dbPTM

MRCKB_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MRCKB_MOUSE
UniProt AC	Q7TT50
Protein Name	Serine/threonine-protein kinase MRCK beta
Gene Name	Cdc42bpb {ECO:0000312\|EMBL:AAP34402.1, ECO:0000312\|MGI:MGI:2136459}
Organism	Mus musculus (Mouse).
Sequence Length	1713
Subcellular Localization	Cytoplasm. Cell membrane Peripheral membrane protein Cytoplasmic side. Cell junction. Cell projection, lamellipodium . Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cel
Protein Description	Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A (By similarity). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation. [PubMed: 25107909]
Protein Sequence	MSAKVRLKKLEQLLLDGPWRNDSALSVETLLDVLVCLYTECSHSALRRDKYVAEFLEWAKPFTQLVKDMQLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFKKHAFFEGLNWENIRNLEAPYIPDVSSPSDTSNFDVDDDMLRNIEILPPGSHTGFSGLHLPFIGFTFTTESCFSDRGSLKSMTQSNTLTKDEDVQRDLENSLQIEAYERRIRRLEQEKLELSRKLQESTQTVQSLHGSTRALGNSNRDKEIKRLNEELERMKSKMADSNRLERQLEDTVTLRQEHEDSTHRLKGLEKQYRLARQEKEELHKQLVEASERLKSQTKELKDAHQQRKRALQEFSELNERMSELRSLKQKVSRQLRDKEEEMEVAMQKIDSMRQDLRKSEKSRKELEARLEDAAAEASKERKLREHSESFCKQMERELEALKVKQGGRGPGAASEHQQEISKIRSELEKKVLFYEEELVRREASHVLEVKNVKKEVHDSESHQLALQKEVLMLKDKLEKSKRERHSEMEEAIGTVKDKYERERAMLFDENKKLTAENEKLCSFVDKLTAQNRQLEDELQDLASKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELETLRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVQEELRKVKDSSLAFESKLKESEAKNRELLEEMQSLRKRMEEKFRADTGLKLPDFQDSIFEYFNTAPLAHDLTFRTSSASDQETQASKMDLSPSVSVATSTEQQEDMARPQQRPSPVPLPSTQALAMAGPKPKAHQFSIKSFPSPTQCSHCTSLMVGLIRQGYACEVCAFSCHVSCKDSAPQVCPIPPEQSKRPLGVDVQRGIGTAYKGYVKVPKPTGVKKGWQRAYAVVCDCKLFLYDLPEGKSTQPGVVASQVLDLRDEEFAVSSVLASDVIHATRRDIPCIFRVTASLLGSPSKTSSLLILTENENEKRKWVGILEGLQAILHKNRLKSQVVHVAQEAYDSSLPLIKAVLAAAIVDGDRIAVGLEEGLYVIELTRDVIVRAADCKKVYQIELAPKEKIAILLCGRNHHVHLYPWSSFDGAEASNFDIKLPETKGCQLIATGTLRKSSSTCLFVAVKRLILCYEIQRTKPFHRKFSELVAPGHVQWMAVFKDRLCVGYPSGFSLLSIQGDGPPLDLVNPTDPSLAFLSQQSFDALCAVELKSEEYLLCFSHMGLYVDPQGRRSRMQELMWPAAPVACSCSPTHVTVYSEYGVDVFDVRTMEWVQTIGLRRIRPLNSDGSLNLLGCEPPRLIYFKNKFSGTILNVPDTSDNSKKQMLRTRSKRRFVFKVPEEERLQQRREMLRDPELRSKMISNPTNFNHVAHMGPGDGMQVLMDLPLSAAPTVQEEKQGPTPAGLPRQPPSRSKPYVSWPSSGGSEPGVPVPLRSMSDPDQDFDKEPDSDSTKHSTPSNSSNPSGPPSPNSPHRSQLPMEGLDQPSCDA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
221	Phosphorylation	IRLADFGSCLKMNDD EEECCCCCCEEECCC	19.10	-
233	Phosphorylation	NDDGTVQSSVAVGTP CCCCCEEECEECCCC	23.57	-
239	Phosphorylation	QSSVAVGTPDYISPE EECEECCCCCCCCHH	13.34	-
314	Ubiquitination	TDVSEEAKDLIQRLI CCCCHHHHHHHHHHH	57.24	-
357	Phosphorylation	IRNLEAPYIPDVSSP HHCCCCCCCCCCCCC	31.94	29472430
362	Phosphorylation	APYIPDVSSPSDTSN CCCCCCCCCCCCCCC	43.86	29472430
363	Phosphorylation	PYIPDVSSPSDTSNF CCCCCCCCCCCCCCC	28.42	22817900
365	Phosphorylation	IPDVSSPSDTSNFDV CCCCCCCCCCCCCCC	56.31	23649490
367	Phosphorylation	DVSSPSDTSNFDVDD CCCCCCCCCCCCCCH	29.88	29472430
368	Phosphorylation	VSSPSDTSNFDVDDD CCCCCCCCCCCCCHH	40.25	29472430
414	Phosphorylation	SCFSDRGSLKSMTQS HHCCCCCCCCCHHCC	33.25	-
416	Ubiquitination	FSDRGSLKSMTQSNT CCCCCCCCCHHCCCC	39.62	27667366
421	Phosphorylation	SLKSMTQSNTLTKDE CCCCHHCCCCCCCCH	23.71	22817900
423	Phosphorylation	KSMTQSNTLTKDEDV CCHHCCCCCCCCHHH	40.84	23140645
425	Phosphorylation	MTQSNTLTKDEDVQR HHCCCCCCCCHHHHH	34.12	23140645
426	Ubiquitination	TQSNTLTKDEDVQRD HCCCCCCCCHHHHHH	63.33	27667366
464	Phosphorylation	LSRKLQESTQTVQSL HHHHHHHHHHHHHHH	16.92	23984901
465	Phosphorylation	SRKLQESTQTVQSLH HHHHHHHHHHHHHHH	27.75	23984901
467	Phosphorylation	KLQESTQTVQSLHGS HHHHHHHHHHHHHHH	22.17	23984901
470	Phosphorylation	ESTQTVQSLHGSTRA HHHHHHHHHHHHHHH	20.28	23984901
474	Phosphorylation	TVQSLHGSTRALGNS HHHHHHHHHHHCCCC	12.17	30352176
475	Phosphorylation	VQSLHGSTRALGNSN HHHHHHHHHHCCCCC	25.26	23984901
481	Phosphorylation	STRALGNSNRDKEIK HHHHCCCCCHHHHHH	31.29	26824392
500	Acetylation	ELERMKSKMADSNRL HHHHHHHHHHHCHHH	32.37	15616773
533	Malonylation	HRLKGLEKQYRLARQ HHHHHHHHHHHHHHH	58.64	26320211
614	Phosphorylation	VAMQKIDSMRQDLRK HHHHHHHHHHHHHHH	21.24	24719451
652	Phosphorylation	KLREHSESFCKQMER HHHHHHHHHHHHHHH	38.67	26824392
671	Methylation	LKVKQGGRGPGAASE HCCCCCCCCCCHHHH	55.76	-
677	Phosphorylation	GRGPGAASEHQQEIS CCCCCHHHHHHHHHH	34.77	26239621
684	Phosphorylation	SEHQQEISKIRSELE HHHHHHHHHHHHHHH	22.68	26239621
707	Phosphorylation	ELVRREASHVLEVKN HHHHHHHHHHEEEEC	14.91	-
749	Phosphorylation	KSKRERHSEMEEAIG HHHHHHHHHHHHHHH	44.88	21149613
757	Phosphorylation	EMEEAIGTVKDKYER HHHHHHHHHHHHHHH	20.47	21149613
789	Ubiquitination	KLCSFVDKLTAQNRQ HHHHHHHHHHHHHHH	42.30	-
840	Phosphorylation	GYLQALASKMTEELE HHHHHHHHHHHHHHH	24.66	-
843	Phosphorylation	QALASKMTEELETLR HHHHHHHHHHHHHHH	30.00	29899451
852	Phosphorylation	ELETLRSSSLGSRTL HHHHHHHCCCCCCCC	24.13	20139300
856	Phosphorylation	LRSSSLGSRTLDPLW HHHCCCCCCCCCHHH	27.64	29899451
858	Phosphorylation	SSSLGSRTLDPLWKV HCCCCCCCCCHHHHH	36.89	25521595
864	Ubiquitination	RTLDPLWKVRRSQKL CCCCHHHHHHHHCCC	33.28	-
868	Phosphorylation	PLWKVRRSQKLDMSA HHHHHHHHCCCCHHH	22.54	29514104
901	Methylation	QEELRKVKDSSLAFE HHHHHHHCCCHHHHH	55.73	-
910	Ubiquitination	SSLAFESKLKESEAK CHHHHHHHHHHHHHH	57.28	27667366
927	Phosphorylation	ELLEEMQSLRKRMEE HHHHHHHHHHHHHHH	28.91	22817900
954	Phosphorylation	FQDSIFEYFNTAPLA HHHHHHHHHHCCCCC	7.44	22817900
957	Phosphorylation	SIFEYFNTAPLAHDL HHHHHHHCCCCCCCC	21.54	26643407
965	Phosphorylation	APLAHDLTFRTSSAS CCCCCCCEECCCCCC	19.68	26643407
968	Phosphorylation	AHDLTFRTSSASDQE CCCCEECCCCCCHHH	23.89	27087446
969	Phosphorylation	HDLTFRTSSASDQET CCCEECCCCCCHHHH	21.46	29550500
970	Phosphorylation	DLTFRTSSASDQETQ CCEECCCCCCHHHHH	31.04	30352176
972	Phosphorylation	TFRTSSASDQETQAS EECCCCCCHHHHHHH	41.60	25521595
976	Phosphorylation	SSASDQETQASKMDL CCCCHHHHHHHHCCC	24.65	26643407
979	Phosphorylation	SDQETQASKMDLSPS CHHHHHHHHCCCCCC	20.78	26643407
984	Phosphorylation	QASKMDLSPSVSVAT HHHHCCCCCCEEECC	15.74	28066266
986	Phosphorylation	SKMDLSPSVSVATST HHCCCCCCEEECCCH	24.53	28066266
988	Phosphorylation	MDLSPSVSVATSTEQ CCCCCCEEECCCHHH	15.46	26060331
991	Phosphorylation	SPSVSVATSTEQQED CCCEEECCCHHHHHH	33.74	26060331
992	Phosphorylation	PSVSVATSTEQQEDM CCEEECCCHHHHHHH	21.87	26060331
993	Phosphorylation	SVSVATSTEQQEDMA CEEECCCHHHHHHHC	33.20	26060331
1007	Phosphorylation	ARPQQRPSPVPLPST CCCCCCCCCCCCCCH	40.49	26060331
1013	Phosphorylation	PSPVPLPSTQALAMA CCCCCCCCHHHHHHC	40.61	26060331
1014	Phosphorylation	SPVPLPSTQALAMAG CCCCCCCHHHHHHCC	18.87	26060331
1030	Phosphorylation	KPKAHQFSIKSFPSP CCCCEECCCCCCCCC	23.65	25338131
1182	Phosphorylation	CIFRVTASLLGSPSK EEEEEEHHHHCCCCC	18.11	28066266
1186	Phosphorylation	VTASLLGSPSKTSSL EEHHHHCCCCCCCEE	26.43	26824392
1188	Phosphorylation	ASLLGSPSKTSSLLI HHHHCCCCCCCEEEE	50.71	28066266
1190	Phosphorylation	LLGSPSKTSSLLILT HHCCCCCCCEEEEEE	28.08	21454597
1192	Phosphorylation	GSPSKTSSLLILTEN CCCCCCCEEEEEECC	32.21	25338131
1197	Phosphorylation	TSSLLILTENENEKR CCEEEEEECCCCCCC	29.75	21454597
1530	Ubiquitination	RLIYFKNKFSGTILN EEEEEECCCCCEEEC	41.31	-
1532	Phosphorylation	IYFKNKFSGTILNVP EEEECCCCCEEECCC	36.31	22871156
1534	Phosphorylation	FKNKFSGTILNVPDT EECCCCCEEECCCCC	22.54	22871156
1542	Phosphorylation	ILNVPDTSDNSKKQM EECCCCCCCCHHHHH	42.02	22871156
1635	Phosphorylation	GLPRQPPSRSKPYVS CCCCCCCCCCCCCEE	56.73	29514104
1637	Phosphorylation	PRQPPSRSKPYVSWP CCCCCCCCCCCEECC	42.17	26239621
1640	Phosphorylation	PPSRSKPYVSWPSSG CCCCCCCCEECCCCC	15.73	26239621
1642	Phosphorylation	SRSKPYVSWPSSGGS CCCCCCEECCCCCCC	27.68	26239621
1645	Phosphorylation	KPYVSWPSSGGSEPG CCCEECCCCCCCCCC	35.04	26060331
1646	Phosphorylation	PYVSWPSSGGSEPGV CCEECCCCCCCCCCC	42.99	22817900
1649	Phosphorylation	SWPSSGGSEPGVPVP ECCCCCCCCCCCCCC	44.11	26239621
1659	Phosphorylation	GVPVPLRSMSDPDQD CCCCCCCCCCCCCCC	30.26	23684622
1661	Phosphorylation	PVPLRSMSDPDQDFD CCCCCCCCCCCCCCC	47.43	30635358
1673	Phosphorylation	DFDKEPDSDSTKHST CCCCCCCCCCCCCCC	44.14	29472430
1675	Phosphorylation	DKEPDSDSTKHSTPS CCCCCCCCCCCCCCC	43.22	29472430
1676	Phosphorylation	KEPDSDSTKHSTPSN CCCCCCCCCCCCCCC	37.45	23684622
1679	Phosphorylation	DSDSTKHSTPSNSSN CCCCCCCCCCCCCCC	43.50	25619855
1680	Phosphorylation	SDSTKHSTPSNSSNP CCCCCCCCCCCCCCC	30.38	25619855
1682	Phosphorylation	STKHSTPSNSSNPSG CCCCCCCCCCCCCCC	49.81	25619855
1684	Phosphorylation	KHSTPSNSSNPSGPP CCCCCCCCCCCCCCC	35.51	25619855
1685	Phosphorylation	HSTPSNSSNPSGPPS CCCCCCCCCCCCCCC	57.94	25619855
1688	Phosphorylation	PSNSSNPSGPPSPNS CCCCCCCCCCCCCCC	69.08	27087446
1692	Phosphorylation	SNPSGPPSPNSPHRS CCCCCCCCCCCCCHH	39.59	27087446
1695	Phosphorylation	SGPPSPNSPHRSQLP CCCCCCCCCCHHCCC	26.01	27087446
1699	Phosphorylation	SPNSPHRSQLPMEGL CCCCCCHHCCCCCCC	32.79	23984901
1710	Phosphorylation	MEGLDQPSCDA---- CCCCCCCCCCC----	20.99	23984901

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1692	S	Phosphorylation	Kinase	MTOR	Q9JLN9	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of MRCKB_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MRCKB_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
CPNE1_HUMAN	CPNE1	physical	12522145
CPNE4_HUMAN	CPNE4	physical	12522145

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MRCKB_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1692, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1692 AND SER-1695, ANDMASS SPECTROMETRY.	17242355 [Abstract]
"Immunoaffinity profiling of tyrosine phosphorylation in cancercells."; Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,Zha X.-M., Polakiewicz R.D., Comb M.J.; Nat. Biotechnol. 23:94-101(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-954, AND MASSSPECTROMETRY.	15592455 [Abstract]