IF_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: IF_HUMAN
• UniProt AC: P27352
• Protein Name: Gastric intrinsic factor
• Gene Name: GIF
• Organism: Homo sapiens (Human).
• Sequence Length: 417
• Subcellular Localization: Secreted.
• Protein Description: Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis..
• Protein Sequence: MAWFALYLLSLLWATAGTSTQTQSSCSVPSAQEPLVNGIQVLMENSVTSSAYPNPSILIAMNLAGAYNLKAQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKVSILQRQMENWAPSSPNAEASAFYGPSLAILALCQKNSEATLPIAVRFAKTLLANSSPFNVDTGAMATLALTCMYNKIPVGSEEGYRSLFGQVLKDIVEKISMKIKDNGIIGDIYSTGLAMQALSVTPEPSKKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTYLDVPQVTCSPDHEVQPTLPSNPGPGPTSASNITVIYTINNQLRGVELLFNETINVSVKSGSVLLVVLEEAQRKNPMFKFETTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
160	Phosphorylation	IAVRFAKTLLANSSP HHHHHHHHHHHCCCC	24.47	29083192
165	Phosphorylation	AKTLLANSSPFNVDT HHHHHHCCCCCCCCH	33.51	29083192
166	Phosphorylation	KTLLANSSPFNVDTG HHHHHCCCCCCCCHH	33.06	29083192
172	Phosphorylation	SSPFNVDTGAMATLA CCCCCCCHHHHHHHH	23.66	29083192
191	Phosphorylation	YNKIPVGSEEGYRSL HCCCCCCCHHHHHHH	32.48	-
274	Phosphorylation	SIAQILPSLKGKTYL CHHHHHHHCCCCEEE	38.99	24719451
297	Phosphorylation	PDHEVQPTLPSNPGP CCCCCCCCCCCCCCC	33.92	-
311	N-linked_Glycosylation	PGPTSASNITVIYTI CCCCCCCCEEEEEEE	33.40	20237569
317	Phosphorylation	SNITVIYTINNQLRG CCEEEEEEECCEEEC	13.20	-
330	N-linked_Glycosylation	RGVELLFNETINVSV ECEEEEEECCEEEEE	45.53	UniProtKB CARBOHYD
334	N-linked_Glycosylation	LLFNETINVSVKSGS EEEECCEEEEECCCC	27.68	UniProtKB CARBOHYD
413	N-linked_Glycosylation	NHEHITANFTQY--- CCCCCCCCCCCC---	31.67	20237569

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of IF_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of IF_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of IF_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FBX2_HUMAN	FBXO2	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 261000: Hereditary intrinsic factor deficiency (IFD)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

N-linked Glycosylation

"Structural basis for receptor recognition of vitamin-B(12)-intrinsicfactor complexes.";
Andersen C.B., Madsen M., Storm T., Moestrup S.K., Andersen G.R.;
Nature 464:445-448(2010).
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF 25-417 IN COMPLEX WITHCOBALAMIN AND CUBN, INTERACTION WITH CUBN, DISULFIDE BONDS, ANDGLYCOSYLATION AT ASN-311 AND ASN-413.
PubMed: 20237569

"Crystal structure of human intrinsic factor: cobalamin complex at2.6-A resolution.";
Mathews F.S., Gordon M.M., Chen Z., Rajashankar K.R., Ealick S.E.,Alpers D.H., Sukumar N.;
Proc. Natl. Acad. Sci. U.S.A. 104:17311-17316(2007).
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 19-417 IN COMPLEX WITHCOBALAMIN, DISULFIDE BONDS, AND GLYCOSYLATION AT ASN-413.
PubMed: 17954916