78_HUMAN	HSPA5	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 613982: Osteogenesis imperfecta 6 (OI6)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

N-linked Glycosylation

"Crystal structure of human PEDF, a potent anti-angiogenic and neuritegrowth-promoting factor.";
Simonovic M., Gettins P.G.W., Volz K.;
Proc. Natl. Acad. Sci. U.S.A. 98:11131-11135(2001).
Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 21-418, AND GLYCOSYLATION ATASN-285.
PubMed: 11562499

"Glycoproteomics analysis of human liver tissue by combination ofmultiple enzyme digestion and hydrazide chemistry.";
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
J. Proteome Res. 8:651-661(2009).
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-285, AND MASSSPECTROMETRY.
PubMed: 19159218

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction,hydrazide chemistry, and mass spectrometry.";
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E.,Moore R.J., Smith R.D.;
J. Proteome Res. 4:2070-2080(2005).
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-285, AND MASSSPECTROMETRY.
PubMed: 16335952

Phosphorylation

"Extracellular phosphorylation converts pigment epithelium-derivedfactor from a neurotrophic to an antiangiogenic factor.";
Maik-Rachline G., Shaltiel S., Seger R.;
Blood 105:670-678(2005).
Cited for: PHOSPHORYLATION AT SER-24; SER-114 AND SER-227.
PubMed: 15374885