NEK4_HUMAN - dbPTM
NEK4_HUMAN - PTM Information in dbPTM
Basic Information of Protein
UniProt ID NEK4_HUMAN
UniProt AC P51957
Protein Name Serine/threonine-protein kinase Nek4
Gene Name NEK4
Organism Homo sapiens (Human).
Sequence Length 841
Subcellular Localization Cell projection, cilium . Cytoplasm .
Protein Description Protein kinase that seems to act exclusively upon threonine residues (By similarity). Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage..
Protein Sequence MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure		 ASA (%) Reference Orthologous
Protein Cluster
6Phosphorylation--MPLAAYCYLRVVG
--CCCEEEEEEEEEC		3.9724719451
8PhosphorylationMPLAAYCYLRVVGKG
CCCEEEEEEEEECCC		5.7229496907
10MethylationLAAYCYLRVVGKGSY
CEEEEEEEEECCCCC		8.46115484793
14UbiquitinationCYLRVVGKGSYGEVT
EEEEEECCCCCEEEE		32.83-
17PhosphorylationRVVGKGSYGEVTLVK
EEECCCCCEEEEEEE		26.4421964256
24UbiquitinationYGEVTLVKHRRDGKQ
CEEEEEEEECCCCCE		34.21-
30UbiquitinationVKHRRDGKQYVIKKL
EEECCCCCEEEEEEH		42.62-
43PhosphorylationKLNLRNASSRERRAA
EHHCCCCCHHHHHHH		33.21-
44PhosphorylationLNLRNASSRERRAAE
HHCCCCCHHHHHHHH		35.43-
58PhosphorylationEQEAQLLSQLKHPNI
HHHHHHHHHCCCCCE		41.9024719451
61UbiquitinationAQLLSQLKHPNIVTY
HHHHHHCCCCCEEEE		49.75-
133UbiquitinationHILHRDLKTQNVFLT
CCCCCCCCCCCEEEE		52.97-
146UbiquitinationLTRTNIIKVGDLGIA
EECCCEEEECHHHHH		36.05-
165PhosphorylationNHCDMASTLIGTPYY
HCCCHHHHHCCCCCC		16.92-
181UbiquitinationSPELFSNKPYNYKSD
CHHHHCCCCCCCCCH		47.91-
209UbiquitinationLKHAFNAKDMNSLVY
HHHHHCCCCHHHHHH		59.62-
216PhosphorylationKDMNSLVYRIIEGKL
CCHHHHHHHHHCCCC		11.03-
222UbiquitinationVYRIIEGKLPPMPRD
HHHHHCCCCCCCCCC		45.06-
243PhosphorylationELIRTMLSKRPEERP
HHHHHHHHCCCCCCC		18.79-
266PhosphorylationPYIKRQISFFLEATK
HHHHHHHHHHHHHHC		11.1223898821
273UbiquitinationSFFLEATKIKTSKNN
HHHHHHHCCCCCCCC		49.61-
273AcetylationSFFLEATKIKTSKNN
HHHHHHHCCCCCCCC		49.617298903
275AcetylationFLEATKIKTSKNNIK
HHHHHCCCCCCCCCC		48.587298911
278AcetylationATKIKTSKNNIKNGD
HHCCCCCCCCCCCCC		60.287298919
286PhosphorylationNNIKNGDSQSKPFAT
CCCCCCCCCCCCCEE		37.4418785766
289UbiquitinationKNGDSQSKPFATVVS
CCCCCCCCCCEEEEE		36.20-
319PhosphorylationSSEGSQTYIMGEGKC
CCCCCEEEEECCCEE		4.93-
335PhosphorylationSQEKPRASGLLKSPA
CCCCCCCCCCCCCHH		31.1129514088
339MethylationPRASGLLKSPASLKA
CCCCCCCCCHHHHCC		60.48115973887
339UbiquitinationPRASGLLKSPASLKA
CCCCCCCCCHHHHCC		60.48-
340PhosphorylationRASGLLKSPASLKAH
CCCCCCCCHHHHCCC		26.1822617229
343PhosphorylationGLLKSPASLKAHTCK
CCCCCHHHHCCCCCC		33.5122617229
345MethylationLKSPASLKAHTCKQD
CCCHHHHCCCCCCCC		35.31115973895
345UbiquitinationLKSPASLKAHTCKQD
CCCHHHHCCCCCCCC		35.31-
377PhosphorylationLPAKGRDSVSDGFVQ
ECCCCCCCCCCCCCC		23.3528985074
379PhosphorylationAKGRDSVSDGFVQEN
CCCCCCCCCCCCCCC		35.6529978859
442UbiquitinationGEKNEPVKPLQPLIK
CCCCCCCCCCHHHHH		51.21-
457PhosphorylationEQKPKDQSLALSPKL
CCCCCCCCCCCCCCC		26.2423401153
461PhosphorylationKDQSLALSPKLECSG
CCCCCCCCCCCCCCC		17.8723401153
467PhosphorylationLSPKLECSGTILAHS
CCCCCCCCCEEEECC		29.6030576142
469PhosphorylationPKLECSGTILAHSNL
CCCCCCCEEEECCCE		9.1227251275
474PhosphorylationSGTILAHSNLRLLGS
CCEEEECCCEEECCC		31.77-
481PhosphorylationSNLRLLGSSDSPASA
CCEEECCCCCCCHHH		30.8329255136
482PhosphorylationNLRLLGSSDSPASAS
CEEECCCCCCCHHHH		40.0729255136
484PhosphorylationRLLGSSDSPASASRV
EECCCCCCCHHHHHH		25.0629255136
487PhosphorylationGSSDSPASASRVAGI
CCCCCCHHHHHHHCH		30.1129255136
489PhosphorylationSDSPASASRVAGITG
CCCCHHHHHHHCHHC		25.4529255136
526PhosphorylationPDLQPHNSGSEPSLS
CCCCCCCCCCCCCHH		40.4328555341
533PhosphorylationSGSEPSLSRQRRQKR
CCCCCCHHHHHHHHH		30.6121712546
563PhosphorylationDLFAFQESPPRFLPS
HHHHHCCCCCCCCCC		29.4419664994
570PhosphorylationSPPRFLPSHPIVGKV
CCCCCCCCCCCEEEE		44.0023312004
576AcetylationPSHPIVGKVDVTSTQ
CCCCCEEEEECCCCH		25.1125953088
580PhosphorylationIVGKVDVTSTQKEAE
CEEEEECCCCHHHHH		22.9218669648
582PhosphorylationGKVDVTSTQKEAENQ
EEEECCCCHHHHHHC		34.0018669648
594PhosphorylationENQRRVVTGSVSSSR
HHCEEEEEECCCCCC		22.3830177828
596PhosphorylationQRRVVTGSVSSSRSS
CEEEEEECCCCCCCC		14.9530177828
598PhosphorylationRVVTGSVSSSRSSEM
EEEEECCCCCCCCCC		25.0530177828
599PhosphorylationVVTGSVSSSRSSEMS
EEEECCCCCCCCCCC		27.8430177828
600PhosphorylationVTGSVSSSRSSEMSS
EEECCCCCCCCCCCC		28.7730177828
602PhosphorylationGSVSSSRSSEMSSSK
ECCCCCCCCCCCCCC		32.3823909892
603PhosphorylationSVSSSRSSEMSSSKD
CCCCCCCCCCCCCCC		35.8929396449
606PhosphorylationSSRSSEMSSSKDRPL
CCCCCCCCCCCCCCC		27.6623909892
607PhosphorylationSRSSEMSSSKDRPLS
CCCCCCCCCCCCCCC		39.8523909892
608PhosphorylationRSSEMSSSKDRPLSA
CCCCCCCCCCCCCCH		31.1329396449
609UbiquitinationSSEMSSSKDRPLSAR
CCCCCCCCCCCCCHH		60.76-
622MethylationARERRRLKQSQEEMS
HHHHHHHHHHHHHHH		46.2024129315
622UbiquitinationARERRRLKQSQEEMS
HHHHHHHHHHHHHHH		46.20-
624PhosphorylationERRRLKQSQEEMSSS
HHHHHHHHHHHHHHC		37.6628348404
629PhosphorylationKQSQEEMSSSGPSVR
HHHHHHHHHCCCCHH		25.32-
630PhosphorylationQSQEEMSSSGPSVRK
HHHHHHHHCCCCHHE		37.69-
631PhosphorylationSQEEMSSSGPSVRKA
HHHHHHHCCCCHHEE		47.63-
634PhosphorylationEMSSSGPSVRKASLS
HHHHCCCCHHEEEEE		37.77-
637UbiquitinationSSGPSVRKASLSVAG
HCCCCHHEEEEEECC		39.92-
639PhosphorylationGPSVRKASLSVAGPG
CCCHHEEEEEECCCC		24.9823401153
641PhosphorylationSVRKASLSVAGPGKP
CHHEEEEEECCCCCC		13.8728450419
647UbiquitinationLSVAGPGKPQEEDQP
EEECCCCCCCCCCCC		46.81-
647AcetylationLSVAGPGKPQEEDQP
EEECCCCCCCCCCCC		46.8130590727
661PhosphorylationPLPARRLSSDCSVTQ
CCCCHHHCCCCCCCC		23.4827273156
662PhosphorylationLPARRLSSDCSVTQE
CCCHHHCCCCCCCCH		47.4229255136
665PhosphorylationRRLSSDCSVTQERKQ
HHHCCCCCCCCHHHH		32.9429255136
667PhosphorylationLSSDCSVTQERKQIH
HCCCCCCCCHHHHEE		14.3929978859
677PhosphorylationRKQIHCLSEDELSSS
HHHEEECCHHHHCCC		48.6929255136
682PhosphorylationCLSEDELSSSTSSTD
ECCHHHHCCCCCCCC		21.8629978859
684PhosphorylationSEDELSSSTSSTDKS
CHHHHCCCCCCCCCC		29.0023898821
690UbiquitinationSSTSSTDKSDGDYGE
CCCCCCCCCCCCCCC		51.00-
691PhosphorylationSTSSTDKSDGDYGEG
CCCCCCCCCCCCCCC		50.03-
699UbiquitinationDGDYGEGKGQTNEIN
CCCCCCCCCHHHHHH		43.47-
702PhosphorylationYGEGKGQTNEINALV
CCCCCCHHHHHHHHH		43.4122199227
721UbiquitinationQTLKLDSKESCEDVP
HCCCCCCCCCCCCCC		54.00-
723PhosphorylationLKLDSKESCEDVPVA
CCCCCCCCCCCCCCC		26.92-
737UbiquitinationANPVSEFKLHRKYRD
CCCCCCCCCCHHHCC		39.05-
745PhosphorylationLHRKYRDTLILHGKV
CCHHHCCEEEEECCC		13.4928555341
751UbiquitinationDTLILHGKVAEEAEE
CEEEEECCCHHHHHH		27.76-
762UbiquitinationEAEEIHFKELPSAIM
HHHHHCCCCCCCCCC		44.15-
766O-linked_GlycosylationIHFKELPSAIMPGSE
HCCCCCCCCCCCCHH		42.9528411811
772PhosphorylationPSAIMPGSEKIRRLV
CCCCCCCHHHHHHHH		29.8224719451
826PhosphorylationGEKYTTYSVKARQLK
CCCEEEEEEEHHHHH		18.3030631047
828UbiquitinationKYTTYSVKARQLKFF
CEEEEEEEHHHHHHH		31.73-
833UbiquitinationSVKARQLKFFEENMN
EEEHHHHHHHHHHCC		39.40-
Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources

Oops, there are no upstream regulatory protein records of NEK4_HUMAN !!
Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of NEK4_HUMAN !!
Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10) 		Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of NEK4_HUMAN !!
Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
TBA3C_HUMANTUBA3Cphysical
27173435
TBB1_HUMANTUBB1physical
27173435
FTM_HUMANRPGRIP1Lphysical
27173435
CTNA1_HUMANCTNNA1physical
27173435
Drug and Disease Associations
Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of NEK4_HUMAN

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Related Literatures of Post-Translational Modification
Phosphorylation
ReferencePubMed
"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-484, AND MASSSPECTROMETRY.
"Improved titanium dioxide enrichment of phosphopeptides from HeLacells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.";
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.;
J. Proteome Res. 6:4150-4162(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-563, AND MASSSPECTROMETRY.

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