NF1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NF1_MOUSE
• UniProt AC: Q04690
• Protein Name: Neurofibromin
• Gene Name: Nf1
• Organism: Mus musculus (Mouse).
• Sequence Length: 2841
• Subcellular Localization: Nucleus. Nucleus, nucleolus.
• Protein Description: Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity..
• Protein Sequence: MAAHRPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDESNINKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKCLLLSMVKLIHADPKLLLCNPRKQGPETQSSTAELITGLVQLVPQSHMPEVAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHSLYLYGVGCEMSATGNTTQMSVDHDEFLRACTPGASLRKGRGNSSMDSTAGCSGTPPICRQAQTKLEVALYMFLWNPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNIEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSSSGLATYSPPMGAVSERKGSMISVMSSEGNIDSPVSRFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLSDSNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDIKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRVIITSSDWQHVSFEVDPTRLEPSESLEENQRNLLQMTEKFFHAIISSSSEFPSQLRSVCHCLYQATCHSLLNKATVKERKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKVLQSIANHVLFTKEEHMRPFNDFVKSNFDLARRFFLDIASDCPTSDAVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLLINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIESTVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRIFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKETQPWSSPKGSEGYLAATYPAVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRIPSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPALQSQLSITANLNLSNSMTSLATSQHSPGLDKENVELSPTAGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAHRPVEW ------CCCCCCHHH	24.23	-
30	Phosphorylation	IKTGQQNTHTKVSTE CCCCCCCCCCEECCC	26.95	25367039
32	Phosphorylation	TGQQNTHTKVSTEHN CCCCCCCCEECCCCC	30.97	25367039
35	Phosphorylation	QNTHTKVSTEHNKEC CCCCCEECCCCCCCE	29.63	25367039
36	Phosphorylation	NTHTKVSTEHNKECL CCCCEECCCCCCCEE	44.22	25367039
47	Phosphorylation	KECLINISKYKFSLV CCEEEEHHHHHHEEH	25.99	25367039
49	Phosphorylation	CLINISKYKFSLVIS EEEEHHHHHHEEHHH	15.52	-
139	Phosphorylation	AELRNSASGVLFSLS HHHHHCCCCEEEEEE	29.29	21183079
155	Phosphorylation	NNFNAVFSRISTRLQ CCHHHHHHHHHHHHH	23.31	21183079
158	Phosphorylation	NAVFSRISTRLQELT HHHHHHHHHHHHHHE	13.33	22817900
159	Phosphorylation	AVFSRISTRLQELTV HHHHHHHHHHHHHEE	32.78	22817900
302	Phosphorylation	NKKLFLDSLRKALAG CHHHHHHHHHHHHCC	32.02	26370283
362	Ubiquitination	NLLFNPSKPFSRGSQ HHCCCCCCCCCCCCC	51.87	27667366
465	Phosphorylation	PAIRMAPSLTFKEKV HHHHCCCCCCHHHHH	30.42	-
471	Acetylation	PSLTFKEKVTSLKFK CCCCHHHHHHCCCCC	51.94	15612465
476	Ubiquitination	KEKVTSLKFKEKPTD HHHHHCCCCCCCCCC	54.91	27667366
476	Acetylation	KEKVTSLKFKEKPTD HHHHHCCCCCCCCCC	54.91	15612477
480	Ubiquitination	TSLKFKEKPTDLETR HCCCCCCCCCCCHHH	54.53	27667366
486	Ubiquitination	EKPTDLETRSYKCLL CCCCCCHHHHHHHHH	32.01	27667366
490	Ubiquitination	DLETRSYKCLLLSMV CCHHHHHHHHHHHHH	21.47	27667366
624	Phosphorylation	ADRSSCHSLYLYGVG CCHHHCCCEEEECCC	24.04	22807455
626	Phosphorylation	RSSCHSLYLYGVGCE HHHCCCEEEECCCEE	10.82	21082442
655	Phosphorylation	DEFLRACTPGASLRK HHHHHHHCCCCCCCC	24.63	24719451
659	Phosphorylation	RACTPGASLRKGRGN HHHCCCCCCCCCCCC	34.32	22324799
667	Phosphorylation	LRKGRGNSSMDSTAG CCCCCCCCCCCCCCC	29.84	29899451
668	Phosphorylation	RKGRGNSSMDSTAGC CCCCCCCCCCCCCCC	30.52	29899451
672	Phosphorylation	GNSSMDSTAGCSGTP CCCCCCCCCCCCCCC	23.86	22807455
676	O-linked_Glycosylation	MDSTAGCSGTPPICR CCCCCCCCCCCCCHH	44.79	30016717
858	Phosphorylation	GVCLQQRSSSGLATY CCEEEECCCCCCCCC	25.23	26239621
859	Phosphorylation	VCLQQRSSSGLATYS CEEEECCCCCCCCCC	30.52	26239621
860	Phosphorylation	CLQQRSSSGLATYSP EEEECCCCCCCCCCC	38.79	24925903
864	Phosphorylation	RSSSGLATYSPPMGA CCCCCCCCCCCCCCC	29.58	24925903
865	Phosphorylation	SSSGLATYSPPMGAV CCCCCCCCCCCCCCC	17.36	24925903
866	Phosphorylation	SSGLATYSPPMGAVS CCCCCCCCCCCCCCC	20.41	25521595
873	Phosphorylation	SPPMGAVSERKGSMI CCCCCCCCCCCCCEE	31.02	24925903
878	Phosphorylation	AVSERKGSMISVMSS CCCCCCCCEEEEECC	18.91	25521595
881	Phosphorylation	ERKGSMISVMSSEGN CCCCCEEEEECCCCC	11.79	26160508
884	Phosphorylation	GSMISVMSSEGNIDS CCEEEEECCCCCCCC	24.07	26160508
885	Phosphorylation	SMISVMSSEGNIDSP CEEEEECCCCCCCCH	31.28	26160508
1142	Phosphorylation	GMSRRLASLRHCTVL HHHHHHHHHHHHHHH	30.44	24704852
1171	Phosphorylation	MHSIGLGYHKDLQTR CCCCCCCCCCCHHHH	15.67	-
1177	Phosphorylation	GYHKDLQTRATFMEV CCCCCHHHHHHHHHH	29.57	-
1377	Phosphorylation	LYQATCHSLLNKATV HHHHHHHHHHHHHHH	36.38	29899451
1403	Phosphorylation	SQRFPQNSIGAVGSA HHCCCCCCHHHHHHH	19.70	20139300
1409	Phosphorylation	NSIGAVGSAMFLRFI CCHHHHHHHHHHHHH	15.23	20139300
1540	Acetylation	VGRRPFDKMATLLAY CCCCCHHHHHHHHHH	30.71	7617809
1554	Acetylation	YLGPPEHKPVADTHW HHCCCCCCCCCCCCH	39.25	7617823
2030	Ubiquitination	GGLGSIKAEVMADTA CCCCCHHHHHHHHHH	16.97	27667366
2040	Ubiquitination	MADTAVALASGNVKL HHHHHHHHHCCCEEE	2.91	27667366
2051	Ubiquitination	NVKLVSSKVIGRMCK CEEEECHHHHHHHHH	30.30	27667366
2060	Ubiquitination	IGRMCKIIDKTCLSP HHHHHHHHHCCCCCC	2.44	27667366
2061	Ubiquitination	GRMCKIIDKTCLSPT HHHHHHHHCCCCCCC	43.62	27667366
2182	Phosphorylation	AAVIAFRSSYRDRSF EEEEEECCCCCCCCC	25.85	29899451
2183	Phosphorylation	AVIAFRSSYRDRSFS EEEEECCCCCCCCCC	21.67	23140645
2190	Phosphorylation	SYRDRSFSPGSYERE CCCCCCCCCCCHHHC	29.60	30372032
2193	Phosphorylation	DRSFSPGSYERETFA CCCCCCCCHHHCCEE	27.49	20415495
2462	Phosphorylation	CSLKHRKSLLLTDIS CCHHCCCHHEEEECC	24.98	22817900
2466	Phosphorylation	HRKSLLLTDISMENV CCCHHEEEECCCCCC	31.12	23984901
2469	Phosphorylation	SLLLTDISMENVPMD HHEEEECCCCCCCCC	23.89	22817900
2493	Phosphorylation	SYRTLKETQPWSSPK HHCCCCCCCCCCCCC	38.04	22817900
2497	Phosphorylation	LKETQPWSSPKGSEG CCCCCCCCCCCCCCC	43.37	29899451
2498	Phosphorylation	KETQPWSSPKGSEGY CCCCCCCCCCCCCCE	27.11	25521595
2502	Phosphorylation	PWSSPKGSEGYLAAT CCCCCCCCCCEEEEE	33.69	25619855
2505	Phosphorylation	SPKGSEGYLAATYPA CCCCCCCEEEEECCC	6.79	29514104
2509	Phosphorylation	SEGYLAATYPAVGQT CCCEEEEECCCCCCC	25.16	24925903
2510	Phosphorylation	EGYLAATYPAVGQTS CCEEEEECCCCCCCC	5.62	25619855
2516	Phosphorylation	TYPAVGQTSPRARKS ECCCCCCCCCCHHHH	34.47	24925903
2517	Phosphorylation	YPAVGQTSPRARKSM CCCCCCCCCCHHHHC	12.42	25521595
2523	Phosphorylation	TSPRARKSMSLDMGQ CCCCHHHHCCCCCCC	14.19	25521595
2525	Phosphorylation	PRARKSMSLDMGQPS CCHHHHCCCCCCCCC	28.45	25521595
2532	Phosphorylation	SLDMGQPSQANTKKL CCCCCCCCCCCHHHH	33.75	29899451
2545	Phosphorylation	KLLGTRKSFDHLISD HHHCCHHHHHHHHCC	32.39	25521595
2551	Phosphorylation	KSFDHLISDTKAPKR HHHHHHHCCCCCCCH	45.66	25159016
2563	Phosphorylation	PKRQEMESGITTPPK CCHHHHHCCCCCCHH	33.90	21183079
2566	Phosphorylation	QEMESGITTPPKMRR HHHHCCCCCCHHHHC	36.45	25521595
2567	Phosphorylation	EMESGITTPPKMRRV HHHCCCCCCHHHHCH	35.36	25521595
2588	Phosphorylation	METQRIPSSQQHPHL HCCCCCCCHHCCCCC	38.11	-
2599	Phosphorylation	HPHLRKVSVSESNVL CCCCEECCCCCCCCC	23.98	25521595
2601	Phosphorylation	HLRKVSVSESNVLLD CCEECCCCCCCCCCC	28.00	26239621
2603	Phosphorylation	RKVSVSESNVLLDEE EECCCCCCCCCCCHH	25.40	26239621
2613	Phosphorylation	LLDEEVLTDPKIQAL CCCHHHCCCHHHHHH	56.27	20415495
2804	Phosphorylation	DKENVELSPTAGHCN CHHCCCCCCCCCCCC	13.49	22324799
2806	Phosphorylation	ENVELSPTAGHCNSG HCCCCCCCCCCCCCC	41.73	20415495
2812	Phosphorylation	PTAGHCNSGRTRHGS CCCCCCCCCCCCCCC	34.74	23984901
2815	Phosphorylation	GHCNSGRTRHGSASQ CCCCCCCCCCCCHHH	30.46	23684622
2819	Phosphorylation	SGRTRHGSASQVQKQ CCCCCCCCHHHHHHH	20.72	26824392
2821	Phosphorylation	RTRHGSASQVQKQRS CCCCCCHHHHHHHHH	32.36	22817900
2828	Phosphorylation	SQVQKQRSAGSFKRN HHHHHHHHCCCCCHH	34.19	29899451
2831	Phosphorylation	QKQRSAGSFKRNSIK HHHHHCCCCCHHHCH	27.58	22817900
2836	Phosphorylation	AGSFKRNSIKKIV-- CCCCCHHHCHHCC--	39.56	29514104

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NF1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NF1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NF1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RPB1_MOUSE	Polr2a	physical	15999204
SMCA4_MOUSE	Smarca4	physical	15999204
ATF2_MOUSE	Atf2	physical	17700520
PO2F1_MOUSE	Pou2f1	physical	17700520
BRCA1_MOUSE	Brca1	physical	17700520

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2545 AND SER-2819, ANDMASS SPECTROMETRY.
PubMed: 19367708

"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations.";
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
Mol. Cell. Proteomics 6:283-293(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-2516, AND MASSSPECTROMETRY.
PubMed: 17114649