MP2K7_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MP2K7_MOUSE
• UniProt AC: Q8CE90
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• Gene Name: Map2k7 {ECO:0000312|MGI:MGI:1346871}
• Organism: Mus musculus (Mouse).
• Sequence Length: 535
• Subcellular Localization: Nucleus . Cytoplasm .
• Protein Description: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis..
• Protein Sequence: MAASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPRPIIVITLSPAPAPSQRAALQLPLANDGGSRSPSSESSPQHPTPPTRPRHMLGLPSTLFTPRSMESIEIDQKLQEIMKQTGYLTIGGQRYQAEINDLENLGEMGSGTCGQVWKMRFRKTGHIIAVKQMRRSGNKEENKRILMDLDVVLKSHDCPYIVQCFGTFITNTDVFIAMELMGTCAEKLKKRMQGPIPERILGKMTVAIVKALYYLKEKHGVIHRDVKPSNILLDERGQIKLCDFGISGRLVDSKAKTRSAGCAAYMAPERIDPPDPTKPDYDIRADVWSLGISLVELATGQFPYKNCKTDFEVLTKVLQEEPPLLPGHMGFSGDFQSFVKDCLTKDHRKRPKYNKLLEHSFIKHYEILEVDVASWFKDVMAKTESPRTSGVLSQHHLPFFSTSVTWGAWPLAAQTPFQSGVIRCRGRVPSPRRATGGSGGQPCVCAGGPGPSFTEMGPSPSPMLSNTFFTPDPGACPGASTWGLPRRRLCQLLTTSTPGCC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAASSLEQK ------CCCHHHHHH	18.95	-
9	Ubiquitination	AASSLEQKLSRLEAK CCHHHHHHHHHHHHH	38.77	22790023
9 (in isoform 6)	Ubiquitination	-	38.77	22790023
35 (in isoform 8)	Phosphorylation	-	18.04	26824392
35 (in isoform 2)	Phosphorylation	-	18.04	26824392
35	Phosphorylation	IDLNLDISPQRPRPI CCCCCCCCCCCCCCE	18.04	28066266
42 (in isoform 8)	Phosphorylation	-	4.08	26643407
42 (in isoform 2)	Phosphorylation	-	4.08	26643407
53 (in isoform 2)	Phosphorylation	-	29.90	25338131
53 (in isoform 8)	Phosphorylation	-	29.90	25338131
55 (in isoform 2)	Phosphorylation	-	37.66	25338131
55 (in isoform 8)	Phosphorylation	-	37.66	25338131
60 (in isoform 2)	Phosphorylation	-	35.99	25338131
60 (in isoform 8)	Phosphorylation	-	35.99	25338131
66	Phosphorylation	LQLPLANDGGSRSPS HCCCCCCCCCCCCCC	58.17	-
69	Phosphorylation	PLANDGGSRSPSSES CCCCCCCCCCCCCCC	34.80	25338131
71	Phosphorylation	ANDGGSRSPSSESSP CCCCCCCCCCCCCCC	30.72	29472430
73	Phosphorylation	DGGSRSPSSESSPQH CCCCCCCCCCCCCCC	48.24	26643407
74	Phosphorylation	GGSRSPSSESSPQHP CCCCCCCCCCCCCCC	44.90	26643407
76	Phosphorylation	SRSPSSESSPQHPTP CCCCCCCCCCCCCCC	49.63	26643407
77	Phosphorylation	RSPSSESSPQHPTPP CCCCCCCCCCCCCCC	25.24	26643407
82	Phosphorylation	ESSPQHPTPPTRPRH CCCCCCCCCCCCCCH	39.51	29899451
83	Phosphorylation	SSPQHPTPPTRPRHM CCCCCCCCCCCCCHH	32.49	-
85	Phosphorylation	PQHPTPPTRPRHMLG CCCCCCCCCCCHHCC	55.28	26643407
99	Phosphorylation	GLPSTLFTPRSMESI CCCCCCCCCCCHHHC	22.39	-
102	Phosphorylation	STLFTPRSMESIEID CCCCCCCCHHHCCHH	28.27	29899451
105	Phosphorylation	FTPRSMESIEIDQKL CCCCCHHHCCHHHHH	19.51	29899451
281	Phosphorylation	KLCDFGISGRLVDSK EECCCCCCCEECCCC	20.45	28066266
287	Phosphorylation	ISGRLVDSKAKTRSA CCCEECCCCCCCCCC	27.34	26643407
291	Phosphorylation	LVDSKAKTRSAGCAA ECCCCCCCCCCCCCH	34.41	18032450
293	Phosphorylation	DSKAKTRSAGCAAYM CCCCCCCCCCCCHHC	33.96	24759943
328 (in isoform 4)	Phosphorylation	-	3.07	23984901
330 (in isoform 4)	Phosphorylation	-	37.67	26239621
333 (in isoform 4)	Phosphorylation	-	44.11	23984901
334 (in isoform 4)	Phosphorylation	-	22.86	23984901
338 (in isoform 4)	Phosphorylation	-	19.00	29899451
342 (in isoform 6)	Ubiquitination	-	61.60	22790023
342	Ubiquitination	QFPYKNCKTDFEVLT CCCCCCCCCHHHHHH	61.60	22790023
361 (in isoform 3)	Phosphorylation	-	24.31	25168779
365 (in isoform 3)	Phosphorylation	-	5.99	26643407
373 (in isoform 5)	Phosphorylation	-	6.56	23984901
375 (in isoform 5)	Phosphorylation	-	27.62	26239621
377 (in isoform 3)	Phosphorylation	-	10.79	25168779
378 (in isoform 5)	Phosphorylation	-	40.53	23984901
379 (in isoform 5)	Phosphorylation	-	38.29	23984901
383 (in isoform 5)	Phosphorylation	-	70.41	29899451
401 (in isoform 2)	Phosphorylation	-	2.91	23984901
403	Phosphorylation	IKHYEILEVDVASWF HCCEEEEEEEHHHHH	42.19	24719451
403 (in isoform 2)	Phosphorylation	-	42.19	26239621
406 (in isoform 2)	Phosphorylation	-	5.70	23984901
407 (in isoform 2)	Phosphorylation	-	9.15	23984901
411 (in isoform 2)	Phosphorylation	-	43.06	29899451
417 (in isoform 6)	Phosphorylation	-	31.96	23984901
419 (in isoform 6)	Phosphorylation	-	25.55	26239621
419	Phosphorylation	DVMAKTESPRTSGVL HHHHHCCCCCCCCCC	25.55	26824392
422 (in isoform 6)	Phosphorylation	-	32.96	23984901
423 (in isoform 6)	Phosphorylation	-	28.34	23984901
427 (in isoform 6)	Phosphorylation	-	25.68	29899451
427	Phosphorylation	PRTSGVLSQHHLPFF CCCCCCCCCCCCCCE	25.68	-
434 (in isoform 8)	Phosphorylation	-	8.01	25168779
438 (in isoform 8)	Phosphorylation	-	5.45	26643407
450 (in isoform 8)	Phosphorylation	-	17.13	25168779
450 (in isoform 7)	Phosphorylation	-	17.13	25168779
454 (in isoform 7)	Phosphorylation	-	14.21	26643407
464	Phosphorylation	RCRGRVPSPRRATGG EECCCCCCCCCCCCC	28.34	19186949
466 (in isoform 7)	Phosphorylation	-	45.24	25168779

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
287	S	Phosphorylation	Kinase	MAP3K	-	Uniprot
291	T	Phosphorylation	Kinase	MAP3K	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
287	S	Phosphorylation	Activated by phosphorylation on Ser-287 and Thr-291 by MAP kinase kinase kinases (MAP3Ks).	9891090
291	T	Phosphorylation	Activated by phosphorylation on Ser-287 and Thr-291 by MAP kinase kinase kinases (MAP3Ks).	9891090

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MP2K7_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
JIP3_MOUSE	Mapk8ip3	physical	10629060
MK10_MOUSE	Mapk10	physical	15218018
JIP3_MOUSE	Mapk8ip3	physical	15767678

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.