MERL_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MERL_MOUSE
• UniProt AC: P46662
• Protein Name: Merlin
• Gene Name: Nf2
• Organism: Mus musculus (Mouse).
• Sequence Length: 596
• Subcellular Localization: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cytoplasm, cytoskeleton. Nucleus. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with DCAF1 in the nucleus. The intramolecular association of the FERM do
• Protein Description: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium..
• Protein Sequence: MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPPPLPPDIPSFDIIADSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDVLHSESSDRGGPSSKHNTIKKLTLQSAKSRVAFFEEL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Phosphorylation	GAIASRMSFSSLKRK HHHHHCCCHHHHCCC	22.37	29514104
12	Phosphorylation	IASRMSFSSLKRKQP HHHCCCHHHHCCCCC	27.70	26643407
13	Phosphorylation	ASRMSFSSLKRKQPK HHCCCHHHHCCCCCC	35.58	29514104
66	Phosphorylation	TWFFGLQYTIKDTVA CEEEEEEEEEHHHHH	18.57	21183079
80	Ubiquitination	AWLKMDKKVLDHDVS HHHHCCHHHHCCCCC	43.71	-
315	Phosphorylation	MRRRKADSLEVQQMK HHHHHCHHHHHHHHH	31.15	-
364	Ubiquitination	ERRLLQMKEEATMAN HHHHHHHHHHHHHHH	39.17	22790023
391	Phosphorylation	LAEKAQITEEEAKLL HHHHHCCCHHHHHHH	25.84	21454597
396	Ubiquitination	QITEEEAKLLAQKAA CCCHHHHHHHHHHHH	47.52	22790023
439	Acetylation	EAEVLALKMAEESER HHHHHHHHHHHHHHH	30.38	7622427
518	Phosphorylation	DTDMKRLSMEIEKEK CCCCHHHHHHHHHHH	20.57	26824392
528	Phosphorylation	IEKEKVEYMEKSKHL HHHHHHHHHHHHHHH	17.35	29514104
532	Phosphorylation	KVEYMEKSKHLQEQL HHHHHHHHHHHHHHH	16.41	29514104
543	Acetylation	QEQLNELKTEIEALK HHHHHHHHHHHHHHH	37.62	8275655
568	Methylation	LHSESSDRGGPSSKH HHCCCCCCCCCCCCC	54.65	58858525
573	Phosphorylation	SDRGGPSSKHNTIKK CCCCCCCCCCHHHHH	40.86	25338131
582	Phosphorylation	HNTIKKLTLQSAKSR CHHHHHHHHHCHHHH	31.47	22817900
588	Phosphorylation	LTLQSAKSRVAFFEE HHHHCHHHHHHHHHC	31.53	29514104

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
518	S	Phosphorylation	Kinase	PAK	-	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Dcaf1	Q80TR8	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
518	S	Phosphorylation	Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail.	-
518	S	Phosphorylation	The dephosphorylation of Ser-518 favors the interaction with NOP53.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MERL_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CTNB1_MOUSE	Ctnnb1	physical	12695331
GRB2_MOUSE	Grb2	physical	16405865
DCAF1_HUMAN	VPRBP	physical	24706749
BD1L1_HUMAN	BOD1L1	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.