dbPTM

NFH_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFH_MOUSE
UniProt AC	P19246
Protein Name	Neurofilament heavy polypeptide
Gene Name	Nefh
Organism	Mus musculus (Mouse).
Sequence Length	1090
Subcellular Localization	Cytoplasm .
Protein Description	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins..
Protein Sequence	MMSFGSADALLGAPFAPLHGGGSLHYSLSRKAGPGGTRSAAGSSSGFHSWARTSVSSVSASPSRFRGAASSTDSLDTLSNGPEGCVVAAVAARSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDEEARQREEAEAAARALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQGCGAAQAQAQAEARDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKESLERQRSELEDRHQADIASYQDAIQQLDSELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFSLTEGLPKIPSISTHIKVKSEEMIKVVEKSEKETVIVEGQTEEIRVTEGVTEEEDKEAQGQEGEEAEEGEEKEEEEGAAATSPPAEEAASPEKETKSRVKEEAKSPGEAKSPGEAKSPAEAKSPGEAKSPGEAKSPGEAKSPAEPKSPAEPKSPAEAKSPAEPKSPATVKSPGEAKSPSEAKSPAEAKSPAEAKSPAEAKSPAEAKSPAEAKSPAEAKSPATVKSPGEAKSPSEAKSPAEAKSPAEAKSPAEAKSPAEVKSPGEAKSPAEPKSPAEAKSPAEVKSPAEAKSPAEVKSPGEAKSPAAVKSPAEAKSPAAVKSPGEAKSPGEAKSPAEAKSPAEAKSPIEVKSPEKAKTPVKEGAKSPAEAKSPEKAKSPVKEDIKPPAEAKSPEKAKSPVKEGAKPPEKAKPLDVKSPEAQTPVQEEAKHPTDIRPPEQVKSPAKEKAKSPEKEEAKTSEKVAPKKEEVKSPVKEEVKAKEPPKKVEEEKTLPTPKTEAKESKKDEAPKEAPKPKVEEKKETPTEKPKDSTAEAKKEEAGEKKKAVASEEETPAKLGVKEEAKPKEKTETTKTEAEDTKAKEPSKPTETEKPKKEEMPAAPEKKDTKEEKTTESRKPEEKPKMEAKVKEDDKSLSKEPSKPKTEKAEKSSSTDQKESQPPEKTTEDKATKGEK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
39	O-linked_Glycosylation	AGPGGTRSAAGSSSG CCCCCCCCCCCCCCC	23.47	55412853
44	Phosphorylation	TRSAAGSSSGFHSWA CCCCCCCCCCCCCCC	33.30	25521595
53	O-linked_Glycosylation	GFHSWARTSVSSVSA CCCCCCCCCCCCCCC	26.23	8702840
53	Phosphorylation	GFHSWARTSVSSVSA CCCCCCCCCCCCCCC	26.23	24759943
54	O-linked_Glycosylation	FHSWARTSVSSVSAS CCCCCCCCCCCCCCC	17.91	8702840
54	Phosphorylation	FHSWARTSVSSVSAS CCCCCCCCCCCCCCC	17.91	25521595
56	O-linked_Glycosylation	SWARTSVSSVSASPS CCCCCCCCCCCCCHH	25.45	8702840
56	Phosphorylation	SWARTSVSSVSASPS CCCCCCCCCCCCCHH	25.45	29899451
57	Phosphorylation	WARTSVSSVSASPSR CCCCCCCCCCCCHHH	20.24	29899451
59	Phosphorylation	RTSVSSVSASPSRFR CCCCCCCCCCHHHCC	25.61	24925903
61	Phosphorylation	SVSSVSASPSRFRGA CCCCCCCCHHHCCCC	18.85	25521595
63	Phosphorylation	SSVSASPSRFRGAAS CCCCCCHHHCCCCCC	41.37	25521595
70	Phosphorylation	SRFRGAASSTDSLDT HHCCCCCCCCCCHHH	32.98	24925903
71	Phosphorylation	RFRGAASSTDSLDTL HCCCCCCCCCCHHHH	31.35	25521595
72	Phosphorylation	FRGAASSTDSLDTLS CCCCCCCCCCHHHHH	27.41	24925903
74	Phosphorylation	GAASSTDSLDTLSNG CCCCCCCCHHHHHCC	28.38	24925903
77	Phosphorylation	SSTDSLDTLSNGPEG CCCCCHHHHHCCCCH	37.06	24925903
79	Phosphorylation	TDSLDTLSNGPEGCV CCCHHHHHCCCCHHH	41.66	29899451
96	Ubiquitination	AVAARSEKEQLQALN HHHCCCHHHHHHHHH	52.59	22790023
122	Phosphorylation	QLEAHNRSLEGEAAA HHHHHCCCHHHHHHH	35.77	25521595
225	S-nitrosocysteine	AQALQEECGYLRRHH HHHHHHHHCHHHHHH	4.23	-
225	S-nitrosylation	AQALQEECGYLRRHH HHHHHHHHCHHHHHH	4.23	22178444
262	Ubiquitination	AEARDALKCDVTSAL HHHHHHHHCHHHHHH	30.06	22790023
263	S-nitrosocysteine	EARDALKCDVTSALR HHHHHHHCHHHHHHH	5.45	-
263	S-nitrosylation	EARDALKCDVTSALR HHHHHHHCHHHHHHH	5.45	22178444
299	Phosphorylation	RVRLDRLSEAAKVNT HHHHHHHHHHHHCCH	26.53	29899451
303	Ubiquitination	DRLSEAAKVNTDAMR HHHHHHHHCCHHHHH	42.96	22790023
319	Phosphorylation	AQEEITEYRRQLQAR HHHHHHHHHHHHHHH	11.49	29899451
345	Phosphorylation	ESLERQRSELEDRHQ HHHHHHHHHHHHHHH	37.89	29899451
358	Nitration	HQADIASYQDAIQQL HHHHHHHHHHHHHHH	11.03	-
367	Phosphorylation	DAIQQLDSELRNTKW HHHHHHHHHHHHCHH	47.31	29899451
402	Ubiquitination	IEIAAYRKLLEGEEC HHHHHHHHHHCCCCC	44.76	22790023
416	Phosphorylation	CRIGFGPSPFSLTEG CCCCCCCCCCCCCCC	38.67	24925903
419	Phosphorylation	GFGPSPFSLTEGLPK CCCCCCCCCCCCCCC	37.45	24925903
421	Phosphorylation	GPSPFSLTEGLPKIP CCCCCCCCCCCCCCC	27.15	24925903
426	Ubiquitination	SLTEGLPKIPSISTH CCCCCCCCCCCCCCE	72.75	-
429	Phosphorylation	EGLPKIPSISTHIKV CCCCCCCCCCCEEEE	32.42	22817900
431	O-linked_Glycosylation	LPKIPSISTHIKVKS CCCCCCCCCEEEECC	20.47	55412845
431	Phosphorylation	LPKIPSISTHIKVKS CCCCCCCCCEEEECC	20.47	28066266
432	Phosphorylation	PKIPSISTHIKVKSE CCCCCCCCEEEECCH	26.42	28066266
438	Phosphorylation	STHIKVKSEEMIKVV CCEEEECCHHHHHEE	41.60	25521595
443	Ubiquitination	VKSEEMIKVVEKSEK ECCHHHHHEEECCCC	38.63	22790023
469	Phosphorylation	IRVTEGVTEEEDKEA EEEECCCCHHHHHHH	48.84	25521595
499	Phosphorylation	EEEGAAATSPPAEEA HHHCCCCCCCCHHHH	36.72	25521595
500	Phosphorylation	EEGAAATSPPAEEAA HHCCCCCCCCHHHHC	24.94	25521595
508	Phosphorylation	PPAEEAASPEKETKS CCHHHHCCCHHHHHH	40.41	25521595
513	Phosphorylation	AASPEKETKSRVKEE HCCCHHHHHHHHHHH	45.44	29899451
523	Phosphorylation	RVKEEAKSPGEAKSP HHHHHHCCCCCCCCC	45.22	25521595
529	Phosphorylation	KSPGEAKSPGEAKSP CCCCCCCCCCCCCCH	45.22	25521595
535	Phosphorylation	KSPGEAKSPAEAKSP CCCCCCCCHHHCCCC	36.07	25521595
541	Phosphorylation	KSPAEAKSPGEAKSP CCHHHCCCCCCCCCC	45.22	25521595
547	Phosphorylation	KSPGEAKSPGEAKSP CCCCCCCCCCCCCCC	45.22	25521595
553	Phosphorylation	KSPGEAKSPGEAKSP CCCCCCCCCCCCCCC	45.22	25521595
558	Acetylation	AKSPGEAKSPAEPKS CCCCCCCCCCCCCCC	53.31	15618231
559	Phosphorylation	KSPGEAKSPAEPKSP CCCCCCCCCCCCCCC	36.07	25521595
565	Phosphorylation	KSPAEPKSPAEPKSP CCCCCCCCCCCCCCH	39.81	25521595
571	Phosphorylation	KSPAEPKSPAEAKSP CCCCCCCCHHHCCCC	39.81	25521595
577	Phosphorylation	KSPAEAKSPAEPKSP CCHHHCCCCCCCCCC	36.07	25521595
583	Phosphorylation	KSPAEPKSPATVKSP CCCCCCCCCCCCCCC	30.40	25521595
586	Phosphorylation	AEPKSPATVKSPGEA CCCCCCCCCCCCCCC	31.67	24925903
589	Phosphorylation	KSPATVKSPGEAKSP CCCCCCCCCCCCCCH	33.53	25521595
595	Phosphorylation	KSPGEAKSPSEAKSP CCCCCCCCHHHCCCH	40.48	25521595
597	Phosphorylation	PGEAKSPSEAKSPAE CCCCCCHHHCCCHHH	58.22	25521595
601	Phosphorylation	KSPSEAKSPAEAKSP CCHHHCCCHHHCCCH	36.07	25521595
607	Phosphorylation	KSPAEAKSPAEAKSP CCHHHCCCHHHCCCH	36.07	25521595
613	Phosphorylation	KSPAEAKSPAEAKSP CCHHHCCCHHHCCCH	36.07	25521595
619	Phosphorylation	KSPAEAKSPAEAKSP CCHHHCCCHHHCCCH	36.07	25521595
625	Phosphorylation	KSPAEAKSPAEAKSP CCHHHCCCHHHCCCH	36.07	25521595
631	Phosphorylation	KSPAEAKSPAEAKSP CCHHHCCCHHHCCCC	36.07	25521595
637	Phosphorylation	KSPAEAKSPATVKSP CCHHHCCCCCCCCCC	27.15	25521595
640	Phosphorylation	AEAKSPATVKSPGEA HHCCCCCCCCCCCCC	31.67	24925903
643	Phosphorylation	KSPATVKSPGEAKSP CCCCCCCCCCCCCCH	33.53	25521595
649	Phosphorylation	KSPGEAKSPSEAKSP CCCCCCCCHHHCCCH	40.48	25521595
651	Phosphorylation	PGEAKSPSEAKSPAE CCCCCCHHHCCCHHH	58.22	25521595
655	Phosphorylation	KSPSEAKSPAEAKSP CCHHHCCCHHHCCCH	36.07	25521595
661	Phosphorylation	KSPAEAKSPAEAKSP CCHHHCCCHHHCCCH	36.07	25521595
667	Phosphorylation	KSPAEAKSPAEAKSP CCHHHCCCHHHCCCC	36.07	25521595
673	Phosphorylation	KSPAEAKSPAEVKSP CCHHHCCCCCCCCCC	36.07	25521595
679	Phosphorylation	KSPAEVKSPGEAKSP CCCCCCCCCCCCCCC	43.23	25521595
684	Acetylation	VKSPGEAKSPAEPKS CCCCCCCCCCCCCCC	53.31	15618241
685	Phosphorylation	KSPGEAKSPAEPKSP CCCCCCCCCCCCCCH	36.07	25521595
691	Phosphorylation	KSPAEPKSPAEAKSP CCCCCCCCHHHCCCH	39.81	25521595
697	Phosphorylation	KSPAEAKSPAEVKSP CCHHHCCCHHHCCCH	36.07	25521595
703	Phosphorylation	KSPAEVKSPAEAKSP CCHHHCCCHHHCCCC	34.22	25521595
709	Phosphorylation	KSPAEAKSPAEVKSP CCHHHCCCCCCCCCC	36.07	25521595
715	Phosphorylation	KSPAEVKSPGEAKSP CCCCCCCCCCCCCCC	43.23	25521595
721	Phosphorylation	KSPGEAKSPAAVKSP CCCCCCCCCHHCCCH	27.15	25521595
727	Phosphorylation	KSPAAVKSPAEAKSP CCCHHCCCHHHCCCC	23.81	25521595
733	Phosphorylation	KSPAEAKSPAAVKSP CCHHHCCCCCCCCCC	27.15	25521595
739	Phosphorylation	KSPAAVKSPGEAKSP CCCCCCCCCCCCCCC	31.38	25521595
745	Phosphorylation	KSPGEAKSPGEAKSP CCCCCCCCCCCCCCH	45.22	25521595
751	Phosphorylation	KSPGEAKSPAEAKSP CCCCCCCCHHHCCCH	36.07	25521595
757	Phosphorylation	KSPAEAKSPAEAKSP CCHHHCCCHHHCCCC	36.07	25521595
763	Phosphorylation	KSPAEAKSPIEVKSP CCHHHCCCCCCCCCH	37.85	25521595
769	Phosphorylation	KSPIEVKSPEKAKTP CCCCCCCCHHHCCCC	43.39	25521595
775	Phosphorylation	KSPEKAKTPVKEGAK CCHHHCCCCCCCCCC	37.87	29899451
783	Phosphorylation	PVKEGAKSPAEAKSP CCCCCCCCHHHHCCH	28.89	25521595
789	Phosphorylation	KSPAEAKSPEKAKSP CCHHHHCCHHHCCCC	45.39	25521595
795	Phosphorylation	KSPEKAKSPVKEDIK CCHHHCCCCCCCCCC	39.52	22817900
809	Phosphorylation	KPPAEAKSPEKAKSP CCCHHCCCHHHCCCC	45.39	25521595
815	Phosphorylation	KSPEKAKSPVKEGAK CCHHHCCCCCCCCCC	39.52	22817900
834	Phosphorylation	AKPLDVKSPEAQTPV CCCCCCCCCCCCCCC	28.02	25521595
839	Phosphorylation	VKSPEAQTPVQEEAK CCCCCCCCCCCHHHC	32.26	25521595
849	Phosphorylation	QEEAKHPTDIRPPEQ CHHHCCCCCCCCHHH	43.72	24925903
859	Phosphorylation	RPPEQVKSPAKEKAK CCHHHCCCHHHHHCC	31.10	24925903
867	Phosphorylation	PAKEKAKSPEKEEAK HHHHHCCCCHHHHHH	43.05	22324799
888	Phosphorylation	PKKEEVKSPVKEEVK CCHHHCCCCHHHHHH	39.85	25521595
939	Phosphorylation	KVEEKKETPTEKPKD CCCCCCCCCCCCCCC	44.37	22817900
941	Phosphorylation	EEKKETPTEKPKDST CCCCCCCCCCCCCCH	65.15	22817900
947	Phosphorylation	PTEKPKDSTAEAKKE CCCCCCCCHHHHHHH	35.33	-
965	Phosphorylation	EKKKAVASEEETPAK HHHHHCCCCCCCHHH	38.65	25521595
988	Phosphorylation	PKEKTETTKTEAEDT CCCCCCCCCCHHHHH	29.72	20139300
1001	Phosphorylation	DTKAKEPSKPTETEK HHCCCCCCCCCCCCC	52.97	-
1050	Phosphorylation	KVKEDDKSLSKEPSK HCCCCCCCCCCCCCC	44.89	29899451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of NFH_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFH_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFH_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
NDEL1_MOUSE	Ndel1	physical	15208636

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFH_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-685 AND SER-691, ANDMASS SPECTROMETRY.	19367708 [Abstract]
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61 AND THR-499, AND MASSSPECTROMETRY.	17114649 [Abstract]
"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations."; Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.; Mol. Cell. Proteomics 5:914-922(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61; SER-416; SER-500;SER-523; SER-529; SER-535; SER-541; SER-547; SER-553; SER-559;SER-565; SER-571; SER-577; SER-583; SER-613; SER-619; SER-625;SER-631; SER-637; SER-649; SER-655; SER-661; SER-667; SER-673;SER-679; SER-685; SER-691; SER-697; SER-703; SER-709; SER-715;SER-721; SER-727; SER-733; SER-739; SER-745; SER-751; SER-757;SER-763; SER-769; THR-775; SER-783; SER-789; SER-815; SER-834;THR-839; SER-867 AND SER-888, AND MASS SPECTROMETRY.	16452087 [Abstract]