dbPTM

EAA1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	EAA1_MOUSE
UniProt AC	P56564
Protein Name	Excitatory amino acid transporter 1
Gene Name	Slc1a3
Organism	Mus musculus (Mouse).
Sequence Length	543
Subcellular Localization	Cell membrane Multi-pass membrane protein .
Protein Description	Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. [PubMed: 7903437]
Protein Sequence	MTKSNGEEPRMGGRMERLQQGVRKRTLLAKKKVQSLTKEDVKSYLFRNAFVLLTVTAVIVGTILGFALRPYKMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGIIIVIIIHPGKGTKENMYREGKIVQVTAADAFLDLIRNMFPPNLVEACFKQFKTSYEKRSFKVPIQSNETLLGAVINNVSEAMETLTRIREEMVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMRLVAVIMWYAPLGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLPVGATINMDGTALYEALAAIFIAQVNNFDLNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNEPEKPVADSETKM

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Acetylation	-----MTKSNGEEPR -----CCCCCCCCCC	40.40	19857865
4	Phosphorylation	----MTKSNGEEPRM ----CCCCCCCCCCC	40.68	25521595
26	Phosphorylation	QQGVRKRTLLAKKKV HHHHHHHHHHHHHHH	29.20	29899451
35	Phosphorylation	LAKKKVQSLTKEDVK HHHHHHHHCCHHHHH	40.78	22817900
42	Ubiquitination	SLTKEDVKSYLFRNA HCCHHHHHHHHHHHH	45.78	22790023
206	N-linked_Glycosylation	FKVPIQSNETLLGAV EECCCCCCCHHHHHH	29.87	19656770
215	N-linked_Glycosylation	TLLGAVINNVSEAME HHHHHHHHCHHHHHH	35.93	19656770
216	N-linked_Glycosylation	LLGAVINNVSEAMET HHHHHHHCHHHHHHH	27.80	19656770
384	Ubiquitination	EENNGVDKRITRFVL HHCCCCCCCEEEEEE	43.20	22790023
503	Ubiquitination	HLSRHELKNRDVEMG HHHHHHHHCCCCCCC	47.13	22790023
512	Phosphorylation	RDVEMGNSVIEENEM CCCCCCCCHHCCCCC	20.81	25521595
520	Ubiquitination	VIEENEMKKPYQLIA HHCCCCCCCCEEEEC	44.40	22790023
521	Ubiquitination	IEENEMKKPYQLIAQ HCCCCCCCCEEEECC	47.88	22790023
523	Phosphorylation	ENEMKKPYQLIAQDN CCCCCCCEEEECCCC	25.49	29550500
534	Ubiquitination	AQDNEPEKPVADSET CCCCCCCCCCCCCCC	56.35	22790023
539	Phosphorylation	PEKPVADSETKM--- CCCCCCCCCCCC---	37.11	25521595
541	Phosphorylation	KPVADSETKM----- CCCCCCCCCC-----	36.04	29899451
542	Ubiquitination	PVADSETKM------ CCCCCCCCC------	38.26	22790023

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of EAA1_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of EAA1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of EAA1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
SEPT4_MOUSE	Sept4	physical	14723703

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of EAA1_MOUSE

Related Literatures of Post-Translational Modification

N-linked Glycosylation
Reference	PubMed
"The mouse C2C12 myoblast cell surface N-linked glycoproteome:identification, glycosite occupancy, and membrane orientation."; Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I.,Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E.,Wollscheid B.; Mol. Cell. Proteomics 8:2555-2569(2009). Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206; ASN-215 AND ASN-216,AND MASS SPECTROMETRY.	19656770 [Abstract]
Phosphorylation
Reference	PubMed
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512, AND MASSSPECTROMETRY.	17114649 [Abstract]