NR1H4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NR1H4_MOUSE
• UniProt AC: Q60641
• Protein Name: Bile acid receptor
• Gene Name: Nr1h4
• Organism: Mus musculus (Mouse).
• Sequence Length: 488
• Subcellular Localization: Nucleus .
• Protein Description: Ligand-activated transcription factor. Receptor for bile acids (BAs) such as chenodeoxycholic acid (CDCA), lithocholic acid, deoxycholic acid (DCA) and allocholic acid (ACA). Plays a essential role in BA homeostasis through the regulation of genes involved in BA synthesis, conjugation and enterohepatic circulation. Also regulates lipid and glucose homeostasis and is involved in innate immune response. [PubMed: 11030617]
• Protein Sequence: MVMQFQGLENPIQISLHHSHRLSGFVPEGMSVKPAKGMLTEHAAGPLGQNLDLESYSPYNNVPFPQVQPQISSSSYYSNLGFYPQQPEDWYSPGIYELRRMPAETGYQGETEVSEMPVTKKPRMAAASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECMYTGLLTEIQCKSKRLRKNVKQHADQTANEDDSEGRDLRQVTSTTKFCREKTELTADQQTLLDYIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATSHVQILVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPAGHADLLEERIRKSGISDEYITPMFSFYKSVGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKMYQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
23	Phosphorylation	LHHSHRLSGFVPEGM EECCCCCCCCCCCCC	30.09	29233185
146	Phosphorylation	VVCGDRASGYHYNAL EEECCCCCCCCCEEE	40.55	-
165	Phosphorylation	CKGFFRRSITKNAVY CCHHHHCCCCCCCEE	30.28	-
168	Acetylation	FFRRSITKNAVYKCK HHHCCCCCCCEEECC	41.04	-
221	Methylation	TEIQCKSKRLRKNVK HHHHHCHHHHHHHHH	40.73	-
228	Acetylation	KRLRKNVKQHADQTA HHHHHHHHHHHHHHC	45.84	-
356	Ubiquitination	AEIFNKKLPAGHADL HHHHHCCCCCCCHHH	3.62	-
370	Ubiquitination	LLEERIRKSGISDEY HHHHHHHHCCCCCCH	51.14	22790023
458	Phosphorylation	GRLTELRTFNHHHAE HHHHHHHCCCHHHHH	41.22	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
146	S	Phosphorylation	Kinase	PRKCA	P20444	Uniprot
165	S	Phosphorylation	Kinase	PRKCA	P20444	Uniprot
458	T	Phosphorylation	Kinase	PRKCZ	Q02956	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
228	K	Acetylation	Lys-228 as is the major acetylation site for EP300; the dynamicly regulated acetylation inhibits heterodimerization with RXRA and transactivation activity.	18842595

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NR1H4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SMCA4_HUMAN	SMARCA4	physical	19805516
SIR1_MOUSE	Sirt1	physical	19883617
EP300_MOUSE	Ep300	physical	19883617

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.