dbPTM

NFL_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFL_MOUSE
UniProt AC	P08551
Protein Name	Neurofilament light polypeptide
Gene Name	Nefl
Organism	Mus musculus (Mouse).
Sequence Length	543
Subcellular Localization
Protein Description	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber..
Protein Sequence	MSSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKEESAGEEQVAKKKD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSSFGYDPY ------CCCCCCCCC	34.08	-
2	Phosphorylation	------MSSFGYDPY ------CCCCCCCCC	34.08	27149854
3	Phosphorylation	-----MSSFGYDPYF -----CCCCCCCCCC	22.76	27149854
6	Phosphorylation	--MSSFGYDPYFSTS --CCCCCCCCCCCCC	16.25	27149854
9	Phosphorylation	SSFGYDPYFSTSYKR CCCCCCCCCCCCHHC	13.78	27149854
11	Phosphorylation	FGYDPYFSTSYKRRY CCCCCCCCCCHHCCC	15.59	27149854
12	Phosphorylation	GYDPYFSTSYKRRYV CCCCCCCCCHHCCCC	26.61	27149854
13	Phosphorylation	YDPYFSTSYKRRYVE CCCCCCCCHHCCCCC	27.71	27149854
14	Phosphorylation	DPYFSTSYKRRYVET CCCCCCCHHCCCCCC	14.49	27149854
15	Ubiquitination	PYFSTSYKRRYVETP CCCCCCHHCCCCCCC	30.11	22790023
18	Nitration	STSYKRRYVETPRVH CCCHHCCCCCCCCEE	13.48	-
21	O-linked_Glycosylation	YKRRYVETPRVHISS HHCCCCCCCCEEHHH	13.32	-
23	Asymmetric dimethylarginine	RRYVETPRVHISSVR CCCCCCCCEEHHHCC	39.81	-
23	Methylation	RRYVETPRVHISSVR CCCCCCCCEEHHHCC	39.81	24129315
27	O-linked_Glycosylation	ETPRVHISSVRSGYS CCCCEEHHHCCCCCC	14.11	-
27	Phosphorylation	ETPRVHISSVRSGYS CCCCEEHHHCCCCCC	14.11	22817900
28	Phosphorylation	TPRVHISSVRSGYST CCCEEHHHCCCCCCC	22.43	20415495
30	Methylation	RVHISSVRSGYSTAR CEEHHHCCCCCCCHH	26.72	24129315
34	Phosphorylation	SSVRSGYSTARSAYS HHCCCCCCCHHHHHH	21.08	-
38	Phosphorylation	SGYSTARSAYSSYSA CCCCCHHHHHHCCCC	29.15	21183079
42	Phosphorylation	TARSAYSSYSAPVSS CHHHHHHCCCCCCCC	15.49	-
43	Phosphorylation	ARSAYSSYSAPVSSS HHHHHHCCCCCCCCC	11.69	22817900
44	Phosphorylation	RSAYSSYSAPVSSSL HHHHHCCCCCCCCCC	28.22	24759943
48	O-linked_Glycosylation	SSYSAPVSSSLSVRR HCCCCCCCCCCEEEC	17.10	22517741
48	Phosphorylation	SSYSAPVSSSLSVRR HCCCCCCCCCCEEEC	17.10	29899451
49	Phosphorylation	SYSAPVSSSLSVRRS CCCCCCCCCCEEECC	35.22	29899451
49	O-linked_Glycosylation	SYSAPVSSSLSVRRS CCCCCCCCCCEEECC	35.22	8510535
50	O-linked_Glycosylation	YSAPVSSSLSVRRSY CCCCCCCCCEEECCC	19.70	51551
50	Phosphorylation	YSAPVSSSLSVRRSY CCCCCCCCCEEECCC	19.70	29899451
52	Phosphorylation	APVSSSLSVRRSYSS CCCCCCCEEECCCCC	18.61	-
52	O-linked_Glycosylation	APVSSSLSVRRSYSS CCCCCCCEEECCCCC	18.61	50653
56	Phosphorylation	SSLSVRRSYSSSSGS CCCEEECCCCCCCCC	20.85	25521595
57	Phosphorylation	SLSVRRSYSSSSGSL CCEEECCCCCCCCCC	15.68	19060867
58	Phosphorylation	LSVRRSYSSSSGSLM CEEECCCCCCCCCCC	25.62	22817900
59	Phosphorylation	SVRRSYSSSSGSLMP EEECCCCCCCCCCCC	21.93	29899451
60	Phosphorylation	VRRSYSSSSGSLMPS EECCCCCCCCCCCCC	32.55	23608596
61	Phosphorylation	RRSYSSSSGSLMPSL ECCCCCCCCCCCCCC	34.06	19060867
63	Phosphorylation	SYSSSSGSLMPSLEN CCCCCCCCCCCCCCC	24.36	21183079
67	Phosphorylation	SSGSLMPSLENLDLS CCCCCCCCCCCCCHH	34.06	21183079
74	Phosphorylation	SLENLDLSQVAAISN CCCCCCHHHHHHHHH	23.44	29899451
91	Ubiquitination	KSIRTQEKAQLQDLN HHHHHHHHHHHHHHH	31.45	27667366
103	Phosphorylation	DLNDRFASFIERVHE HHHHHHHHHHHHHHH	24.84	22817900
139	Nitration	PSRFRALYEQEIRDL CHHHHHHHHHHHHHH	18.00	-
157	Ubiquitination	AEDATNEKQALQGER HHHCCCHHHHHHHHH	42.83	27667366
178	Nitration	LRNLQARYEEEVLSR HHHHHHHHHHHHHCH	30.61	-
184	Phosphorylation	RYEEEVLSREDAEGR HHHHHHHCHHHHHHH	38.51	29899451
197	Ubiquitination	GRLMEARKGADEAAL HHHHHHHCCCCHHHH	66.55	27667366
211	Ubiquitination	LARAELEKRIDSLMD HHHHHHHHHHHHHHH	68.24	27667366
215	Phosphorylation	ELEKRIDSLMDEIAF HHHHHHHHHHHHHHH	24.19	29899451
266	Nitration	LKDIRAQYEKLAAKN HHHHHHHHHHHHHHH	17.66	-
268	Ubiquitination	DIRAQYEKLAAKNMQ HHHHHHHHHHHHHCC	38.19	22790023
272	Ubiquitination	QYEKLAAKNMQNAEE HHHHHHHHHCCCHHH	47.55	22790023
282	Ubiquitination	QNAEEWFKSRFTVLT CCHHHHHHHHHHHHC	41.62	22790023
289	Phosphorylation	KSRFTVLTESAAKNT HHHHHHHCHHHHCCC	24.45	20415495
291	Phosphorylation	RFTVLTESAAKNTDA HHHHHCHHHHCCCHH	28.43	22817900
294	Ubiquitination	VLTESAAKNTDAVRA HHCHHHHCCCHHHHH	61.16	22790023
303	Ubiquitination	TDAVRAAKDEVSESR CHHHHHHHHHHHHHH	54.13	27667366
307	Phosphorylation	RAAKDEVSESRRLLK HHHHHHHHHHHHHHH	28.01	22324799
309	Phosphorylation	AKDEVSESRRLLKAK HHHHHHHHHHHHHHH	18.39	23737553
316	Ubiquitination	SRRLLKAKTLEIEAC HHHHHHHHHHHHHHH	52.64	22790023
317	Phosphorylation	RRLLKAKTLEIEACR HHHHHHHHHHHHHHH	34.19	29899451
323	S-nitrosocysteine	KTLEIEACRGMNEAL HHHHHHHHHCHHHHH	2.21	-
323	S-nitrosylation	KTLEIEACRGMNEAL HHHHHHHHHCHHHHH	2.21	22178444
332	Ubiquitination	GMNEALEKQLQELED CHHHHHHHHHHHHHH	58.10	22790023
346	Phosphorylation	DKQNADISAMQDTIN HHHHCCHHHHHHHHH	20.45	25521595
369	Nitration	TKSEMARYLKEYQDL CHHHHHHHHHHHHHH	16.76	-
371	Ubiquitination	SEMARYLKEYQDLLN HHHHHHHHHHHHHHC	45.50	22790023
392	Ubiquitination	IEIAAYRKLLEGEET HHHHHHHHHHCCCCC	44.76	-
402	Phosphorylation	EGEETRLSFTSVGSI CCCCCEEEEEECCCC	24.16	29899451
411	Phosphorylation	TSVGSITSGYSQSSQ EECCCCCCCCCCCCC	33.78	29899451
414	O-linked_Glycosylation	GSITSGYSQSSQVFG CCCCCCCCCCCCHHC	27.41	22517741
423	Phosphorylation	SSQVFGRSAYSGLQS CCCHHCCHHCCCCCC	31.75	25177544
423	O-linked_Glycosylation	SSQVFGRSAYSGLQS CCCHHCCHHCCCCCC	31.75	55412861
425	Phosphorylation	QVFGRSAYSGLQSSS CHHCCHHCCCCCCHH	12.43	22817900
426	Phosphorylation	VFGRSAYSGLQSSSY HHCCHHCCCCCCHHH	32.51	29899451
430	Phosphorylation	SAYSGLQSSSYLMSA HHCCCCCCHHHHHHH	26.71	25521595
433	Phosphorylation	SGLQSSSYLMSARSF CCCCCHHHHHHHCCC	14.36	22817900
436	Phosphorylation	QSSSYLMSARSFPAY CCHHHHHHHCCCCCH	20.24	-
439	Phosphorylation	SYLMSARSFPAYYTS HHHHHHCCCCCHHCC	34.99	29899451
453	Phosphorylation	SHVQEEQTEVEETIE CCCHHHHHHHHHHHH	45.32	29899451
468	Ubiquitination	ATKAEEAKDEPPSEG HHHHHHHCCCCCCCC	66.94	27667366
473	Phosphorylation	EAKDEPPSEGEAEEE HHCCCCCCCCCHHHH	69.79	25521595
503	Phosphorylation	EEAAKDESEDTKEEE HHHHHCCCCCCHHHH	51.40	25521595
506	Phosphorylation	AKDESEDTKEEEEGG HHCCCCCCHHHHCCC	35.98	25521595
520	Phosphorylation	GEGEEEDTKESEEEE CCCCCCCCHHHHHHH	39.74	25521595
523	Phosphorylation	EEEDTKESEEEEKKE CCCCCHHHHHHHHHH	52.44	25521595
532	Phosphorylation	EEEKKEESAGEEQVA HHHHHHHHHHHHHHH	42.54	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
56	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
58	S	Phosphorylation	Kinase	CAMK2A	P11798	PSP
473	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
473	S	Phosphorylation	Kinase	PLK1	P53350	PSP
473	S	Phosphorylation	Kinase	PLK3	Q9H4B4	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	Trim2	Q9ESN6	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFL_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFL_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TRIM2_MOUSE	Trim2	physical	18687884
NDEL1_MOUSE	Ndel1	physical	15208636
NFH_MOUSE	Nefh	physical	15208636

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFL_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-532, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations."; Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.; Mol. Cell. Proteomics 5:914-922(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-473 AND SER-532, ANDMASS SPECTROMETRY.	16452087 [Abstract]
"Identification of Ser-55 as a major protein kinase A phosphorylationsite on the 70-kDa subunit of neurofilaments. Early turnover duringaxonal transport."; Sihag R.K., Nixon R.A.; J. Biol. Chem. 266:18861-18867(1991). Cited for: PROTEIN SEQUENCE OF 52-57, AND PHOSPHORYLATION AT SER-56.	1717455 [Abstract]
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-43; TYR-425 AND TYR-433,AND MASS SPECTROMETRY.	18034455 [Abstract]