M3K12_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: M3K12_HUMAN
• UniProt AC: Q12852
• Protein Name: Mitogen-activated protein kinase kinase kinase 12
• Gene Name: MAP3K12
• Organism: Homo sapiens (Human).
• Sequence Length: 859
• Subcellular Localization: Cytoplasm. Membrane; Peripheral membrane protein.
• Protein Description: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro..
• Protein Sequence: MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTHVLQLHEQDAGGPGGAAGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKPHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCPKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRTAVPPHEPGGPGSPGGLGGGPSAWEACPPALRGLHHDLLLRKMSSSSPDLLSAALGSRGRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGEGVGLLGTGREGTSGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELTSSQRWPQSLNMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPPSEVIPGPEPSSLPIPHQELLRERGPPNSEDSDCDSTELDNSNSVDALRPPASLPP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MACLHETRTPSPSF -CCCCCCCCCCCCCC	29.15	22199227
9	Phosphorylation	ACLHETRTPSPSFGG CCCCCCCCCCCCCCH	35.22	25850435
11	Phosphorylation	LHETRTPSPSFGGFV CCCCCCCCCCCCHHH	32.34	22199227
13	Phosphorylation	ETRTPSPSFGGFVST CCCCCCCCCCHHHHH	40.64	25850435
19	Phosphorylation	PSFGGFVSTLSEASM CCCCHHHHHHCHHHH	22.75	27732954
20	Phosphorylation	SFGGFVSTLSEASMR CCCHHHHHHCHHHHH	29.06	27732954
37	Phosphorylation	DPDTSDCTPEKDLTP CCCCCCCCCCCCCCC	39.20	-
43 (in isoform 2)	Phosphorylation	-	35.95	22199227
43	Phosphorylation	CTPEKDLTPTHVLQL CCCCCCCCCCEEEEE	35.95	-
45 (in isoform 2)	Phosphorylation	-	21.27	27732954
163	Ubiquitination	DLKETDIKHLRKLKH CCCCCCHHHHHHCCC	39.12	-
253	Phosphorylation	YDDVVKISDFGTSKE HHHEEEHHHCCCCHH	22.59	24905233
257	Phosphorylation	VKISDFGTSKELSDK EEHHHCCCCHHHCCC	36.30	24905233
258	Phosphorylation	KISDFGTSKELSDKS EHHHCCCCHHHCCCC	25.22	24905233
262	Phosphorylation	FGTSKELSDKSTKMS CCCCHHHCCCCCCCC	44.05	24905233
265	Phosphorylation	SKELSDKSTKMSFAG CHHHCCCCCCCCHHH	37.95	24905233
266	Phosphorylation	KELSDKSTKMSFAGT HHHCCCCCCCCHHHH	36.18	24905233
269	Phosphorylation	SDKSTKMSFAGTVAW CCCCCCCCHHHHHHH	17.32	28348404
477	Phosphorylation	GLLKPHPSRGLLHGN CCCCCCCCCCCCCCC	35.63	-
500	Phosphorylation	RNVPQKLSPHSKRPD CCCCCCCCCCCCCCC	27.83	23312004
503	Phosphorylation	PQKLSPHSKRPDILK CCCCCCCCCCCCHHH	33.47	30576142
533	Phosphorylation	GLPGCPKGPPSPGRS CCCCCCCCCCCCCCC	25.21	27251275
536	Phosphorylation	GCPKGPPSPGRSRRG CCCCCCCCCCCCCCC	43.02	22617229
551	Phosphorylation	KTRHRKASAKGSCGD CCCCHHHCCCCCCCC	33.28	28555341
555	Phosphorylation	RKASAKGSCGDLPGL HHHCCCCCCCCCCCC	17.77	28985074
588	Phosphorylation	GGGPSAWEACPPALR CCCCCHHHHCCHHHH	39.86	27251275
607	Phosphorylation	DLLLRKMSSSSPDLL HHHHHHHHCCCHHHH	30.06	25850435
608	Phosphorylation	LLLRKMSSSSPDLLS HHHHHHHCCCHHHHH	31.19	22199227
609	Phosphorylation	LLRKMSSSSPDLLSA HHHHHHCCCHHHHHH	38.29	30576142
610	Phosphorylation	LRKMSSSSPDLLSAA HHHHHCCCHHHHHHH	25.12	22199227
615	Phosphorylation	SSSPDLLSAALGSRG CCCHHHHHHHHCCCC	20.84	22199227
620	Phosphorylation	LLSAALGSRGRGATG HHHHHHCCCCCCCCC	32.20	22199227
634	Phosphorylation	GGAGDPGSPPPARGD CCCCCCCCCCCCCCC	39.35	29255136
640	Phosphorylation	GSPPPARGDTPPSEG CCCCCCCCCCCCCCC	45.29	27251275
642	Phosphorylation	PPPARGDTPPSEGSA CCCCCCCCCCCCCCC	39.67	-
645	Phosphorylation	ARGDTPPSEGSAPGS CCCCCCCCCCCCCCC	56.65	-
652	Phosphorylation	SEGSAPGSTSPDSPG CCCCCCCCCCCCCCC	25.34	27732954
653	Phosphorylation	EGSAPGSTSPDSPGG CCCCCCCCCCCCCCC	50.63	27732954
654	Phosphorylation	GSAPGSTSPDSPGGA CCCCCCCCCCCCCCC	28.54	27732954
657	Phosphorylation	PGSTSPDSPGGAKGE CCCCCCCCCCCCCCC	28.91	27732954
695	Phosphorylation	RGGSRAGSQHLTPAA CCCCCCCCCCCCHHH	17.28	21712546
699	Phosphorylation	RAGSQHLTPAALLYR CCCCCCCCHHHHHHH	14.57	21712546
728	Phosphorylation	EEEGEVDSEVELTSS CCCCCCCCCEEECCC	49.03	27251275
757	Phosphorylation	TFSSENPSDGEEGTA HCCCCCCCCCCCCCC	70.59	22817900
788	Phosphorylation	DERPDERSDDMCSQG CCCCCCCCCCCHHCC	35.60	22210691
793	Phosphorylation	ERSDDMCSQGSEIPL CCCCCCHHCCCCCCC	30.82	22210691
796	Phosphorylation	DDMCSQGSEIPLDPP CCCHHCCCCCCCCCC	25.39	22210691

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	STUB1	Q9UNE7	PMID:16931512

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of M3K12_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of M3K12_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
JIP1_HUMAN	MAPK8IP1	physical	10490659
JIP2_HUMAN	MAPK8IP2	physical	10490659
MP2K7_HUMAN	MAP2K7	physical	10187804

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-634 AND SER-757, ANDMASS SPECTROMETRY.
PubMed: 19369195