NPC1_HUMAN - dbPTM
NPC1_HUMAN - PTM Information in dbPTM
Basic Information of Protein
UniProt ID NPC1_HUMAN
UniProt AC O15118
Protein Name NPC intracellular cholesterol transporter 1 {ECO:0000312|HGNC:HGNC:7897}
Gene Name NPC1 {ECO:0000312|HGNC:HGNC:7897}
Organism Homo sapiens (Human).
Sequence Length 1278
Subcellular Localization Late endosome membrane
Multi-pass membrane protein . Lysosome membrane
Multi-pass membrane protein .
Protein Description Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.; (Microbial infection) Acts as an endosomal entry receptor for ebolavirus..
Protein Sequence MTARGLALGLLLLLLCPAQVFSQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPLQFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLDAMYVIMWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGEASCCDPVSAAFEGCLRRLFTRWGSFCVRNPGCVIFFSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGLPLTLIVIEVIPFLVLAVGVDNIFILVQAYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTSVQASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYFDWVKPQSSCCRVDNITDQFCNASVVDPACVRCRPLTPEGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTVLQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCATEERYKGTERERLLNF
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure		 ASA (%) Reference Orthologous
Protein Cluster
49UbiquitinationCEYSGPPKPLPKDGY
CCCCCCCCCCCCCCH		63.4229967540
70N-linked_GlycosylationCPGFFFGNVSLCCDV
CCCCCCCCEEEECCH		18.3010821832
84UbiquitinationVRQLQTLKDNLQLPL
HHHHHHHHHHCHHCH		48.2121906983
95PhosphorylationQLPLQFLSRCPSCFY
HHCHHHHHCCCHHHH		32.8821712546
122N-linked_GlycosylationPRQSQFLNVTATEDY
CCHHHCCCEEECCCC		30.3110821832
135N-linked_GlycosylationDYVDPVTNQTKTNVK
CCCCCCCCCCCCCHH		48.0027238017
142UbiquitinationNQTKTNVKELQYYVG
CCCCCCHHHHHHHHH		55.57-
158N-linked_GlycosylationSFANAMYNACRDVEA
HHHHHHHHHHHCCCC		21.3419563754
171UbiquitinationEAPSSNDKALGLLCG
CCCCCCCCCHHHHHC		50.1629967540
185N-linked_GlycosylationGKDADACNATNWIEY
CCCHHHCCCCHHHHH		52.0710821832
222N-linked_GlycosylationHGMEPMNNATKGCDE
CCCCCCCCCCCCCCC		42.6119563754
254UbiquitinationCGPKPQPPPPPAPWT
ECCCCCCCCCCCCCC		49.1022817900
297PhosphorylationVWCYRKRYFVSEYTP
HHHHHHHHEEEECCC		16.0329888752
300PhosphorylationYRKRYFVSEYTPIDS
HHHHHEEEECCCCCC		18.2629888752
302PhosphorylationKRYFVSEYTPIDSNI
HHHEEEECCCCCCCE		15.4329888752
303PhosphorylationRYFVSEYTPIDSNIA
HHEEEECCCCCCCEE		15.0929888752
307PhosphorylationSEYTPIDSNIAFSVN
EECCCCCCCEEEEEE		30.7529888752
312PhosphorylationIDSNIAFSVNASDKG
CCCCEEEEEECCCCC		12.8729888752
316PhosphorylationIAFSVNASDKGEASC
EEEEEECCCCCCCCC		34.3529888752
382PhosphorylationTNPVDLWSAPSSQAR
CCCCCCCCCCCHHHH		37.2825599653
385PhosphorylationVDLWSAPSSQARLEK
CCCCCCCCHHHHHHH		34.50-
386PhosphorylationDLWSAPSSQARLEKE
CCCCCCCHHHHHHHH		27.14-
392UbiquitinationSSQARLEKEYFDQHF
CHHHHHHHHHHHHHC		63.4121906983
394PhosphorylationQARLEKEYFDQHFGP
HHHHHHHHHHHHCCC		24.43-
409UbiquitinationFFRTEQLIIRAPLTD
CCCCCEEEEECCCCC		1.6822817900
447UbiquitinationLHQVLDLQIAIENIT
HHHHHHHHHHHEEEC		23.8021963094
452N-linked_GlycosylationDLQIAIENITASYDN
HHHHHHEEECEECCC		30.6928784760
459N-linked_GlycosylationNITASYDNETVTLQD
EECEECCCCCEEEEE		38.3128784760
478N-linked_GlycosylationPLSPYNTNCTILSVL
CCCCCCCCCCHHHHH		19.8028784760
504UbiquitinationHKKGDDFFVYADYHT
CCCCCCEEEEEECCC		5.44-
524N-linked_GlycosylationVRAPASLNDTSLLHD
EECCCCCCCCCCCCC		48.2826846330
557N-linked_GlycosylationYDDQNYNNATALVIT
CCCCCCCCCEEEEEE		28.4528784760
564PhosphorylationNATALVITFPVNNYY
CCEEEEEEEECCCCC		17.8828787133
570PhosphorylationITFPVNNYYNDTEKL
EEEECCCCCCCHHHH		9.8428787133
571PhosphorylationTFPVNNYYNDTEKLQ
EEECCCCCCCHHHHH		14.5328787133
572N-linked_GlycosylationFPVNNYYNDTEKLQR
EECCCCCCCHHHHHH		39.0926846330
574PhosphorylationVNNYYNDTEKLQRAQ
CCCCCCCHHHHHHHH		31.2328787133
585UbiquitinationQRAQAWEKEFINFVK
HHHHHHHHHHHHHHH		47.4221906983
598N-linked_GlycosylationVKNYKNPNLTISFTA
HHHCCCCCEEEEEEE		60.2428784760
602UbiquitinationKNPNLTISFTAERSI
CCCCEEEEEEECCCH		16.0021963094
684UbiquitinationYIGLPLTLIVIEVIP
HCCCCHHHHHHHHHH		3.7622817900
692UbiquitinationIVIEVIPFLVLAVGV
HHHHHHHHHHHHHCC		5.18-
799S-palmitoylationKNRLDIFCCVRGAED
HCCCEEEEEEECCCC		1.8329575903
800S-palmitoylationNRLDIFCCVRGAEDG
CCCEEEEEEECCCCC		1.3029575903
822UbiquitinationSCLFRFFKNSYSPLL
HHHHHHHHCCCCCHH		42.1221906983
826PhosphorylationRFFKNSYSPLLLKDW
HHHHCCCCCHHHHHC		14.64-
839UbiquitinationDWMRPIVIAIFVGVL
HCHHHHHHHHHHHHH		2.2322817900
862UbiquitinationKVDIGLDQSLSMPDD
CCCCCCCCCCCCCCC		51.8421890473
903AcetylationGHDYTSSKGQNMVCG
CCCCCCCCCCCEECC		65.3730590875
916N-linked_GlycosylationCGGMGCNNDSLVQQI
CCCCCCCCHHHHHHH		44.3028784760
931N-linked_GlycosylationFNAAQLDNYTRIGFA
HHHHHCCCCCEEECC		48.9828784760
961N-linked_GlycosylationSSCCRVDNITDQFCN
CCCCCCCCCCHHCCC		35.8828784760
963PhosphorylationCCRVDNITDQFCNAS
CCCCCCCCHHCCCCC		29.9730576142
968N-linked_GlycosylationNITDQFCNASVVDPA
CCCHHCCCCCCCCHH		36.2228784760
987UbiquitinationRPLTPEGKQRPQGGD
EECCCCCCCCCCCCC		41.2829967540
1010UbiquitinationLSDNPNPKCGKGGHA
HCCCCCCCCCCCHHH		63.36-
1042UbiquitinationATYFMTYHTVLQTSA
CEEEECHHHHHHCCH		10.5927667366
1064N-linked_GlycosylationKARLIASNVTETMGI
HHHHHHCCCCCCCCC		35.3928784760
1072N-linked_GlycosylationVTETMGINGSAYRVF
CCCCCCCCCCCEECC		32.41UniProtKB CARBOHYD
1180UbiquitinationRAFTVSMKGSRVERA
HCEEEEECCCHHHHH		46.3532142685
1182PhosphorylationFTVSMKGSRVERAEE
EEEEECCCHHHHHHH		27.8628985074
1196PhosphorylationEALAHMGSSVFSGIT
HHHHHCCCCCCCCCE		18.7628985074
1197PhosphorylationALAHMGSSVFSGITL
HHHHCCCCCCCCCEE		22.8128985074
1197UbiquitinationALAHMGSSVFSGITL
HHHHCCCCCCCCCEE		22.8127667366
1259UbiquitinationGPSVNKAKSCATEER
CCCCCCHHCCCCCHH		47.9133845483
1270PhosphorylationTEERYKGTERERLLN
CCHHHCCCHHHHHHC		27.9320068231
Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources

Oops, there are no upstream regulatory protein records of NPC1_HUMAN !!
Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of NPC1_HUMAN !!
Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10) 		Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of NPC1_HUMAN !!
Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
RT28_HUMANMRPS28physical
22939629
TMOD3_HUMANTMOD3physical
22939629
Drug and Disease Associations
Kegg Disease
H00136 Niemann-Pick type C disease (NPC), including: Niemann-Pick disease, type C1; Niemann-Pick disease, t
OMIM Disease
257220Niemann-Pick disease C1 (NPC1)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of NPC1_HUMAN

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Related Literatures of Post-Translational Modification
N-linked Glycosylation
ReferencePubMed
"Glycoproteomics analysis of human liver tissue by combination ofmultiple enzyme digestion and hydrazide chemistry.";
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
J. Proteome Res. 8:651-661(2009).
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-135 AND ASN-524, AND MASSSPECTROMETRY.

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