FAD1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: FAD1_HUMAN
• UniProt AC: Q8NFF5
• Protein Name: FAD synthase
• Gene Name: FLAD1
• Organism: Homo sapiens (Human).
• Sequence Length: 587
• Subcellular Localization: Isoform 1: Mitochondrion matrix .; Isoform 2: Cytoplasm.
• Protein Description: Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme..
• Protein Sequence: MGWDLGTRLFQRQEQRSRLSRIWLEKTRVFLEGSTRTPALPHCLFWLLQVPSTQDPLFPGYGPQCPVDLAGPPCLRPLFGGLGGYWRALQRGREGRTMTSRASELSPGRSVTAGIIIVGDEILKGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAVQRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRRTDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRGYTSLGSRENTVRNPALKCLSPGGHPTYRPAYLLENEEEERNSRT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9 (in isoform 3)	Phosphorylation	-	4.27	25849741
9 (in isoform 2)	Phosphorylation	-	4.27	25849741
20	Phosphorylation	QEQRSRLSRIWLEKT HHHHHHHHHHHHHHH	22.17	28348404
27	Phosphorylation	SRIWLEKTRVFLEGS HHHHHHHHEEECCCC	23.77	28348404
99	Phosphorylation	GREGRTMTSRASELS CCCCCCCCCCHHHCC	17.91	22167270
100	Phosphorylation	REGRTMTSRASELSP CCCCCCCCCHHHCCC	18.28	22167270
103	Phosphorylation	RTMTSRASELSPGRS CCCCCCHHHCCCCCC	37.65	22167270
106	Phosphorylation	TSRASELSPGRSVTA CCCHHHCCCCCCEEE	22.66	29255136
110	Phosphorylation	SELSPGRSVTAGIII HHCCCCCCEEEEEEE	29.72	24247654
111	Ubiquitination	ELSPGRSVTAGIIIV HCCCCCCEEEEEEEE	4.02	29967540
113	Ubiquitination	SPGRSVTAGIIIVGD CCCCCEEEEEEEECC	12.67	29967540
120	Ubiquitination	AGIIIVGDEILKGHT EEEEEECCHHHCCCC	28.95	27667366
122	Ubiquitination	IIIVGDEILKGHTQD EEEECCHHHCCCCCC	5.99	27667366
141	Phosphorylation	FLCRTLRSLGVQVCR HHHHHHHHCCCEEEE	32.37	20068231
210	Ubiquitination	PKLEAATKALGGEGW HHHHHHHHHHCCCCH	36.57	29967540
219	Ubiquitination	LGGEGWEKLSLVPSS HCCCCHHHEEECCCC	36.55	27667366
247	Phosphorylation	PFRFPLVSVRNVYLF CEEECEEEEECEEEC	24.04	24719451
281	Ubiquitination	NPAVQFHSKELYVAA CCHHEECCCEEEEEE	29.16	29967540
285 (in isoform 5)	Phosphorylation	-	6.80	25072903
288 (in isoform 5)	Phosphorylation	-	12.72	25072903
291 (in isoform 5)	Phosphorylation	-	13.54	25072903
294 (in isoform 5)	Phosphorylation	-	5.21	25072903
296 (in isoform 5)	Phosphorylation	-	4.15	25072903
299 (in isoform 5)	Phosphorylation	-	37.56	25072903
301 (in isoform 5)	Phosphorylation	-	27.20	25072903
372	Phosphorylation	AVYKLAESGSSLGKK HHHHHHHHCCHHHHH	38.07	22985185
378	Succinylation	ESGSSLGKKVAGALQ HHCCHHHHHHHHHHH	49.74	-
378	Ubiquitination	ESGSSLGKKVAGALQ HHCCHHHHHHHHHHH	49.74	29967540
378	2-Hydroxyisobutyrylation	ESGSSLGKKVAGALQ HHCCHHHHHHHHHHH	49.74	-
378	Acetylation	ESGSSLGKKVAGALQ HHCCHHHHHHHHHHH	49.74	-
378	Succinylation	ESGSSLGKKVAGALQ HHCCHHHHHHHHHHH	49.74	-
440	Phosphorylation	ILYIRSISPFPELEQ EEEEEECCCCHHHHH	23.02	-
466	Phosphorylation	QMLEAEGSMKQALGE HHHHHCCHHHHHHHH	17.82	18691976
553	Phosphorylation	SLGSRENTVRNPALK CCCCCCCCCCCCHHH	18.98	26074081
563	Phosphorylation	NPALKCLSPGGHPTY CCHHHCCCCCCCCCC	30.85	22167270
569	Phosphorylation	LSPGGHPTYRPAYLL CCCCCCCCCCCHHHC	26.87	28450419
570	Phosphorylation	SPGGHPTYRPAYLLE CCCCCCCCCCHHHCC	21.03	28450419
574	Phosphorylation	HPTYRPAYLLENEEE CCCCCCHHHCCCHHH	17.95	28450419

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of FAD1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of FAD1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of FAD1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FAD1_HUMAN	FLAD1	physical	16189514
GEMI4_HUMAN	GEMIN4	physical	21988832
FAD1_HUMAN	FLAD1	physical	25416956
PUR4_HUMAN	PFAS	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND SER-563, ANDMASS SPECTROMETRY.
PubMed: 19369195

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, AND MASSSPECTROMETRY.
PubMed: 18669648

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND SER-563, ANDMASS SPECTROMETRY.
PubMed: 18691976

"A probability-based approach for high-throughput proteinphosphorylation analysis and site localization.";
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
Nat. Biotechnol. 24:1285-1292(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, AND MASSSPECTROMETRY.
PubMed: 16964243