dbPTM

HIPK1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	HIPK1_MOUSE
UniProt AC	O88904
Protein Name	Homeodomain-interacting protein kinase 1
Gene Name	Hipk1
Organism	Mus musculus (Mouse).
Sequence Length	1210
Subcellular Localization	Nucleus. Cytoplasm. Predominantly nuclear. Translocates from nucleus to cytoplasm in response to stress stimuli via SENP1-mediated desumoylation.
Protein Description	Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation..
Protein Sequence	MASQLQVFSPPSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQASSSHQVANFNLPAYDQGLLLPAPAVEHIVVTAADSSGSAATATFQSSQTLTHRSNVSLLEPYQKCGLKRKSEEVESNGSVQIIEEHPPLMLQNRTVVGAAATTTTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKNHPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRREYIDLLKKMLTIDADKRITPLKTLNHQFVTMSHLLDFPHSSHVKSCFQNMEICKRRVHMYDTVSQIKSPFTTHVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLANSDVSLLNYQSALYPSSAAPVPGVAQQGVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPVRMDNAVPIVPQAPAAQPLQIQSGVLTQGSCTPLMVATLHPQVATITPQYAVPFTLSCAAGRPALVEQTAAVLQAWPGGTQQILLPSAWQQLPGVALHNSVQPAAVIPEAMGSSQQLADWRNAHSHGNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSSLPSKKNKQSAPVSSKSSLEVLPSQVYSLVGSSPLRTTSSYNSLVPVQDQHQPIIIPDTPSPPVSVITIRSDTDEEEDNKYKPNSSSLKARSNVISYVTVNDSPDSDSSLSSPHPTDTLSALRGNSGTLLEGPGRPAADGIGTRTIIVPPLKTQLGDCTVATQASGLLSSKTKPVASVSGQSSGCCITPTGYRAQRGGASAVQPLNLSQNQQSSSASTSQERSSNPAPRRQQAFVAPLSQAPYAFQHGSPLHSTGHPHLAPAPAHLPSQPHLYTYAAPTSAAALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSLLTSASVAPAQYQHQFATQSYIGSSRGSTIYTGYPLSPTKISQYSYL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	ASQLQVFSPPSVSSS CCCCEECCCCCCCHH	36.72	25293948
12	Phosphorylation	LQVFSPPSVSSSAFC CEECCCCCCCHHHHC	37.71	25293948
14	Phosphorylation	VFSPPSVSSSAFCSA ECCCCCCCHHHHCCC	23.94	25293948
15	Phosphorylation	FSPPSVSSSAFCSAK CCCCCCCHHHHCCCC	24.43	25293948
16	Phosphorylation	SPPSVSSSAFCSAKK CCCCCCHHHHCCCCC	20.18	25293948
151	O-linked_Glycosylation	PLMLQNRTVVGAAAT CCEECCCEEEEEEEE	27.39	55412217
190	Phosphorylation	LCSMTNSYEVLEFLG HHHHCCHHHHHHHHC	16.48	-
264	Phosphorylation	CFQHKNHTCLVFEML HHHCCCCEEEHHHHH	19.54	23485397
350	Phosphorylation	HVSKAVCSTYLQSRY HHHHHHHHHHHHHCC	17.31	30635358
351	Phosphorylation	VSKAVCSTYLQSRYY HHHHHHHHHHHHCCC	24.11	25521595
352	Phosphorylation	SKAVCSTYLQSRYYR HHHHHHHHHHHCCCC	6.10	23485397
355	Phosphorylation	VCSTYLQSRYYRAPE HHHHHHHHCCCCCCH	21.47	21082442
421	Ubiquitination	YLLSAGTKTTRFFNR HHHCCCCCCEECCCC	47.16	-
459	Phosphorylation	KSKEARKYIFNCLDD CCHHHHHHHHHHHHH	12.75	26026062
479	Phosphorylation	MSTDLEGTDMLAEKA CCCCCCCHHHHHHHC	14.62	26026062
840	Phosphorylation	VRQQQSSSLPSKKNK HHHHHCCCCCCCCCC	49.93	29514104
849	Phosphorylation	PSKKNKQSAPVSSKS CCCCCCCCCCCCCHH	35.83	25777480
854	Phosphorylation	KQSAPVSSKSSLEVL CCCCCCCCHHHHHCC	36.47	25777480
863	Phosphorylation	SSLEVLPSQVYSLVG HHHHCCHHHHHHHHC	28.28	25777480
866	Phosphorylation	EVLPSQVYSLVGSSP HCCHHHHHHHHCCCC	6.66	25777480
867	Phosphorylation	VLPSQVYSLVGSSPL CCHHHHHHHHCCCCC	20.09	25777480
871	Phosphorylation	QVYSLVGSSPLRTTS HHHHHHCCCCCCCCC	22.69	26643407
872	Phosphorylation	VYSLVGSSPLRTTSS HHHHHCCCCCCCCCC	23.25	26643407
938	Phosphorylation	SNVISYVTVNDSPDS CCEEEEEEECCCCCC	12.83	29895711
945	Phosphorylation	TVNDSPDSDSSLSSP EECCCCCCCCCCCCC	42.66	29895711
947	Phosphorylation	NDSPDSDSSLSSPHP CCCCCCCCCCCCCCC	36.81	29895711
948	Phosphorylation	DSPDSDSSLSSPHPT CCCCCCCCCCCCCCC	36.94	29895711
950	Phosphorylation	PDSDSSLSSPHPTDT CCCCCCCCCCCCCCH	43.71	29895711
957	Phosphorylation	SSPHPTDTLSALRGN CCCCCCCHHHHHCCC	25.70	29895711
959	Phosphorylation	PHPTDTLSALRGNSG CCCCCHHHHHCCCCC	27.61	22006019
965	Phosphorylation	LSALRGNSGTLLEGP HHHHCCCCCCCCCCC	35.66	28066266
967	Phosphorylation	ALRGNSGTLLEGPGR HHCCCCCCCCCCCCC	28.17	28066266
1027	Phosphorylation	QSSGCCITPTGYRAQ CCCCEEECCCCCCCC	10.82	26643407
1029	Phosphorylation	SGCCITPTGYRAQRG CCEEECCCCCCCCCC	37.89	26643407
1191	Phosphorylation	YIGSSRGSTIYTGYP HCCCCCCCEEECCCC	15.55	25777480
1192	Phosphorylation	IGSSRGSTIYTGYPL CCCCCCCEEECCCCC	22.34	25777480
1194	Phosphorylation	SSRGSTIYTGYPLSP CCCCCEEECCCCCCC	8.32	25777480
1195	Phosphorylation	SRGSTIYTGYPLSPT CCCCEEECCCCCCCC	26.79	25777480
1197	Phosphorylation	GSTIYTGYPLSPTKI CCEEECCCCCCCCCC	8.00	25777480
1200	Phosphorylation	IYTGYPLSPTKISQY EECCCCCCCCCCCCC	26.47	27566939
1202	Phosphorylation	TGYPLSPTKISQYSY CCCCCCCCCCCCCCC	37.32	29895711

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of HIPK1_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of HIPK1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of HIPK1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
MDM2_MOUSE	Mdm2	physical	22173032

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of HIPK1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-352, AND MASSSPECTROMETRY.	17114649 [Abstract]
"Immunoaffinity profiling of tyrosine phosphorylation in cancercells."; Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,Zha X.-M., Polakiewicz R.D., Comb M.J.; Nat. Biotechnol. 23:94-101(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-352, AND MASSSPECTROMETRY.	15592455 [Abstract]