MPIP2_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MPIP2_RAT
• UniProt AC: P48966
• Protein Name: M-phase inducer phosphatase 2
• Gene Name: Cdc25b
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 574
• Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .
• Protein Description: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity)..
• Protein Sequence: MEVPPQKSAPGSALSTARVLGGIQRPRHLSGFGFGSDGLLGSPERAASSSPVTTLTQTMYNLAGLGSETPKTQVGSLSFQNRLTDLSLSRRTSECSLSSESSESSDAGLCMDSPSPMDPQTAERTFEQAIQAASRVIQKMQFTIKASVFASEAAGHSPVLQNITNSQALDSWEKDEAGYRAASSPGEDKENDGYIFKMPQKLPHSSSARALAEWASRREAFTQRPSSAPDLMCLTTDGKMDVEEASPVAQSSSLTPVERACEEDDGFVDILESDLKDDDMVPAGMENLISAPLVKKLDKEEEQDLIMFSKCQRLFRSPSMPCSVIRPILKRLERPHDRDVPVLSKRRKSGTPLEEQQLEEPKARVFRSKSLCHEIESILDSDHRGLIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVEKFVIVDCRYPYEYEGGHIKNAVNLPLEPDAETFLLKHPITPCNLDKRIILIFHCEFSSERGPRMCRFIRERDRAANDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHAAFRDELRNFRLKTRSWAGERSTTQLCSRLQDQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
42	Phosphorylation	GSDGLLGSPERAASS CCCCCCCCHHHHHCC	24.45	27097102
166	Phosphorylation	VLQNITNSQALDSWE CCCCCCCCCHHHHHH	14.26	-
246	Phosphorylation	KMDVEEASPVAQSSS CCCHHHHCCCCCCCC	24.16	-
319	Phosphorylation	QRLFRSPSMPCSVIR HHHHCCCCCCHHHHH	36.28	-
349	Phosphorylation	VLSKRRKSGTPLEEQ CHHCCCCCCCCCHHH	46.19	28432305
351	Phosphorylation	SKRRKSGTPLEEQQL HCCCCCCCCCHHHHC	31.62	28432305
370	Phosphorylation	ARVFRSKSLCHEIES HHHHHCHHHHHHHHH	37.16	23984901
557	Phosphorylation	NFRLKTRSWAGERST HCCCCCCCCCCCCHH	26.89	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
166	S	Phosphorylation	Kinase	MELK	-	Uniprot
319	S	Phosphorylation	Kinase	MAPK14	P70618	Uniprot
319	S	Phosphorylation	Kinase	MELK	-	Uniprot
349	S	Phosphorylation	Kinase	AURKA	P59241	Uniprot
370	S	Phosphorylation	Kinase	BRSK1	B2DD29	Uniprot
370	S	Phosphorylation	Kinase	MAPK14	P70618	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
166	S	Phosphorylation	Phosphorylation by MELK at Ser-166 promotes localization to the centrosome and the spindle poles during mitosis.	-
319	S	Phosphorylation	Phosphorylation at Ser-319 and Ser-370 by MAPK14 is required for binding to 14-3-3 proteins (By similarity).	-
349	S	Phosphorylation	Phosphorylation at Ser-349 by AURKA might locally participate in the control of the onset of mitosis.	-
370	S	Phosphorylation	Phosphorylation at Ser-319 and Ser-370 by MAPK14 is required for binding to 14-3-3 proteins (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MPIP2_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CDK5_RAT	Cdk5	physical	22899714
1433E_RAT	Ywhae	physical	22899714

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.