dbPTM

HCN2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	HCN2_MOUSE
UniProt AC	O88703
Protein Name	Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Gene Name	Hcn2
Organism	Mus musculus (Mouse).
Sequence Length	863
Subcellular Localization	Cell membrane Multi-pass membrane protein .
Protein Description	Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current..
Protein Sequence	MDARGGGGRPGDSPGTTPAPGPPPPPPPPAPPQPQPPPAPPPNPTTPSHPESADEPGPRARLCSRDSACTPGAAKGGANGECGRGEPQCSPEGPARGPKVSFSCRGAASGPSAAEEAGSEEAGPAGEPRGSQASFLQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPSDCWVSINNMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLSSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPQVTSAIATLQQAVAMSFCPQVARPLVGPLALGSPRLVRRAPPGPLPPAASPGPPAASPPAAPSSPRAPRTSPYGVPGSPATRVGPALPARRLSRASRPLSASQPSLPHGVPAPSPAASARPASSSTPRLGPAPTARTAAPSPDRRDSASPGAASGLDPLDSARSRLSSNL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
63	S-palmitoylation	PGPRARLCSRDSACT CCCCCCCCCCCCCCC	2.33	26546007
64	Phosphorylation	GPRARLCSRDSACTP CCCCCCCCCCCCCCC	43.65	25521595
67	Phosphorylation	ARLCSRDSACTPGAA CCCCCCCCCCCCCCC	24.92	22324799
69	S-palmitoylation	LCSRDSACTPGAAKG CCCCCCCCCCCCCCC	5.66	26546007
70	Phosphorylation	CSRDSACTPGAAKGG CCCCCCCCCCCCCCC	25.19	22817900
82	S-palmitoylation	KGGANGECGRGEPQC CCCCCCCCCCCCCCC	4.83	26546007
89	S-palmitoylation	CGRGEPQCSPEGPAR CCCCCCCCCCCCCCC	12.25	26546007
90	Phosphorylation	GRGEPQCSPEGPARG CCCCCCCCCCCCCCC	22.27	25521595
99	Ubiquitination	EGPARGPKVSFSCRG CCCCCCCCEEEEECC	54.02	-
101	Phosphorylation	PARGPKVSFSCRGAA CCCCCCEEEEECCCC	19.98	29899451
103	Phosphorylation	RGPKVSFSCRGAASG CCCCEEEEECCCCCC	8.73	22324799
104	S-palmitoylation	GPKVSFSCRGAASGP CCCEEEEECCCCCCC	4.36	26546007
109	Phosphorylation	FSCRGAASGPSAAEE EEECCCCCCCCHHHH	51.18	24925903
112	Phosphorylation	RGAASGPSAAEEAGS CCCCCCCCHHHHHCC	43.57	22324799
119	Phosphorylation	SAAEEAGSEEAGPAG CHHHHHCCCCCCCCC	39.03	25521595
131	Phosphorylation	PAGEPRGSQASFLQR CCCCCCCHHHHHHHH	24.69	25159016
134	Phosphorylation	EPRGSQASFLQRQFG CCCCHHHHHHHHHHH	20.89	25159016
150	Ubiquitination	LLQPGVNKFSLRMFG HHCCCCCHHHHHHHC	33.64	-
152	Phosphorylation	QPGVNKFSLRMFGSQ CCCCCHHHHHHHCCH	19.37	29899451
160	Ubiquitination	LRMFGSQKAVEREQE HHHHCCHHHHHHHHH	56.98	27667366
291	Ubiquitination	GIDSEVYKTARALRI CCCHHHHHHHHHHHH	40.67	27667366
380	N-linked_Glycosylation	VSINNMVNHSWSELY EEHHHHCCCCHHHHH	16.83	-
459	Phosphorylation	KYKQVEQYMSFHKLP HHHHHHHHHHHCCCC	4.92	-
464	Ubiquitination	EQYMSFHKLPADFRQ HHHHHHCCCCHHHHH	54.90	-
472	Ubiquitination	LPADFRQKIHDYYEH CCHHHHHHHHHHHHH	37.44	27667366
476	Phosphorylation	FRQKIHDYYEHRYQG HHHHHHHHHHHHHCC	9.15	22817900
510	Ubiquitination	IVNFNCRKLVASMPL HCCCCCCHHHHHCCC	49.90	-
514	Phosphorylation	NCRKLVASMPLFANA CCCHHHHHCCCCCCC	17.29	26160508
527	Phosphorylation	NADPNFVTAMLTKLK CCCCCHHHHHHHHCE	11.38	26160508
531	Phosphorylation	NFVTAMLTKLKFEVF CHHHHHHHHCEEEEE	22.65	26160508
639	Ubiquitination	RLDRIGKKNSILLHK HHHHHCCCCCCCEEE	51.19	27667366
641	Phosphorylation	DRIGKKNSILLHKVQ HHHCCCCCCCEEECC	24.40	22324799
652	Phosphorylation	HKVQHDLSSGVFNNQ EECCCCCCCCCCCCH	30.97	29899451
653	Phosphorylation	KVQHDLSSGVFNNQE ECCCCCCCCCCCCHH	46.39	29899451
726	Phosphorylation	VGPLALGSPRLVRRA CCCHHCCCCCCCCCC	14.01	22817900
728	Methylation	PLALGSPRLVRRAPP CHHCCCCCCCCCCCC	47.73	24129315
743	Phosphorylation	GPLPPAASPGPPAAS CCCCCCCCCCCCCCC	32.28	24925903
750	Phosphorylation	SPGPPAASPPAAPSS CCCCCCCCCCCCCCC	33.26	25521595
756	Phosphorylation	ASPPAAPSSPRAPRT CCCCCCCCCCCCCCC	48.53	25521595
757	Phosphorylation	SPPAAPSSPRAPRTS CCCCCCCCCCCCCCC	19.83	25521595
763	Phosphorylation	SSPRAPRTSPYGVPG CCCCCCCCCCCCCCC	32.75	20047950
764	Phosphorylation	SPRAPRTSPYGVPGS CCCCCCCCCCCCCCC	19.87	25521595
766	Phosphorylation	RAPRTSPYGVPGSPA CCCCCCCCCCCCCCC	30.44	24925903
771	Phosphorylation	SPYGVPGSPATRVGP CCCCCCCCCCCCCCC	12.80	25521595
774	Phosphorylation	GVPGSPATRVGPALP CCCCCCCCCCCCCCC	29.31	24925903
789	Phosphorylation	ARRLSRASRPLSASQ HHHHHCCCCCCCCCC	33.06	29899451
793	Phosphorylation	SRASRPLSASQPSLP HCCCCCCCCCCCCCC	28.67	29899451
795	Phosphorylation	ASRPLSASQPSLPHG CCCCCCCCCCCCCCC	38.97	20047950
798	Phosphorylation	PLSASQPSLPHGVPA CCCCCCCCCCCCCCC	47.07	29899451
807	Phosphorylation	PHGVPAPSPAASARP CCCCCCCCCCHHCCC	29.31	19060867
811	Phosphorylation	PAPSPAASARPASSS CCCCCCHHCCCCCCC	27.24	20415495
816	Phosphorylation	AASARPASSSTPRLG CHHCCCCCCCCCCCC	27.75	29899451
817	Phosphorylation	ASARPASSSTPRLGP HHCCCCCCCCCCCCC	40.10	22324799
818	Phosphorylation	SARPASSSTPRLGPA HCCCCCCCCCCCCCC	39.68	22324799
819	Phosphorylation	ARPASSSTPRLGPAP CCCCCCCCCCCCCCC	17.86	29899451
827	Phosphorylation	PRLGPAPTARTAAPS CCCCCCCCCCCCCCC	30.77	29899451
830	Phosphorylation	GPAPTARTAAPSPDR CCCCCCCCCCCCCCC	25.38	22324799
834	Phosphorylation	TARTAAPSPDRRDSA CCCCCCCCCCCCCCC	34.40	25521595
840	Phosphorylation	PSPDRRDSASPGAAS CCCCCCCCCCCCHHC	28.91	25521595
842	Phosphorylation	PDRRDSASPGAASGL CCCCCCCCCCHHCCC	28.02	25521595
847	Phosphorylation	SASPGAASGLDPLDS CCCCCHHCCCCHHHH	39.08	25521595
860	Phosphorylation	DSARSRLSSNL---- HHHHHHHHHCC----	18.97	22807455

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
476	Y	Phosphorylation	Kinase	SRC	P12931	PSP
641	S	Phosphorylation	Kinase	PRKG2	Q13237	GPS
641	S	Phosphorylation	Kinase	PRKG2	Q61410	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
641	S	Phosphorylation		21347269
Phosphorylation at Ser-641 by PRKG2 shifts the voltage-dependence to more negative voltages, hence counteracting the stimulatory effect of cGMP on gating.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of HCN2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
HCN1_MOUSE	Hcn1	physical	12928435

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of HCN2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119; SER-750 ANDSER-771, AND MASS SPECTROMETRY.	17114649 [Abstract]

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