dbPTM

MK10_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MK10_MOUSE
UniProt AC	Q61831
Protein Name	Mitogen-activated protein kinase 10
Gene Name	Mapk10
Organism	Mus musculus (Mouse).
Sequence Length	464
Subcellular Localization	Cytoplasm . Membrane Lipid-anchor . Nucleus . Mitochondrion . Palmitoylation regulates MAPK10 trafficking to cytoskeleton (By similarity). Recruited to the mitochondria in the presence of SARM1..
Protein Description	Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock. [PubMed: 22441692]
Protein Sequence	MSLHFLYYCSEPTLDVKIAFCQGFDKHVDVSSIAKHYNMSKSKVDNQFYSVEVGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAVLDRNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRSYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPVKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWKELIYKEVMNSEEKTKNGVVKSQPSPSGAAVNSSESLPPSSAVNDISSMSTDQTLASDTDSSLEASAGPLGCCR

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
167	Phosphorylation	ELDHERMSYLLYQML CCCHHHHHHHHHHHH	20.89	16483929
191	Ubiquitination	GIIHRDLKPSNIVVK CEECCCCCHHHEEEE	51.17	-
204	Ubiquitination	VKSDCTLKILDFGLA EECCCEEEECCHHCC	24.06	-
213	Phosphorylation	LDFGLARTAGTSFMM CCHHCCCCCCCCCCC	24.67	22499769
216	Phosphorylation	GLARTAGTSFMMTPY HCCCCCCCCCCCCCH	19.14	22499769
217	Phosphorylation	LARTAGTSFMMTPYV CCCCCCCCCCCCCHH	15.38	22499769
221	Phosphorylation	AGTSFMMTPYVVTRY CCCCCCCCCHHEEEH	10.32	25521595
223	Phosphorylation	TSFMMTPYVVTRYYR CCCCCCCHHEEEHHC	9.82	25521595
226	Phosphorylation	MMTPYVVTRYYRAPE CCCCHHEEEHHCCCH	11.69	22322096
311	Ubiquitination	YAGLTFPKLFPDSLF CCCCCCCCCCCCCCC	59.58	-
346	Acetylation	MLVIDPVKRISVDDA HCCCCCCCCCCHHHH	49.77	129953
396	Ubiquitination	EWKELIYKEVMNSEE HHHHHHHHHHHCCHH	36.52	-
462	S-palmitoylation	ASAGPLGCCR----- HHCCCCCCCC-----	2.02	-
463	S-palmitoylation	SAGPLGCCR------ HCCCCCCCC------	5.70	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
221	T	Phosphorylation	Kinase	MAP2K7	Q8CE90	Uniprot
223	Y	Phosphorylation	Kinase	MAP2K4	P47809	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
221	T	Phosphorylation	-
Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme.
221	T	Phosphorylation	-
MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MK10_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
MABP1_MOUSE	Mapkbp1	physical	10471813

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MK10_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-221 AND TYR-223, ANDMASS SPECTROMETRY.	18034455 [Abstract]

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