dbPTM

H33_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	H33_RAT
UniProt AC	P84245
Protein Name	Histone H3.3
Gene Name	H3f3b
Organism	Rattus norvegicus (Rat).
Sequence Length	136
Subcellular Localization	Nucleus. Chromosome.
Protein Description	Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling..
Protein Sequence	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Asymmetric dimethylarginine	-----MARTKQTARK -----CCCCCHHHHH	40.95	-
3	Citrullination	-----MARTKQTARK -----CCCCCHHHHH	40.95	-
3	Methylation	-----MARTKQTARK -----CCCCCHHHHH	40.95	-
4	Phosphorylation	----MARTKQTARKS ----CCCCCHHHHHC	20.26	11498542
5	Allysine	---MARTKQTARKST ---CCCCCHHHHHCC	39.85	-
5	Acetylation	---MARTKQTARKST ---CCCCCHHHHHCC	39.85	1001435
5	Crotonylation	---MARTKQTARKST ---CCCCCHHHHHCC	39.85	-
5	Deamination	---MARTKQTARKST ---CCCCCHHHHHCC	39.85	-
5	Methylation	---MARTKQTARKST ---CCCCCHHHHHCC	39.85	-
5	Other	---MARTKQTARKST ---CCCCCHHHHHCC	39.85	-
6	Formation of an isopeptide bond	--MARTKQTARKSTG --CCCCCHHHHHCCC	39.52	32273471
6	Serotonylation	--MARTKQTARKSTG --CCCCCHHHHHCCC	39.52	-
7	Phosphorylation	-MARTKQTARKSTGG -CCCCCHHHHHCCCC	29.95	-
9	Citrullination	ARTKQTARKSTGGKA CCCCHHHHHCCCCCC	36.52	-
9	Citrullination	ARTKQTARKSTGGKA CCCCHHHHHCCCCCC	36.52	-
9	Methylation	ARTKQTARKSTGGKA CCCCHHHHHCCCCCC	36.52	-
10	"N6,N6,N6-trimethyllysine"	RTKQTARKSTGGKAP CCCHHHHHCCCCCCC	50.32	-
10	Acetylation	RTKQTARKSTGGKAP CCCHHHHHCCCCCCC	50.32	25786129
10	Crotonylation	RTKQTARKSTGGKAP CCCHHHHHCCCCCCC	50.32	-
10	Lactoylation	RTKQTARKSTGGKAP CCCHHHHHCCCCCCC	50.32	-
10	Methylation	RTKQTARKSTGGKAP CCCHHHHHCCCCCCC	50.32	-
10	Other	RTKQTARKSTGGKAP CCCHHHHHCCCCCCC	50.32	-
11	ADP-ribosylation	TKQTARKSTGGKAPR CCHHHHHCCCCCCCH	27.08	-
11	Phosphorylation	TKQTARKSTGGKAPR CCHHHHHCCCCCCCH	27.08	10911986
12	Phosphorylation	KQTARKSTGGKAPRK CHHHHHCCCCCCCHH	53.65	12560483
15	Acetylation	ARKSTGGKAPRKQLA HHHCCCCCCCHHHHH	57.47	25786129
15	Glutarylation	ARKSTGGKAPRKQLA HHHCCCCCCCHHHHH	57.47	-
15	Lactoylation	ARKSTGGKAPRKQLA HHHCCCCCCCHHHHH	57.47	-
15	Other	ARKSTGGKAPRKQLA HHHCCCCCCCHHHHH	57.47	-
15	Succinylation	ARKSTGGKAPRKQLA HHHCCCCCCCHHHHH	57.47	-
18	Asymmetric dimethylarginine	STGGKAPRKQLATKA CCCCCCCHHHHHHHH	44.18	-
18	Citrullination	STGGKAPRKQLATKA CCCCCCCHHHHHHHH	44.18	-
18	Methylation	STGGKAPRKQLATKA CCCCCCCHHHHHHHH	44.18	-
19	N6-crotonyl-L-lysine	TGGKAPRKQLATKAA CCCCCCHHHHHHHHH	48.91	-
19	Acetylation	TGGKAPRKQLATKAA CCCCCCHHHHHHHHH	48.91	25786129
19	Butyrylation	TGGKAPRKQLATKAA CCCCCCHHHHHHHHH	48.91	-
19	Crotonylation	TGGKAPRKQLATKAA CCCCCCHHHHHHHHH	48.91	-
19	Glutarylation	TGGKAPRKQLATKAA CCCCCCHHHHHHHHH	48.91	-
19	Lactoylation	TGGKAPRKQLATKAA CCCCCCHHHHHHHHH	48.91	-
19	Methylation	TGGKAPRKQLATKAA CCCCCCHHHHHHHHH	48.91	-
19	Other	TGGKAPRKQLATKAA CCCCCCHHHHHHHHH	48.91	-
24	N6-crotonyl-L-lysine	PRKQLATKAARKSAP CHHHHHHHHHHHHCC	34.15	-
24	Acetylation	PRKQLATKAARKSAP CHHHHHHHHHHHHCC	34.15	25786129
24	Butyrylation	PRKQLATKAARKSAP CHHHHHHHHHHHHCC	34.15	-
24	Crotonylation	PRKQLATKAARKSAP CHHHHHHHHHHHHCC	34.15	-
24	Glutarylation	PRKQLATKAARKSAP CHHHHHHHHHHHHCC	34.15	-
24	Lactoylation	PRKQLATKAARKSAP CHHHHHHHHHHHHCC	34.15	-
24	Methylation	PRKQLATKAARKSAP CHHHHHHHHHHHHCC	34.15	-
24	Other	PRKQLATKAARKSAP CHHHHHHHHHHHHCC	34.15	-
27	Citrullination	QLATKAARKSAPSTG HHHHHHHHHHCCCCC	38.02	-
27	Citrullination	QLATKAARKSAPSTG HHHHHHHHHHCCCCC	38.02	-
28	"N6,N6,N6-trimethyllysine"	LATKAARKSAPSTGG HHHHHHHHHCCCCCC	46.36	-
28	Acetylation	LATKAARKSAPSTGG HHHHHHHHHCCCCCC	46.36	25786129
28	Crotonylation	LATKAARKSAPSTGG HHHHHHHHHCCCCCC	46.36	-
28	Glutarylation	LATKAARKSAPSTGG HHHHHHHHHCCCCCC	46.36	-
28	Lactoylation	LATKAARKSAPSTGG HHHHHHHHHCCCCCC	46.36	-
28	Methylation	LATKAARKSAPSTGG HHHHHHHHHCCCCCC	46.36	-
28	Other	LATKAARKSAPSTGG HHHHHHHHHCCCCCC	46.36	-
29	ADP-ribosylation	ATKAARKSAPSTGGV HHHHHHHHCCCCCCC	39.60	-
29	Phosphorylation	ATKAARKSAPSTGGV HHHHHHHHCCCCCCC	39.60	28432305
32	Phosphorylation	AARKSAPSTGGVKKP HHHHHCCCCCCCCCC	39.13	-
37	"N6,N6,N6-trimethyllysine"	APSTGGVKKPHRYRP CCCCCCCCCCCCCCC	64.95	-
37	Acetylation	APSTGGVKKPHRYRP CCCCCCCCCCCCCCC	64.95	19343714
37	Methylation	APSTGGVKKPHRYRP CCCCCCCCCCCCCCC	64.95	-
37	Other	APSTGGVKKPHRYRP CCCCCCCCCCCCCCC	64.95	-
38	Acetylation	PSTGGVKKPHRYRPG CCCCCCCCCCCCCCC	43.58	25786129
38	Methylation	PSTGGVKKPHRYRPG CCCCCCCCCCCCCCC	43.58	-
42	Phosphorylation	GVKKPHRYRPGTVAL CCCCCCCCCCCCHHH	20.37	-
46	Phosphorylation	PHRYRPGTVALREIR CCCCCCCCHHHHHHH	12.80	23984901
57	"N6,N6,N6-trimethyllysine"	REIRRYQKSTELLIR HHHHHHHHCHHHHHH	50.87	-
57	Acetylation	REIRRYQKSTELLIR HHHHHHHHCHHHHHH	50.87	22902405
57	Crotonylation	REIRRYQKSTELLIR HHHHHHHHCHHHHHH	50.87	-
57	Glutarylation	REIRRYQKSTELLIR HHHHHHHHCHHHHHH	50.87	-
57	Lactoylation	REIRRYQKSTELLIR HHHHHHHHCHHHHHH	50.87	-
57	Methylation	REIRRYQKSTELLIR HHHHHHHHCHHHHHH	50.87	-
57	Other	REIRRYQKSTELLIR HHHHHHHHCHHHHHH	50.87	-
57	Succinylation	REIRRYQKSTELLIR HHHHHHHHCHHHHHH	50.87	-
58	Phosphorylation	EIRRYQKSTELLIRK HHHHHHHCHHHHHHH	16.16	27097102
59	Phosphorylation	IRRYQKSTELLIRKL HHHHHHCHHHHHHHC	37.37	27097102
65	Methylation	STELLIRKLPFQRLV CHHHHHHHCCHHHHH	54.22	-
65	Other	STELLIRKLPFQRLV CHHHHHHHCCHHHHH	54.22	-
65	Succinylation	STELLIRKLPFQRLV CHHHHHHHCCHHHHH	54.22	26843850
80	"N6,N6,N6-trimethyllysine"	REIAQDFKTDLRFQS HHHHHHHHHCHHHHH	49.79	-
80	Acetylation	REIAQDFKTDLRFQS HHHHHHHHHCHHHHH	49.79	22902405
80	Glutarylation	REIAQDFKTDLRFQS HHHHHHHHHCHHHHH	49.79	-
80	Lactoylation	REIAQDFKTDLRFQS HHHHHHHHHCHHHHH	49.79	-
80	Methylation	REIAQDFKTDLRFQS HHHHHHHHHCHHHHH	49.79	-
80	Other	REIAQDFKTDLRFQS HHHHHHHHHCHHHHH	49.79	-
80	Succinylation	REIAQDFKTDLRFQS HHHHHHHHHCHHHHH	49.79	-
80	Ubiquitination	REIAQDFKTDLRFQS HHHHHHHHHCHHHHH	49.79	-
81	Phosphorylation	EIAQDFKTDLRFQSA HHHHHHHHCHHHHHH	40.01	29779826
87	Phosphorylation	KTDLRFQSAAIGALQ HHCHHHHHHHHHHHH	19.56	16641100
97	Phosphorylation	IGALQEASEAYLVGL HHHHHHHHHHHHHHH	23.23	17683130
108	Phosphorylation	LVGLFEDTNLCAIHA HHHHHCCCCEEEEEE	23.93	-
116	Acetylation	NLCAIHAKRVTIMPK CEEEEEEEECEECHH	32.99	25786129
116	Glutarylation	NLCAIHAKRVTIMPK CEEEEEEEECEECHH	32.99	-
123	Acetylation	KRVTIMPKDIQLARR EECEECHHHHHHHHH	50.51	-
123	Glutarylation	KRVTIMPKDIQLARR EECEECHHHHHHHHH	50.51	-
123	Methylation	KRVTIMPKDIQLARR EECEECHHHHHHHHH	50.51	-
123	Other	KRVTIMPKDIQLARR EECEECHHHHHHHHH	50.51	-
123	Succinylation	KRVTIMPKDIQLARR EECEECHHHHHHHHH	50.51	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
4	T	Phosphorylation	Kinase	HASPIN	-	Uniprot
7	T	Phosphorylation	Kinase	PKC	-	Uniprot
11	S	Phosphorylation	Kinase	RPS6KA5	A0A0G2K366	GPS
11	S	Phosphorylation	Kinase	AURKB	O55099	GPS
11	S	Phosphorylation	Kinase	RPS6KA3	D3Z8E0	GPS
11	S	Phosphorylation	Kinase	RPS6KA4	D3ZSB7	GPS
11	S	Phosphorylation	Kinase	AURKC	D4AD76	GPS
11	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
11	S	Phosphorylation	Kinase	ALTERNATE	-	Uniprot
12	T	Phosphorylation	Kinase	DAPK3	O88764	GPS
12	T	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
12	T	Phosphorylation	Kinase	PKC	-	Uniprot
29	S	Phosphorylation	Kinase	MAPK8	P49185	GPS
29	S	Phosphorylation	Kinase	MAPK9	P49186	GPS
29	S	Phosphorylation	Kinase	ALTERNATE	-	Uniprot
46	T	Phosphorylation	Kinase	DYRK1A	Q63470	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
3	R	Methylation	-
Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3).
4	T	Phosphorylation	-
Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase.
5	K	ubiquitylation	-
Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'.
5	K	Acetylation	-
Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4.
5	K	Methylation	-
Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3).
5	K	Methylation	-
H3Q5ser is associated with trimethylation of Lys-5 (H3K4me3) and enhances general transcription factor IID (TFIID) complex-binding to H3K4me3, thereby facilitating transcription (By similarity).
5	K	Methylation	-
Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'.
5	K	Methylation	-
Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4.
5	K	Phosphorylation	-
Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A.
5	K	Methylation	-
Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80.
5	K	Methylation	-
Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A.
7	T	Phosphorylation	-
Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A.
7	T	Methylation	-
Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A.
9	R	Methylation	-
Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression.
9	R	Methylation	-
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
9	R	Methylation	-
Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s).
9	R	Citrullination	-
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
9	R	Acetylation	-
Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s).
10	K	Phosphorylation	-
Serine ADP-ribosylation at Ser-11 (H3S10ADPr) is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac).
10	K	Phosphorylation	-
Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C.
10	K	Phosphorylation	-
Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4.
10	K	Methylation	-
Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C.
10	K	Methylation	-
Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4.
10	K	Phosphorylation	-
Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4.
10	K	Acetylation	-
Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s).
10	K	Acetylation	-
Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4.
10	K	Acetylation	-
Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4.
10	K	Acetylation	-
Serine ADP-ribosylation at Ser-11 (H3S10ADPr) is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac).
10	K	Methylation	-
Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s).
10	K	Methylation	-
Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
10	K	Methylation	-
Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented.
10	K	Methylation	-
Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4.
11	S	Acetylation	12560483
Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4.
11	S	Acetylation	12560483
Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4.
11	S	Acetylation	12560483
Serine ADP-ribosylation at Ser-11 (H3S10ADPr) is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac).
11	S	Methylation	12560483
Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4.
11	S	Methylation	12560483
Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4.
11	S	Phosphorylation	12560483
In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun.
11	S	Phosphorylation	12560483
Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4.
11	S	Phosphorylation	12560483
Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis.
11	S	Phosphorylation	12560483
Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
11	S	Phosphorylation	12560483
Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation.
11	S	Phosphorylation	12560483
Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4.
11	S	Phosphorylation	12560483
Serine ADP-ribosylation at Ser-11 (H3S10ADPr) is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac).
12	T	Phosphorylation	12560483
Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C.
12	T	Methylation	12560483
Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C.
12	T	Phosphorylation	12560483
At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1.
18	R	Citrullination	-
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
18	R	Acetylation	-
Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
18	R	Methylation	-
Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
18	R	Methylation	-
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation.
18	R	Methylation	-
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
19	K	Methylation	-
Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
19	K	Acetylation	-
Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
24	K	Methylation	-
Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
24	K	Acetylation	-
Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
28	K	Methylation	-
Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented.
28	K	Methylation	-
Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
29	S	Phosphorylation	-
Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation.
32	S	Phosphorylation	-
Phosphorylation on Ser-32 (H3S31ph) is specific to regions bordering centromeres in metaphase chromosomes.
37	K	Methylation	19343714
Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80.
57	K	Methylation	-
Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.
80	K	Succinylation	-
Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes.
80	K	ubiquitylation	-
Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'.
80	K	Methylation	-
Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1.
80	K	Methylation	-
Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'.
80	K	Methylation	-
Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80.
120	K	Methylation	-
Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'.
120	K	ubiquitylation	-
Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'.
123	K	Acetylation	-
Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.
123	K	Succinylation	-
Desuccinylation at Lys-123 (H3K122succ) by SIRT7 in response to DNA damage promotes chromatin condensation and double-strand breaks (DSBs) repair.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of H33_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
H33_RAT	H3f3b	physical	22371606

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of H33_RAT

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Mass spectrometry-compatible silver staining of histones resolved onacetic acid-urea-Triton PAGE."; Pramod K.S., Bharat K., Sanjay G.; Proteomics 9:2589-2592(2009). Cited for: IDENTIFICATION BY MASS SPECTROMETRY, AND ACETYLATION AT LYS-37.	19343714 [Abstract]
Phosphorylation
Reference	PubMed
"Novel mitosis-specific phosphorylation of histone H3 at Thr11mediated by Dlk/ZIP kinase."; Preuss U., Landsberg G., Scheidtmann K.H.; Nucleic Acids Res. 31:878-885(2003). Cited for: PHOSPHORYLATION AT SER-11 AND THR-12.	12560483 [Abstract]

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