GFI1B_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GFI1B_MOUSE
• UniProt AC: O70237
• Protein Name: Zinc finger protein Gfi-1b
• Gene Name: Gfi1b
• Organism: Mus musculus (Mouse).
• Sequence Length: 330
• Subcellular Localization: Nucleus . Localized at foci of pericentric heterochromatin.
• Protein Description: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation..
• Protein Sequence: MPRSFLVKSKKAHTYHQPRAQGDELVWPPAVIPVAKEHSQSASPLLSTPLPSQTLDWNTIKQEREMLLNQSLPKMASAPEGPLVTPQPQDGESPLSESPPFYKPSFSWDTLASSYSHSYTQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFRLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDVCGKTFGHAVSLEQHTHVHSQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8	"N6,N6-dimethyllysine"	MPRSFLVKSKKAHTY CCCCEEECCCCCCCC	59.72	-
8	Methylation	MPRSFLVKSKKAHTY CCCCEEECCCCCCCC	59.72	-
39	Phosphorylation	IPVAKEHSQSASPLL EEHHHHHCCCCCCCC	27.24	26745281
41	Phosphorylation	VAKEHSQSASPLLST HHHHHCCCCCCCCCC	33.46	26745281
43	Phosphorylation	KEHSQSASPLLSTPL HHHCCCCCCCCCCCC	22.71	26745281
47	Phosphorylation	QSASPLLSTPLPSQT CCCCCCCCCCCCCCC	35.34	21183079
48	Phosphorylation	SASPLLSTPLPSQTL CCCCCCCCCCCCCCC	28.90	21082442
71	Phosphorylation	REMLLNQSLPKMASA HHHHHHCCCCCCCCC	45.63	30482847
140	Phosphorylation	RSVRLYGSPLVPSTE HHHHHHCCCCCCCCC	11.08	26745281
145	Phosphorylation	YGSPLVPSTESPLDF HCCCCCCCCCCCCCE	37.01	19854140
146	Phosphorylation	GSPLVPSTESPLDFR CCCCCCCCCCCCCEE	34.12	21082442
148	Phosphorylation	PLVPSTESPLDFRLR CCCCCCCCCCCEECC	30.74	22817900
156	Phosphorylation	PLDFRLRYSPGMDTY CCCEECCCCCCCCEE	24.65	26745281
157	Phosphorylation	LDFRLRYSPGMDTYH CCEECCCCCCCCEEE	14.45	26745281
299	Phosphorylation	ITHSRKHTGFKPFSC CCCCCCCCCCCCEEE	47.55	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of GFI1B_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
8	K	Methylation	Methylation at Lys-8 in the SNAG domain seems required for the recruitment of the corepressor complex.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GFI1B_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KDM1A_MOUSE	Kdm1a	physical	17707228
HDAC2_MOUSE	Hdac2	physical	17707228
HDAC1_MOUSE	Hdac1	physical	17707228
RCOR3_MOUSE	Rcor3	physical	17707228
RCOR2_MOUSE	Rcor2	physical	17707228

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.