MPIP2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MPIP2_MOUSE
• UniProt AC: P30306
• Protein Name: M-phase inducer phosphatase 2
• Gene Name: Cdc25b
• Organism: Mus musculus (Mouse).
• Sequence Length: 576
• Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .
• Protein Description: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity)..
• Protein Sequence: MEVPLQKSAPGSALSPARVLGGIQRPRHLSVFEFESDGFLGSPEPTASSSPVTTLTQTMHNLAGLGSEPPKAQVGSLSFQNRLADLSLSRRTSECSLSSESSESSDAGLCMDSPSPVDPQMAERTFEQAIQAASRVIQNEQFTIKRFRSLPVRLLEHSPVLQSITNSRALDSWRKTEAGYRAAANSPGEDKENDGYIFKMPQELPHSSSAQALAEWVSRRQAFTQRPSSAPDLMCLTTEWKMEVEELSPVAQSSSLTPVERASEEDDGFVDILESDLKDDEKVPAGMENLISAPLVKKLDKEEEQDLIMFSKCQRLFRSPSMPCSVIRPILKRLERPQDRDVPVQSKRRKSVTPLEEQQLEEPKARVFRSKSLCHEIENILDSDHRGLIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVEKFVIVDCRYPYEYEGGHIKNAVNLPLERDAETFLLQRPIMPCSLDKRIILIFHCEFSSERGPRMCRFIRERDRAANDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFRDELRNFRLKTRSWAGERSRRELCSRLQDQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8	Phosphorylation	MEVPLQKSAPGSALS CCCCCCCCCCCCCCC	27.08	25777480
12	Phosphorylation	LQKSAPGSALSPARV CCCCCCCCCCCHHHH	25.61	25777480
15	Phosphorylation	SAPGSALSPARVLGG CCCCCCCCHHHHCCC	19.07	24453211
42	Phosphorylation	ESDGFLGSPEPTASS ECCCCCCCCCCCCCC	28.39	-
78	Phosphorylation	KAQVGSLSFQNRLAD CCCCCCCCHHHHHHH	26.87	25338131
87	Phosphorylation	QNRLADLSLSRRTSE HHHHHHHCCCCCCCC	25.50	28066266
89	Phosphorylation	RLADLSLSRRTSECS HHHHHCCCCCCCCCC	19.15	29514104
149	Phosphorylation	FTIKRFRSLPVRLLE CCHHCHHCCCHHHHC	34.02	22348958
158	Phosphorylation	PVRLLEHSPVLQSIT CHHHHCCCHHHHHHC	13.82	26643407
167	Phosphorylation	VLQSITNSRALDSWR HHHHHCCCCHHHHHH	15.41	-
186	Phosphorylation	GYRAAANSPGEDKEN HHHHHCCCCCCCCCC	29.56	27566939
229	Phosphorylation	AFTQRPSSAPDLMCL HHHCCCCCCCCCEEE	46.64	18633139
248	Phosphorylation	KMEVEELSPVAQSSS EEEHHHHCCCCCCCC	22.17	25777480
319	Phosphorylation	KCQRLFRSPSMPCSV HHHHHHCCCCCCHHH	17.62	23608596
321	Phosphorylation	QRLFRSPSMPCSVIR HHHHCCCCCCHHHHH	36.28	22348958
351	Phosphorylation	VQSKRRKSVTPLEEQ CCCCCCCCCCCHHHH	29.43	21082442
353	Phosphorylation	SKRRKSVTPLEEQQL CCCCCCCCCHHHHHC	29.28	27600695
370	Phosphorylation	PKARVFRSKSLCHEI HHHHHHHCHHHHHHH	18.35	25777480
372	Phosphorylation	ARVFRSKSLCHEIEN HHHHHCHHHHHHHHH	37.16	26824392
559	Phosphorylation	NFRLKTRSWAGERSR HHHHHHCCCCCHHHH	26.89	25266776

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
149	S	Phosphorylation	Kinase	PKACA	P17612	PSP
149	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
167	S	Phosphorylation	Kinase	MELK	Q61846	Uniprot
229	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
321	S	Phosphorylation	Kinase	PKACA	P17612	PSP
321	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
321	S	Phosphorylation	Kinase	MELK	Q61846	Uniprot
321	S	Phosphorylation	Kinase	MAPK14	P47811	Uniprot
321	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
351	S	Phosphorylation	Kinase	AKT1	P31750	PSP
351	S	Phosphorylation	Kinase	AURA	P97477	PSP
372	S	Phosphorylation	Kinase	BRSK1	Q5RJI5	Uniprot
372	S	Phosphorylation	Kinase	MAPK14	P47811	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
167	S	Phosphorylation	Phosphorylation by MELK at Ser-167 promotes localization to the centrosome and the spindle poles during mitosis.	-
321	S	Phosphorylation	Phosphorylation at Ser-321 and Ser-372 by MAPK14 is required for binding to 14-3-3 proteins (By similarity).	-
351	S	Phosphorylation	Phosphorylation at Ser-351 by AURKA might locally participate in the control of the onset of mitosis.	-
372	S	Phosphorylation	Phosphorylation at Ser-321 and Ser-372 by MAPK14 is required for binding to 14-3-3 proteins (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MPIP2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MPIP2_HUMAN	CDC25B	physical	20360068

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.