M2K5_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: M2K5_ARATH
• UniProt AC: Q8RXG3
• Protein Name: Mitogen-activated protein kinase kinase 5
• Gene Name: MKK5
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 348
• Protein Description: Involved in the second phase of hydrogen peroxide generation during hypersensitive response-like cell death. Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. Activates by phosphorylation the downstream MPK3 and MPK6. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs. MKK4 and MKK5 participate in the regulation of floral organ abscission. Target of the Pseudomonas syringae type III effector HopF2, that inhibits the activation of the downstream MPK6 and PAMP-triggered immunity. Plays a critical role in high light stress tolerance by the mediation of the Cu/Zn SODs CSD1 and CSD2 gene expression. Phosphorylates BZR1 in vitro..
• Protein Sequence: MKPIQSPSGVASPMKNRLRKRPDLSLPLPHRDVALAVPLPLPPPSSSSSAPASSSAISTNISAAKSLSELERVNRIGSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEILRSVDHPNVVKCHDMFDHNGEIQVLLEFMDQGSLEGAHIWQEQELADLSRQILSGLAYLHRRHIVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNHGRYDGYAGDVWSLGVSILEFYLGRFPFAVSRQGDWASLMCAICMSQPPEAPATASQEFRHFVSCCLQSDPPKRWSAQQLLQHPFILKATGGPNLRQMLPPPRPLPSAS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MKPIQSPSGVASP --CCCCCCCCCCCCC	23.04	-
12	Phosphorylation	QSPSGVASPMKNRLR CCCCCCCCCCCCCHH	24.11	23111157
215	Phosphorylation	VSRILAQTMDPCNSS HHHHHHHHCCCCCCC	20.24	11875555
221	Phosphorylation	QTMDPCNSSVGTIAY HHCCCCCCCCCCCEE	32.87	23341468
225	Phosphorylation	PCNSSVGTIAYMSPE CCCCCCCCCEECCHH	10.57	27679653
313	ADP-ribosylation	LQSDPPKRWSAQQLL HCCCCCCCCCHHHHH	38.05	-
313	ADP-ribosylation	LQSDPPKRWSAQQLL HCCCCCCCCCHHHHH	38.05	20571112
315	Phosphorylation	SDPPKRWSAQQLLQH CCCCCCCCHHHHHHC	21.89	25368622
329	Phosphorylation	HPFILKATGGPNLRQ CCEEEECCCCCCHHH	40.87	25368622

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
6	S	Phosphorylation	Kinase	ASK7	Q39011	Uniprot
221	S	Phosphorylation	Kinase	ASK7	Q39011	Uniprot
225	T	Phosphorylation	Kinase	ASK7	Q39011	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
215	T	Phosphorylation	Phosphorylation at Thr-215 and Ser-221 by MAP kinase kinase kinases positively regulates kinase activity.	11687590
221	S	Phosphorylation	Phosphorylation at Thr-215 and Ser-221 by MAP kinase kinase kinases positively regulates kinase activity.	11687590
313	R	ADP-ribosylation	ADP-ribosylation at Arg-313 by P.syringae type III effector HopF2 reduces the ability of the protein to phosphorylate downstream MPK6.	20571112

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of M2K5_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BZR1_ARATH	BZR1	physical	24019147
M3K1_ARATH	MEKK1	physical	26136265

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"MAP kinase signalling cascade in Arabidopsis innate immunity.";
Asai T., Tena G., Plotnikova J., Willmann M.R., Chiu W.-L.,Gomez-Gomez L., Boller T., Ausubel F.M., Sheen J.;
Nature 415:977-983(2002).
Cited for: FUNCTION, ENZYME REGULATION, PHOSPHORYLATION AT THR-215 AND SER-221,AND MUTAGENESIS OF LYS-99; THR-215 AND SER-221.
PubMed: 11875555