MK06_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MK06_MOUSE
• UniProt AC: Q61532
• Protein Name: Mitogen-activated protein kinase 6
• Gene Name: Mapk6
• Organism: Mus musculus (Mouse).
• Sequence Length: 720
• Subcellular Localization: Cytoplasm. Nucleus. Translocates to the cytoplasm following interaction with MAPKAPK5.
• Protein Description: Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle..
• Protein Sequence: MAEKFESLMNIHGFDLGSRYMDLKPLGCGGNGLVFSAVDNDCDKRVAIKKIVLTDPQSVKHALREIKIIRRLDHDNIVKVFEILGPSGSQLTDDVGSLTELNSVYIVQEYMETDLANVLEQGPLLEEHARLFMYQLLRGLKYIHSANVLHRDLKPANLFINTEDLVLKIGDFGLARIMDPHYSHKGHLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCIFAEMLTGKTLFAGAHELEQMQLILDSIPVVHEEDRQELLSVIPVYIRNDMTEPHRPLTQLLPGISREALDFLEQILTFSPMDRLTAEEALSHPYMSIYSFPTDEPISSHPFHIEDEVDDILLMDETHSHIYNWERYHDCQFSEHDWPIHNNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTSYSAEPCWQYPDHHENKYCDLECSHTCNYKTRSSPYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKEKSDKRGKSKCERNGLVKAQIALEEASQQLAERERGQGFDFDSFIAGTIQLSAQHQSADVVDKLNDLNSSVSQLELKSLISKSVSREKQEKGRANLAQLGALYQSSWDSQFVSGGEECFLISQFCCEVRKDEHAEKENTYTSYLDKFFSRKEDSEMLETEPVEEGKRGERGREAGLLSGGGEFLLSKQLESIGTPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHKTYSSILKHLN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
189	Phosphorylation	YSHKGHLSEGLVTKW CCCCCCCCCCCCCCC	24.76	26824392
194	Phosphorylation	HLSEGLVTKWYRSPR CCCCCCCCCCCCCCC	22.39	25777480
363	Phosphorylation	RYHDCQFSEHDWPIH HHCCCCCCCCCCCCC	14.24	22322096
386	Phosphorylation	QLDPRALSDVTDEEE ECCHHHHCCCCCHHH	29.11	26824392
389	Phosphorylation	PRALSDVTDEEEVQV HHHHCCCCCHHHCCC	42.56	25619855
441	Phosphorylation	KYCDLECSHTCNYKT CEECEECCCCCCCCC	16.68	-
483	Phosphorylation	LSNWKEQSKEKSDKR CCCHHHHHHHHCCHH	43.82	-
554	Phosphorylation	KLNDLNSSVSQLELK HHHHHCCCHHHHHHH	25.39	26370283
556	Phosphorylation	NDLNSSVSQLELKSL HHHCCCHHHHHHHHH	30.88	-
675	Phosphorylation	LLSKQLESIGTPQFH HHHHHHHHCCCCCCC	34.60	26643407
678	Phosphorylation	KQLESIGTPQFHSPV HHHHHCCCCCCCCCC	16.55	26643407
683	Phosphorylation	IGTPQFHSPVGSPLK CCCCCCCCCCCCCCH	23.67	26643407
687	Phosphorylation	QFHSPVGSPLKSIQA CCCCCCCCCCHHCEE	27.56	26643407
691	Phosphorylation	PVGSPLKSIQATLTP CCCCCCHHCEEEECH	27.84	22807455
697	Phosphorylation	KSIQATLTPSAMKSS HHCEEEECHHHHCCC	15.73	22807455
699	Phosphorylation	IQATLTPSAMKSSPQ CEEEECHHHHCCCCC	35.44	27681418
703	Phosphorylation	LTPSAMKSSPQIPHK ECHHHHCCCCCCCCC	33.88	23684622
704	Phosphorylation	TPSAMKSSPQIPHKT CHHHHCCCCCCCCCC	18.46	23684622

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
189	S	Phosphorylation	Kinase	PAK1	O88643	Uniprot
189	S	Phosphorylation	Kinase	PAK2	Q8CIN4	Uniprot
189	S	Phosphorylation	Kinase	PAK3	Q61036	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
1	M	ubiquitylation	Ubiquitination at Met-1 leads to degradation by the proteasome pathway.	-
189	S	Phosphorylation	Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation.	15577943

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MK06_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MAPK2_MOUSE	Mapkapk2	physical	15538386
MAPK5_MOUSE	Mapkapk5	physical	15538386
HSPB1_MOUSE	Hspb1	physical	15538386

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.