MLP3B_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MLP3B_MOUSE
• UniProt AC: Q9CQV6
• Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• Gene Name: Map1lc3b
• Organism: Mus musculus (Mouse).
• Sequence Length: 125
• Subcellular Localization: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome . LC3-II binds to the autophagic membranes (By similarity). Localizes also to discrete punctae a
• Protein Description: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway..
• Protein Sequence: MPSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDEDGFLYMVYASQETFGTAMAV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
12	Phosphorylation	KTFKQRRSFEQRVED HHHHHHHCHHHHHHH	35.01	29176673
30	Ubiquitination	IREQHPTKIPVIIER HHHHCCCCCCEEEEE	49.84	27667366
49	Ubiquitination	KQLPVLDKTKFLVPD CCCCCCCCCCCCCCC	49.83	-
50	Phosphorylation	QLPVLDKTKFLVPDH CCCCCCCCCCCCCCC	27.05	-
51	Ubiquitination	LPVLDKTKFLVPDHV CCCCCCCCCCCCCCC	42.30	27667366
65	Ubiquitination	VNMSELIKIIRRRLQ CCHHHHHHHHHHHHC	44.71	-
120	Phosphatidylethanolamine amidation	YASQETFGTAMAV-- EECCCCCCCCCCC--	21.71	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
12	S	Phosphorylation	Kinase	PKA	-	Uniprot
50	T	Phosphorylation	Kinase	MST2	Q13188	PSP
50	T	Phosphorylation	Kinase	MST1	Q13043	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
12	S	Phosphorylation	Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MLP3B_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SRC_MOUSE	Src	physical	22138575
CBL_MOUSE	Cbl	physical	22138575
ATG10_MOUSE	Atg10	physical	12890687

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.