HCN4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HCN4_HUMAN
• UniProt AC: Q9Y3Q4
• Protein Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• Gene Name: HCN4
• Organism: Homo sapiens (Human).
• Sequence Length: 1203
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein .
• Protein Description: Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat. May contribute to the native pacemaker currents in neurons (Ih). May mediate responses to sour stimuli..
• Protein Sequence: MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPSRRSIRLRPLPSPSPSAAAGGTESRSSALGAADSEGPARGAGKSSTNGDCRRFRGSLASLGSRGGGSGGTGSGSSHGHLHDSAEERRLIAEGDASPGEDRTPPGLAAEPERPGASAQPAASPPPPQQPPQPASASCEQPSVDTAIKVEGGAAAGDQILPEAEVRLGQAGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFMVDFISSIPVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPDDCWVSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMSDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQGKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVQHDREMAHCAHRVQAAASATPTPTPVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGSGLGNLGAGQTPRHLKRLQSLIPSALGSASPASSPSQVDTPSSSSFHIQQLAGFSAPAGLSPLLPSSSSSPPPGACGSPSAPTPSAGVAATTIAGFGHFHKALGGSLSSSDSPLLTPLQPGARSPQAAQPSPAPPGARGGLGLPEHFLPPPPSSRSPSSSPGQLGQPPGELSLGLATGPLSTPETPPRQPEPPSLVAGASGGASPVGFTPRGGLSPPGHSPGPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESMAAFPLFPRAGGGSGGSGSSGGLGPPGRPYGAIPGQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAGPQREPGARPEPVRSKLPSNL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
13	Phosphorylation	PSMRKRLYSLPQQVG HHHHHHHHCCCHHHC	16.52	23312004
14	Phosphorylation	SMRKRLYSLPQQVGA HHHHHHHCCCHHHCC	37.86	32142685
55	Phosphorylation	IRLRPLPSPSPSAAA EEEEECCCCCCCCCC	45.77	32142685
57	Phosphorylation	LRPLPSPSPSAAAGG EEECCCCCCCCCCCC	35.51	32142685
67	Phosphorylation	AAAGGTESRSSALGA CCCCCCCCCHHHCCC	36.98	-
69	Phosphorylation	AGGTESRSSALGAAD CCCCCCCHHHCCCCC	29.28	-
70	Phosphorylation	GGTESRSSALGAADS CCCCCCHHHCCCCCC	26.80	25332170
138	Phosphorylation	LIAEGDASPGEDRTP HHEECCCCCCCCCCC	37.87	28450419
228	Ubiquitination	MLQPGVNKFSLRMFG HHCCCCCHHHHHHHC	33.64	22817900
230	Phosphorylation	QPGVNKFSLRMFGSQ CCCCCHHHHHHHCCH	19.37	25394399
236	Phosphorylation	FSLRMFGSQKAVERE HHHHHHCCHHHHHHH	19.76	-
238	Ubiquitination	LRMFGSQKAVEREQE HHHHCCHHHHHHHHH	56.98	30230243
316	Phosphorylation	DLVLNFRTGIVVEDN HHHHHCCCCEEECCC	27.42	23403867
324	Phosphorylation	GIVVEDNTEIILDPQ CEEECCCCEEEECHH	40.66	23403867
381	Ubiquitination	LRIVRFTKILSLLRL HHHHHHHHHHHHHHH	38.65	-
458	N-linked_Glycosylation	VSINNMVNNSWGKQY EEHHHHCCCCCHHHH	26.50	UniProtKB CARBOHYD
490	Phosphorylation	RQAPVGMSDVWLTML CCCCCCHHHHHHHHH	24.08	24043423
495	Phosphorylation	GMSDVWLTMLSMIVG CHHHHHHHHHHHHHH	10.60	24043423
498	Phosphorylation	DVWLTMLSMIVGATC HHHHHHHHHHHHHHH	8.81	24043423
504	Phosphorylation	LSMIVGATCYAMFIG HHHHHHHHHHHHHHH	10.50	24043423
506	Phosphorylation	MIVGATCYAMFIGHA HHHHHHHHHHHHHHH	9.21	24043423
514	Phosphorylation	AMFIGHATALIQSLD HHHHHHHHHHHHHHH	19.13	24043423
519	Phosphorylation	HATALIQSLDSSRRQ HHHHHHHHHHHHHHH	27.44	24043423
522	Phosphorylation	ALIQSLDSSRRQYQE HHHHHHHHHHHHHHH	30.91	24043423
523	Phosphorylation	LIQSLDSSRRQYQEK HHHHHHHHHHHHHHH	31.02	24043423
527	Phosphorylation	LDSSRRQYQEKYKQV HHHHHHHHHHHHHHH	19.48	-
531	Phosphorylation	RRQYQEKYKQVEQYM HHHHHHHHHHHHHHH	13.10	17977941
537	Phosphorylation	KYKQVEQYMSFHKLP HHHHHHHHHHHCCCC	4.92	-
554	Phosphorylation	TRQRIHDYYEHRYQG HHHHHHHHHHHHHCC	9.15	17977941
627	Phosphorylation	DYIIREGTIGKKMYF CEEEEECCCCCEEEE	23.08	22817900
633	Phosphorylation	GTIGKKMYFIQHGVV CCCCCEEEEEECCEE	13.35	22817900
650	Phosphorylation	LTKGNKETKLADGSY EECCCCEEECCCCCC	32.73	-
921	Phosphorylation	LGGSLSSSDSPLLTP HCCCCCCCCCCCCCC	38.44	19664995
923	Phosphorylation	GSLSSSDSPLLTPLQ CCCCCCCCCCCCCCC	21.28	32142685
935	Phosphorylation	PLQPGARSPQAAQPS CCCCCCCCCCCCCCC	22.34	30266825
942	Phosphorylation	SPQAAQPSPAPPGAR CCCCCCCCCCCCCCC	22.93	30266825
996	Phosphorylation	GPLSTPETPPRQPEP CCCCCCCCCCCCCCC	39.53	28655764
1005	Phosphorylation	PRQPEPPSLVAGASG CCCCCCCCCCCCCCC	46.31	21888424
1011	Phosphorylation	PSLVAGASGGASPVG CCCCCCCCCCCCCCC	36.52	21888424
1015	Phosphorylation	AGASGGASPVGFTPR CCCCCCCCCCCCCCC	24.32	21888424
1020	Phosphorylation	GASPVGFTPRGGLSP CCCCCCCCCCCCCCC	13.22	-
1026	Phosphorylation	FTPRGGLSPPGHSPG CCCCCCCCCCCCCCC	32.12	30266825
1031	Phosphorylation	GLSPPGHSPGPPRTF CCCCCCCCCCCCCCC	37.15	32142685
1059	Phosphorylation	LLLPPASSPPPPQVP CCCCCCCCCCCCCCC	43.46	32142685
1071	Phosphorylation	QVPQRRGTPPLTPGR CCCCCCCCCCCCCCC	21.66	24114839
1075	Phosphorylation	RRGTPPLTPGRLTQD CCCCCCCCCCCCCCC	29.48	20044836
1105	Phosphorylation	AQTLRRASPHSSGES HHHHHHHCCCCCCCC	22.72	-
1108	Phosphorylation	LRRASPHSSGESMAA HHHHCCCCCCCCCCC	43.34	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
531	Y	Phosphorylation	Kinase	SRC	P12931	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of HCN4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HCN4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HCN3_HUMAN	HCN3	physical	12928435
HCN2_HUMAN	HCN2	physical	12928435

Drug and Disease Associations

• Kegg Disease
• H00728: Brugada syndrome (BRS)
• H00729: Sick sinus syndrome (SSS); Sinus node dysfunction
• OMIM Disease
• 163800: Sick sinus syndrome 2 (SSS2)
• 613123: Brugada syndrome 8 (BRGDA8)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.