NHX1_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NHX1_ARATH
• UniProt AC: Q68KI4
• Protein Name: Sodium/hydrogen exchanger 1
• Gene Name: NHX1
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 538
• Subcellular Localization: Vacuole membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Tonoplast. Possibly also present in endoplasmic reticulum (ER) and Golgi apparatu
• Protein Description: Acts in low affinity electroneutral exchange of protons for cations such as Na(+) or K(+) across membranes. Can also exchange Li(+) and Cs(+) with a lower affinity. Involved in vacuolar ion compartmentalization necessary for cell volume regulation and cytoplasmic Na(+) detoxification. Required during leaves expansion, probably to stimulate epidermal cell expansion. Confers competence to grow in high salinity conditions..
• Protein Sequence: MLDSLVSKLPSLSTSDHASVVALNLFVALLCACIVLGHLLEENRWMNESITALLIGLGTGVTILLISKGKSSHLLVFSEDLFFIYLLPPIIFNAGFQVKKKQFFRNFVTIMLFGAVGTIISCTIISLGVTQFFKKLDIGTFDLGDYLAIGAIFAATDSVCTLQVLNQDETPLLYSLVFGEGVVNDATSVVVFNAIQSFDLTHLNHEAAFHLLGNFLYLFLLSTLLGAATGLISAYVIKKLYFGRHSTDREVALMMLMAYLSYMLAELFDLSGILTVFFCGIVMSHYTWHNVTESSRITTKHTFATLSFLAETFIFLYVGMDALDIDKWRSVSDTPGTSIAVSSILMGLVMVGRAAFVFPLSFLSNLAKKNQSEKINFNMQVVIWWSGLMRGAVSMALAYNKFTRAGHTDVRGNAIMITSTITVCLFSTVVFGMLTKPLISYLLPHQNATTSMLSDDNTPKSIHIPLLDQDSFIEPSGNHNVPRPDSIRGFLTRPTRTVHYYWRQFDDSFMRPVFGGRGFVPFVPGSPTERNPPDLSKA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
290	N-linked_Glycosylation	MSHYTWHNVTESSRI HHHCCCCCCCCCCCC	33.69	-
370	N-linked_Glycosylation	LSNLAKKNQSEKINF HHHHHHHCHHHCCCC	50.32	-
399	Phosphorylation	AVSMALAYNKFTRAG HHHHHHHHHHHCCCC	21.53	24894044
447	N-linked_Glycosylation	SYLLPHQNATTSMLS HHHCCCCCCCCCCCC	35.90	-
461	Phosphorylation	SDDNTPKSIHIPLLD CCCCCCCEEEEEECC	22.17	27643528
471	Phosphorylation	IPLLDQDSFIEPSGN EEECCCCCCCCCCCC	23.61	27643528
476	Phosphorylation	QDSFIEPSGNHNVPR CCCCCCCCCCCCCCC	39.16	27643528
486	Phosphorylation	HNVPRPDSIRGFLTR CCCCCCCCCCEECCC	19.50	27643528
526	Phosphorylation	FVPFVPGSPTERNPP CCCCCCCCCCCCCCC	23.68	30291188
528	Phosphorylation	PFVPGSPTERNPPDL CCCCCCCCCCCCCCC	49.64	19880383
536	Phosphorylation	ERNPPDLSKA----- CCCCCCCCCC-----	33.64	19376835

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NHX1_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NHX1_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NHX1_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GONS1_ARATH	GONST1	physical	22737156
CAAT9_ARATH	CAT9	physical	22737156
ERDL4_ARATH	AT1G19450	physical	22737156
CML18_ARATH	AT3G03000	physical	16249341

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale Arabidopsis phosphoproteome profiling reveals novelchloroplast kinase substrates and phosphorylation networks.";
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,Grossmann J., Gruissem W., Baginsky S.;
Plant Physiol. 150:889-903(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-526, AND MASSSPECTROMETRY.
PubMed: 19376835