MPIP1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MPIP1_RAT
• UniProt AC: P48965
• Protein Name: M-phase inducer phosphatase 1
• Gene Name: Cdc25a
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 525
• Protein Description: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro (By similarity). Phosphorylation by PIM1 leads to an increase in phosphatase activity..
• Protein Sequence: MELGPEPPHRRRLLFTCSPTPAPQPTGKVQFGASRAGGLSPVTNLTVTMDQLEGLGSDYEKPMDVRNSSSLQRMGSSESTDSGFCLDSPGPLDSKENLEISLRRINCLPQKLLGCSPALKRSHSDSLDHDIFQLIDQDENKENEAFEFKKPIRPASRGCLNAHVHEESKDPFTHRQNSAPARMLSSNESDISESGNFSPLFTPQSPVKASLSDEDDGFIDLLDGENLKNDEETPSCMSSLWTAPLVMRRPTNLADRCGLFDSPSPCSSTSSCSTRAVKRADRSHEESPRGTKRRKSSEASPVKADVPEPTQLPHQSLSLTSFPKGTIENIFHSDPRDLIGDFSKGYLFHTVSGKHQDLKYISPEIMASVLNGKFANLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEEFLLKKPIVPADGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
76	Phosphorylation	SSLQRMGSSESTDSG HHHHCCCCCCCCCCC	22.91	-
79	Phosphorylation	QRMGSSESTDSGFCL HCCCCCCCCCCCCCC	39.19	-
82	Phosphorylation	GSSESTDSGFCLDSP CCCCCCCCCCCCCCC	33.58	-
88	Phosphorylation	DSGFCLDSPGPLDSK CCCCCCCCCCCCCCH	20.86	-
124	Phosphorylation	PALKRSHSDSLDHDI HHHHCCCCCCCCHHH	30.09	-
178	Phosphorylation	PFTHRQNSAPARMLS CCCCCCCCCCCHHHC	27.56	-
283	Phosphorylation	AVKRADRSHEESPRG HHHHHHCCCCCCCCC	35.48	-
296	Phosphorylation	RGTKRRKSSEASPVK CCCCCCCCCCCCCCC	31.44	-
297	Phosphorylation	GTKRRKSSEASPVKA CCCCCCCCCCCCCCC	39.97	-
445	Phosphorylation	RGPRMCRYVRERDRL CCCCHHHHHHHHHCC	10.03	24972320
508	Phosphorylation	KFRTKSRTWAGEKSK HHHHHCCCCCCHHHH	27.10	-
514	Phosphorylation	RTWAGEKSKREMYSR CCCCCHHHHHHHHHH	32.42	-
520	Phosphorylation	KSKREMYSRLKKL-- HHHHHHHHHHHCC--	29.81	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
76	S	Phosphorylation	Kinase	CHEK1	Q91ZN7	Uniprot
79	S	Phosphorylation	Kinase	NEK11	-	Uniprot
82	S	Phosphorylation	Kinase	NEK11	-	Uniprot
88	S	Phosphorylation	Kinase	NEK11	-	Uniprot
124	S	Phosphorylation	Kinase	CHEK1	Q91ZN7	Uniprot
124	S	Phosphorylation	Kinase	CHEK2	-	Uniprot
178	S	Phosphorylation	Kinase	CHEK1	Q91ZN7	Uniprot
283	S	Phosphorylation	Kinase	CHEK1	Q91ZN7	Uniprot
283	S	Phosphorylation	Kinase	CHEK2	-	Uniprot
296	S	Phosphorylation	Kinase	CHEK1	Q91ZN7	Uniprot
296	S	Phosphorylation	Kinase	CHEK2	-	Uniprot
508	T	Phosphorylation	Kinase	CHEK1	Q91ZN7	Uniprot
514	S	Phosphorylation	Kinase	PLK3	Q9R011	Uniprot
520	S	Phosphorylation	Kinase	PLK3	Q9R011	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
76	S	ubiquitylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
76	S	Phosphorylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
76	S	Phosphorylation	Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11.	-
76	S	Phosphorylation	Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-283, Ser-296 and Thr-508 during checkpoint mediated cell cycle arrest.	-
79	S	Phosphorylation	Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11.	-
82	S	Phosphorylation	Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11.	-
88	S	Phosphorylation	Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11.	-
124	S	Phosphorylation	Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-283, Ser-296 and Thr-508 during checkpoint mediated cell cycle arrest.	-
124	S	Phosphorylation	Also phosphorylated by CHEK2 on Ser-124, Ser-283, and Ser-296 during checkpoint mediated cell cycle arrest.	-
124	S	ubiquitylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
124	S	Phosphorylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
178	S	ubiquitylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
178	S	Phosphorylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
178	S	Phosphorylation	Phosphorylation on Ser-178 and Thr-508 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A.	-
178	S	Phosphorylation	Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-283, Ser-296 and Thr-508 during checkpoint mediated cell cycle arrest.	-
283	S	Phosphorylation	Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-283, Ser-296 and Thr-508 during checkpoint mediated cell cycle arrest.	-
283	S	Phosphorylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
283	S	ubiquitylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
283	S	Phosphorylation	Also phosphorylated by CHEK2 on Ser-124, Ser-283, and Ser-296 during checkpoint mediated cell cycle arrest.	-
296	S	Phosphorylation	Also phosphorylated by CHEK2 on Ser-124, Ser-283, and Ser-296 during checkpoint mediated cell cycle arrest.	-
296	S	ubiquitylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
296	S	Phosphorylation	Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-283 and Ser-296 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex.	-
296	S	Phosphorylation	Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-283, Ser-296 and Thr-508 during checkpoint mediated cell cycle arrest.	-
508	T	Phosphorylation	Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-283, Ser-296 and Thr-508 during checkpoint mediated cell cycle arrest.	-
508	T	Phosphorylation	Phosphorylation on Ser-178 and Thr-508 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MPIP1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CDK5_RAT	Cdk5	physical	22899714
1433E_RAT	Ywhae	physical	22899714

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.