KISS1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KISS1_HUMAN
• UniProt AC: Q15726
• Protein Name: Metastasis-suppressor KiSS-1
• Gene Name: KISS1
• Organism: Homo sapiens (Human).
• Sequence Length: 138
• Subcellular Localization: Secreted.
• Protein Description: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood..
• Protein Sequence: MNSLVSWQLLLFLCATHFGEPLEKVASVGNSRPTGQQLESLGLLAPGEQSLPCTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRSAGRG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
112	Phosphorylation	REKDLPNYNWNSFGL CCCCCCCCCCCCHHH	21.44	14729942
121	Phenylalanine amide	WNSFGLRFGKREAAP CCCHHHHCCCCCCCC	19.04	-
121	Amidation	WNSFGLRFGKREAAP CCCHHHHCCCCCCCC	19.04	11385580
134	Phosphorylation	APGNHGRSAGRG--- CCCCCCCCCCCC---	39.40	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of KISS1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of KISS1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KISS1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
A4_HUMAN	APP	physical	21832049

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 614842: Hypogonadotropic hypogonadism 13 with or without anosmia (HH13)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Amidation

"Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor.";
Ohtaki T., Shintani Y., Honda S., Matsumoto H., Hori A., Kanehashi K.,Terao Y., Kumano S., Takatsu Y., Masuda Y., Ishibashi Y., Watanabe T.,Asada M., Yamada T., Suenaga M., Kitada C., Usuki S., Kurokawa T.,Onda H., Nishimura O., Fujino M.;
Nature 411:613-617(2001).
Cited for: PROTEIN SEQUENCE OF 68-88, AMIDATION AT PHE-121, FUNCTION, AND TISSUESPECIFICITY.
PubMed: 11385580

Phosphorylation

"Robust phosphoproteomic profiling of tyrosine phosphorylation sitesfrom human T cells using immobilized metal affinity chromatography andtandem mass spectrometry.";
Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M.,Peters E.C.;
Anal. Chem. 76:2763-2772(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-112, AND MASSSPECTROMETRY.
PubMed: 15144186