MCM4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MCM4_MOUSE
• UniProt AC: P49717
• Protein Name: DNA replication licensing factor MCM4
• Gene Name: Mcm4
• Organism: Mus musculus (Mouse).
• Sequence Length: 862
• Subcellular Localization: Nucleus.
• Protein Description: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity..
• Protein Sequence: MSSPASTPSRRSSRRGRVTPTQSLRSEESRSSPNRRRRGEDSSTGELLPMPTSPGADLQSPPAQNALFSSPPQMHSLAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSARKGLQVDLQSDGAAAEDIVPSEQSLGQKLVIWGTDVNVATCKENFQRFLQCFTDPLAKEEENVGIDITQPLYMQQLGEINITGEPFLNVNCEHIKSFSKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKSMRNLNPEDIDQLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEIDRGRIAEPCSCVHCHTTHSMALIHNRSFFSDKQMIKLQESPEDMPAGQTPHTIVLFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVKLLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVSLYYQSEEQVEEEFLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVNMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRFSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALRKLILSKGKTPALKYQQLFEDIRGQSDTAITKDMFEEALRALADDDFLTVTGKTVRLL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSSPASTPS ------CCCCCCCCC	45.06	-
2	Phosphorylation	------MSSPASTPS ------CCCCCCCCC	45.06	23684622
3	Phosphorylation	-----MSSPASTPSR -----CCCCCCCCCC	24.16	26824392
6	Phosphorylation	--MSSPASTPSRRSS --CCCCCCCCCCCCC	44.21	25263469
7	Phosphorylation	-MSSPASTPSRRSSR -CCCCCCCCCCCCCC	27.65	26824392
9	Phosphorylation	SSPASTPSRRSSRRG CCCCCCCCCCCCCCC	40.18	23684622
12	Phosphorylation	ASTPSRRSSRRGRVT CCCCCCCCCCCCCCC	27.38	29514104
13	Phosphorylation	STPSRRSSRRGRVTP CCCCCCCCCCCCCCC	24.96	29514104
19	Phosphorylation	SSRRGRVTPTQSLRS CCCCCCCCCCHHHCC	21.18	22942356
21	Phosphorylation	RRGRVTPTQSLRSEE CCCCCCCCHHHCCCC	22.79	28833060
23	Phosphorylation	GRVTPTQSLRSEESR CCCCCCHHHCCCCCC	28.38	27149854
26	Phosphorylation	TPTQSLRSEESRSSP CCCHHHCCCCCCCCC	51.51	27087446
29	Phosphorylation	QSLRSEESRSSPNRR HHHCCCCCCCCCCCC	33.15	24453211
31	Phosphorylation	LRSEESRSSPNRRRR HCCCCCCCCCCCCCC	60.47	29895711
32	Phosphorylation	RSEESRSSPNRRRRG CCCCCCCCCCCCCCC	25.69	27087446
43	Phosphorylation	RRRGEDSSTGELLPM CCCCCCCCCCCCCCC	53.40	23649490
52	Phosphorylation	GELLPMPTSPGADLQ CCCCCCCCCCCCCCC	41.10	22006019
53	Phosphorylation	ELLPMPTSPGADLQS CCCCCCCCCCCCCCC	18.57	20139300
60	Phosphorylation	SPGADLQSPPAQNAL CCCCCCCCCCHHHHH	39.90	23649490
76	Phosphorylation	SSPPQMHSLAIPLDF CCCCCCCCEEECCCC	17.72	23649490
87	Phosphorylation	PLDFDVSSPLTYGTP CCCCCCCCCCCCCCC	24.63	20139300
93	Phosphorylation	SSPLTYGTPSSRVEG CCCCCCCCCCHHCCC	15.02	21082442
95	Phosphorylation	PLTYGTPSSRVEGTP CCCCCCCCHHCCCCC	30.52	21082442
101	Phosphorylation	PSSRVEGTPRSGVRG CCHHCCCCCCCCCCC	11.30	24453211
104	Phosphorylation	RVEGTPRSGVRGTPV HCCCCCCCCCCCCCC	42.73	25263469
109	Phosphorylation	PRSGVRGTPVRQRPD CCCCCCCCCCCCCCC	14.19	26824392
119	Phosphorylation	RQRPDLGSARKGLQV CCCCCCCCCCCCEEE	32.00	26824392
130	Phosphorylation	GLQVDLQSDGAAAED CEEEEECCCCCCHHH	45.36	26824392
141	Phosphorylation	AAEDIVPSEQSLGQK CHHHCCCCCCHHCCC	37.06	25619855
144	Phosphorylation	DIVPSEQSLGQKLVI HCCCCCCHHCCCEEE	30.34	25619855
216	Phosphorylation	VNCEHIKSFSKNLYR CCHHHHHHHCHHHHH	33.86	24719451
219	Acetylation	EHIKSFSKNLYRQLI HHHHHHCHHHHHHHH	49.30	-
269	Phosphorylation	FNALKTKSMRNLNPE CCCCCCCCCCCCCHH	28.36	26745281
405	Phosphorylation	IRVNPRVSNVKSVYK EECCCCCCCCCCCEE	36.87	29895711
449	Acetylation	EKRVKLLKELSRKPD HHHHHHHHHHCCCCC	68.02	-
458	Phosphorylation	LSRKPDIYERLASAL HCCCCCHHHHHHHHH	11.46	-
463	Phosphorylation	DIYERLASALAPSIY CHHHHHHHHHCCHHH	28.54	18779572
468	Phosphorylation	LASALAPSIYEHEDI HHHHHCCHHHCCCCH	31.99	18779572
470	Phosphorylation	SALAPSIYEHEDIKK HHHCCHHHCCCCHHH	18.64	18779572
516	Phosphorylation	GDPGTSKSQLLQYVY CCCCCCHHHHHHHHH	26.31	30635358
521	Phosphorylation	SKSQLLQYVYNLVPR CHHHHHHHHHHHCCC	12.60	30635358
523	Phosphorylation	SQLLQYVYNLVPRGQ HHHHHHHHHHCCCCC	9.61	30635358
531	Phosphorylation	NLVPRGQYTSGKGSS HHCCCCCCCCCCCCC	13.00	30635358
532	Phosphorylation	LVPRGQYTSGKGSSA HCCCCCCCCCCCCCC	24.10	30635358
533	Phosphorylation	VPRGQYTSGKGSSAV CCCCCCCCCCCCCCE	33.31	30635358
535	Ubiquitination	RGQYTSGKGSSAVGL CCCCCCCCCCCCEEE	56.22	-
604	Glutathionylation	IAKAGIICQLNARTS HHHHCEEEECCCCCH	3.39	24333276
604	S-nitrosocysteine	IAKAGIICQLNARTS HHHHCEEEECCCCCH	3.39	-
604	S-nitrosylation	IAKAGIICQLNARTS HHHHCEEEECCCCCH	3.39	20925432
818	Acetylation	KGKTPALKYQQLFED CCCCHHHHHHHHHHH	43.02	22826441
818	Ubiquitination	KGKTPALKYQQLFED CCCCHHHHHHHHHHH	43.02	-
857	Acetylation	DFLTVTGKTVRLL-- CCEEECCCEEEEC--	34.19	23806337

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
3	S	Phosphorylation	Kinase	CDK2	P24941	PSP
3	S	Phosphorylation	Kinase	CDK2	P97377	PhosphoELM
7	T	Phosphorylation	Kinase	CDK1	P11440	PSP
19	T	Phosphorylation	Kinase	CDK1	P11440	PSP
19	T	Phosphorylation	Kinase	CDK2	P24941	PSP
19	T	Phosphorylation	Kinase	CDK2	P97377	PhosphoELM
32	S	Phosphorylation	Kinase	CDK1	P11440	PSP
53	S	Phosphorylation	Kinase	CDK2	P24941	PSP
53	S	Phosphorylation	Kinase	CDK2	P97377	PhosphoELM
87	S	Phosphorylation	Kinase	CDK1	P11440	PSP
109	T	Phosphorylation	Kinase	CDK1	P11440	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MCM4_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MCM4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FANCC_MOUSE	Fancc	genetic	24589582

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.