dbPTM

NRIF1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NRIF1_MOUSE
UniProt AC	Q923B3
Protein Name	Neurotrophin receptor-interacting factor 1
Gene Name	Nrif1
Organism	Mus musculus (Mouse).
Sequence Length	828
Subcellular Localization	Cytoplasm. Nucleus. Translocates into the nucleus following binding to TRAF6 and subsequent ubiquitination at Lys-15.
Protein Description	Transcription regulator involved in NGFR/p75(NTR)-mediated apoptosis. Essential component of the NGFR/p75(NTR) apoptotic pathway: upon ligand-binding and subsequent cleavage of NGFR/p75(NTR), binds to the intracellular domain (ICD) cleavage product of NGFR/p75(NTR), translocates to the nucleus and induces apoptosis, possibly by regulating expression of key regulators of apoptosis. Induces NGFR/p75(NTR)-mediated apoptosis in retina and sympathetic neurons. May also regulate expression of neuronal cholesterol biosynthesis genes. Probably acts as a transcription repressor: specifically binds to the 3'-end of zinc-finger coding genes and recruiting chromatin-modifying proteins such as SETDB1 and TRIM28/KAP1, leading to transcription repression..
Protein Sequence	MASTLPTTWPHESVKFEDVSLTFTEEEWAQLDFQQKCLYREIMMENYSNMISVEHHFSKPNVISQLEKAEDCWPMQREIPQDTLPECSWPSPDPGMNSFPSKSPLMKIEVVEVLTLNKDVAGPRNALIQSLYPEDLNPGNLKPAQQPSKRLTDTEASRQKFRHFQYEESAGPQKAMSQLRKLCHQWLQPNTRSKKQILELLVLEQFLNALPEKFRVWVESQHPEDCKAVVALLENMTSVSKDDASLACSSEATDQLKEKRKGVATLPVTFAAEVPAEEPVTFQDVAVDFNEEEWRLLGPTQKTEYHDVMLETLGNLVSVGWEPTLGNRELTPDSPIPVVKPIHDPNTNDLSRNGTQSTVFESILEDGVKEMHSIESNQVGNLQEKGHPQKKFSESSKSQDQTSRHKSQGSLNEVLPRKYVKVKQKGTGKRKGRTNTISMTRGLRIRKQQKDSVEWQGRSGSTPVTHGSSIKKQQQGSEQGKPGTSRDPITLTVPAKVYQKATGSEESIFMDSSDAMVPDVPPKIHQKGPEWHKVGESNNSMLQGSSVQNHQMESGAGRASDNSLLTHALPVKSHQKGYKEGNVQGNRNSWKHIKPHQKGSKGERVEELSTSEKHVPYVKNHLKTSERGKDREINASIKCDPYIKTYYRGSDVGRLRRANNCRKAFSLHAQQISFIKIHKGSQVCRCSECGKLFRNARYFSVHKKIHTGERPYMCMACGKAFVQSSSLTQHLRIHSGERPFECSECGRTFNDRSAISQHLRTHTGAKPYHCERCGKAFRQSSHLTRHERTHTGERPYVCIKCGKAFTQSSHLIGHQKTHGIKFKKQPKL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
15	Ubiquitination	TWPHESVKFEDVSLT CCCCCCCEEEEEEEE	52.92	16630834
148	Phosphorylation	LKPAQQPSKRLTDTE CCCCCCCCCCCCCCH	26.52	22871156
154	Phosphorylation	PSKRLTDTEASRQKF CCCCCCCCHHHHHHH	28.37	23737553
157	Phosphorylation	RLTDTEASRQKFRHF CCCCCHHHHHHHHHH	29.07	23737553
334	Phosphorylation	NRELTPDSPIPVVKP CCCCCCCCCCCCCCC	26.40	-
407	Phosphorylation	DQTSRHKSQGSLNEV CHHHHHHHHCCHHHH	33.38	25367039
410	Phosphorylation	SRHKSQGSLNEVLPR HHHHHHCCHHHHCCH	22.00	25367039
434	O-linked_Glycosylation	TGKRKGRTNTISMTR CCCCCCCCCEEEECC	45.44	30059200
566	Phosphorylation	ASDNSLLTHALPVKS CCCCCCCCCCCCCCC	15.47	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of NRIF1_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
15	K	ubiquitylation		16252010
Ubiquitinated by TRAF6 at Lys-15 through 'Lys-63'-linked polyubiquitination.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NRIF1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
TRAF6_MOUSE	Traf6	physical	16252010
SQSTM_MOUSE	Sqstm1	physical	16252010

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NRIF1_MOUSE

Related Literatures of Post-Translational Modification

Ubiquitylation
Reference	PubMed
"Ligand-dependent cleavage of the P75 neurotrophin receptor isnecessary for NRIF nuclear translocation and apoptosis in sympatheticneurons."; Kenchappa R.S., Zampieri N., Chao M.V., Barker P.A., Teng H.K.,Hempstead B.L., Carter B.D.; Neuron 50:219-232(2006). Cited for: FUNCTION, UBIQUITINATION AT LYS-15, SUBCELLULAR LOCATION, ANDINTERACTION WITH NGFR.	16630834 [Abstract]
"TRAF6-mediated ubiquitination regulates nuclear translocation ofNRIF, the p75 receptor interactor."; Geetha T., Kenchappa R.S., Wooten M.W., Carter B.D.; EMBO J. 24:3859-3868(2005). Cited for: UBIQUITINATION AT LYS-15, SUBCELLULAR LOCATION, INTERACTION WITHSQSTM1 AND TRAF6, AND MUTAGENESIS OF LYS-15.	16252010 [Abstract]

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