KS6A5_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KS6A5_MOUSE
• UniProt AC: Q8C050
• Protein Name: Ribosomal protein S6 kinase alpha-5
• Gene Name: Rps6ka5
• Organism: Mus musculus (Mouse).
• Sequence Length: 863
• Subcellular Localization: Nucleus.
• Protein Description: Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (By similarity)(PubMed:11553624, PubMed:11909979, PubMed:16806820). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. [PubMed: 11909979 Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression]
• Protein Sequence: MEGEGGGSGGAGTSGDSGDGGEQLLTVKHELRTANLTGHAEKVGIENFELLKVLGTGAYGKVFLVRKISGHDAGKLYAMKVLKKATIVQKAKTTEHTRTERQVLEHIRQSPFLVTLHYAFQTETKLHLILDYINGGELFTHLSQRERFTEHEVQIYVGEIVLALEHLHKLGIIYRDIKLENILLDSNGHVVLTDFGLSKEFVADETERAYSFCGTIEYMAPDIVRGGDSGHDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQAEISRRILKSEPPYPQEMSTVAKDLLQRLLMKDPKKRLGCGPRDAEEIKEHLFFEKIKWDDLAAKKVPAPFKPVIRDELDVSNFAEEFTEMDPTYSPAALPQSSERLFQGYSFVAPSILFKRNAAVIDPLQFHMGVDRPGVTNVARSAMMKDSPFYQHYDLDLKDKPLGEGSFSICRKCVHKKTNQAFAVKIISKRMEANTQKEITALKLCEGHPNIVKLHEVFHDQVAASAQPPGQVVLCSLLLLALLFNRSLTRKPVTWTWLVHSTSQLPPLPPPMPEIVLFILLSDNGQLHTFLVMELLNGGELFERIKRKKHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLLFTDENDNLEIKVIDFGFARLKPPDNQPLKTPCFTLHYAAPELLTHNGYDESCDLWSLGVILYTMLSGQVPFQSHDRSLTCTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKDLIQGLLTVDPNKRLKMSGLRYNEWLQDGSQLSSNPLMTPDILGSSGAAVHTCVKATFHAFNKYKREGFCLQNVDKAPLAKRRKMKRTSTSTETRSSSSESSRSSSSQSHGKTTPTKTLQPSNPTEGSNPDTLFQFSD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8	Phosphorylation	MEGEGGGSGGAGTSG CCCCCCCCCCCCCCC	37.01	29514104
14	Phosphorylation	GSGGAGTSGDSGDGG CCCCCCCCCCCCCCC	39.42	25338131
17	Phosphorylation	GAGTSGDSGDGGEQL CCCCCCCCCCCCCEE	42.16	25338131
37	Phosphorylation	ELRTANLTGHAEKVG HHHHCCCCCCHHHHC	27.08	-
56	Phosphorylation	ELLKVLGTGAYGKVF EEEEHHCCCCCCEEE	17.93	26239621
59	Phosphorylation	KVLGTGAYGKVFLVR EHHCCCCCCEEEEEE	20.87	26239621
198	Phosphorylation	VLTDFGLSKEFVADE EEECCCCCHHHHCCC	30.44	23737553
211	Phosphorylation	DETERAYSFCGTIEY CCHHHHHHHHCCEEE	17.05	22817900
215	Phosphorylation	RAYSFCGTIEYMAPD HHHHHHCCEEEECCC	16.30	21183079
345	Phosphorylation	IRDELDVSNFAEEFT CCCCCCHHHHHHHHH	26.37	29899451
352	Phosphorylation	SNFAEEFTEMDPTYS HHHHHHHHHCCCCCC	33.41	22802335
358	Phosphorylation	FTEMDPTYSPAALPQ HHHCCCCCCCCCCCC	20.32	22802335
359	Phosphorylation	TEMDPTYSPAALPQS HHCCCCCCCCCCCCC	15.56	11994045
366	Phosphorylation	SPAALPQSSERLFQG CCCCCCCCCCHHHCC	32.02	25338131
374	Phosphorylation	SERLFQGYSFVAPSI CCHHHCCCCEECHHH	6.53	22322096
375	Phosphorylation	ERLFQGYSFVAPSIL CHHHCCCCEECHHHH	21.77	22322096
380	Phosphorylation	GYSFVAPSILFKRNA CCCEECHHHHHCCCC	23.37	22322096
645	Phosphorylation	PDNQPLKTPCFTLHY CCCCCCCCCCEEEEE	32.73	11994045
694	Phosphorylation	QSHDRSLTCTSAVEI CCCCCCCEECCHHHH	18.25	17117922
711	Phosphorylation	KIKKGDFSFEGEAWK HHHCCCCCCCCHHHH	27.42	17117922
721	Phosphorylation	GEAWKNVSQEAKDLI CHHHHCCCHHHHHHH	31.53	17117922
755	Phosphorylation	NEWLQDGSQLSSNPL HHHHCCCCCCCCCCC	35.92	22817900
758	Phosphorylation	LQDGSQLSSNPLMTP HCCCCCCCCCCCCCH	22.04	22817900
759	Phosphorylation	QDGSQLSSNPLMTPD CCCCCCCCCCCCCHH	51.57	19060867
764	Phosphorylation	LSSNPLMTPDILGSS CCCCCCCCHHHHCCC	26.03	22817900
770	Phosphorylation	MTPDILGSSGAAVHT CCHHHHCCCCCHHHH	23.35	21183079
813	Phosphorylation	KRRKMKRTSTSTETR HHHCCCCCCCCCCCC	30.73	29899451
814	Phosphorylation	RRKMKRTSTSTETRS HHCCCCCCCCCCCCC	25.13	23684622
815	Phosphorylation	RKMKRTSTSTETRSS HCCCCCCCCCCCCCC	38.77	23684622
816	Phosphorylation	KMKRTSTSTETRSSS CCCCCCCCCCCCCCC	24.82	23684622
819	Phosphorylation	RTSTSTETRSSSSES CCCCCCCCCCCCCCC	35.62	29899451
821	Phosphorylation	STSTETRSSSSESSR CCCCCCCCCCCCCCC	41.83	29899451
822	Phosphorylation	TSTETRSSSSESSRS CCCCCCCCCCCCCCC	34.29	29899451
823	Phosphorylation	STETRSSSSESSRSS CCCCCCCCCCCCCCC	39.10	23684622
824	Phosphorylation	TETRSSSSESSRSSS CCCCCCCCCCCCCCC	43.37	23684622
829	Phosphorylation	SSSESSRSSSSQSHG CCCCCCCCCCCCCCC	36.20	26160508
830	Phosphorylation	SSESSRSSSSQSHGK CCCCCCCCCCCCCCC	32.66	26160508
831	Phosphorylation	SESSRSSSSQSHGKT CCCCCCCCCCCCCCC	33.37	26160508
832	Phosphorylation	ESSRSSSSQSHGKTT CCCCCCCCCCCCCCC	37.34	26160508
834	Phosphorylation	SRSSSSQSHGKTTPT CCCCCCCCCCCCCCC	35.83	26160508
838	Phosphorylation	SSQSHGKTTPTKTLQ CCCCCCCCCCCCCCC	42.47	26160508
839	Phosphorylation	SQSHGKTTPTKTLQP CCCCCCCCCCCCCCC	32.23	25266776
841	Phosphorylation	SHGKTTPTKTLQPSN CCCCCCCCCCCCCCC	34.27	26160508
843	Phosphorylation	GKTTPTKTLQPSNPT CCCCCCCCCCCCCCC	32.86	27087446
847	Phosphorylation	PTKTLQPSNPTEGSN CCCCCCCCCCCCCCC	42.34	26160508
853	Phosphorylation	PSNPTEGSNPDTLFQ CCCCCCCCCCCCCCC	38.67	28833060
857	Phosphorylation	TEGSNPDTLFQFSD- CCCCCCCCCCCCCC-	30.63	23984901
862	Phosphorylation	PDTLFQFSD------ CCCCCCCCC------	31.46	24925903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
359	S	Phosphorylation	Kinase	MAPK1	P63085	Uniprot
359	S	Phosphorylation	Kinase	MAPK3	Q63844	Uniprot
359	S	Phosphorylation	Kinase	MAPK14	P47811	Uniprot
645	T	Phosphorylation	Kinase	MAPK1	P28482	GPS
645	T	Phosphorylation	Kinase	MAPK1	P63085	Uniprot
645	T	Phosphorylation	Kinase	MAPK3	Q63844	Uniprot
645	T	Phosphorylation	Kinase	MAPK14	Q16539	GPS
645	T	Phosphorylation	Kinase	MAPK14	P47811	Uniprot
764	T	Phosphorylation	Kinase	MAPK1	P28482	GPS
764	T	Phosphorylation	Kinase	MAPK14	Q16539	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
211	S	Phosphorylation	Ser-375 and Ser-211 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.	-
359	S	Phosphorylation	Phosphorylated at Ser-359, Thr-645 and Thr-764 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha.	-
375	S	Phosphorylation	Ser-375 and Ser-211 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.	-
375	S	Phosphorylation	Ser-375 and Thr-645 phosphorylation is required for kinase activity.	-
645	T	Phosphorylation	Phosphorylated at Ser-359, Thr-645 and Thr-764 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha.	-
645	T	Phosphorylation	Ser-375 and Thr-645 phosphorylation is required for kinase activity.	-
764	T	Phosphorylation	Phosphorylated at Ser-359, Thr-645 and Thr-764 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha.	21183079
814	S	Phosphorylation	Autophosphorylated at Ser-814, Ser-816 and Ser-822 by the N-terminal kinase domain (By similarity).	-
816	S	Phosphorylation	Autophosphorylated at Ser-814, Ser-816 and Ser-822 by the N-terminal kinase domain (By similarity).	-
822	S	Phosphorylation	Autophosphorylated at Ser-814, Ser-816 and Ser-822 by the N-terminal kinase domain (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KS6A5_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SMCA4_MOUSE	Smarca4	physical	20129940
1433Z_MOUSE	Ywhaz	physical	20129940
ATF2_MOUSE	Atf2	physical	15192015

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.