dbPTM

DNJC5_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	DNJC5_MOUSE
UniProt AC	P60904
Protein Name	DnaJ homolog subfamily C member 5 {ECO:0000305}
Gene Name	Dnajc5 {ECO:0000312\|MGI:MGI:892995}
Organism	Mus musculus (Mouse).
Sequence Length	198
Subcellular Localization	Membrane Lipid-anchor . Melanosome . Cell membrane . Colocalizes with insulin granules, when overexpressed in a islet cell line.
Protein Description	Acts as a co-chaperone for the SNARE protein SNAP-25. [PubMed: 22187053 Involved in the calcium-mediated control of a late stage of exocytosis]
Protein Sequence	MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVVCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8	Phosphorylation	MADQRQRSLSTSGES CCHHHHHHCCCCCCH	20.52	25521595
10	Phosphorylation	DQRQRSLSTSGESLY HHHHHHCCCCCCHHH	23.05	24925903
11	Phosphorylation	QRQRSLSTSGESLYH HHHHHCCCCCCHHHH	46.15	25521595
12	Phosphorylation	RQRSLSTSGESLYHV HHHHCCCCCCHHHHH	36.87	25521595
15	Phosphorylation	SLSTSGESLYHVLGL HCCCCCCHHHHHHCC	37.44	25521595
17	Phosphorylation	STSGESLYHVLGLDK CCCCCHHHHHHCCCC	10.12	24925903
27	Phosphorylation	LGLDKNATSDDIKKS HCCCCCCCHHHHHHH	41.92	29899451
28	Phosphorylation	GLDKNATSDDIKKSY CCCCCCCHHHHHHHH	30.76	25367039
32	Ubiquitination	NATSDDIKKSYRKLA CCCHHHHHHHHHHHH	42.53	27667366
33	Ubiquitination	ATSDDIKKSYRKLAL CCHHHHHHHHHHHHH	53.72	27667366
41	Ubiquitination	SYRKLALKYHPDKNP HHHHHHHHHCCCCCC	35.59	27667366
46	Ubiquitination	ALKYHPDKNPDNPEA HHHHCCCCCCCCHHH	74.88	27667366
56	Ubiquitination	DNPEAADKFKEINNA CCHHHHHHHHHHHHH	54.32	27667366
56	Acetylation	DNPEAADKFKEINNA CCHHHHHHHHHHHHH	54.32	-
58	Ubiquitination	PEAADKFKEINNAHA HHHHHHHHHHHHHHH	64.52	27667366
72	Ubiquitination	AILTDATKRNIYDKY HHCCHHHHCCHHHHH	45.21	27667366
146	Phosphorylation	KAPEGEETEFYVSPE CCCCCCCCEEEECHH	27.39	25159016
149	Phosphorylation	EGEETEFYVSPEDLE CCCCCEEEECHHHHH	8.19	25159016
151	Phosphorylation	EETEFYVSPEDLEAQ CCCEEEECHHHHHHH	15.93	25521595
161	Phosphorylation	DLEAQLQSDEREATD HHHHHHHCCCCCCCC	50.46	25159016
167	Phosphorylation	QSDEREATDTPIVIQ HCCCCCCCCCCEEEE	35.34	28066266
169	Phosphorylation	DEREATDTPIVIQPA CCCCCCCCCEEEECC	15.57	28066266
177	Phosphorylation	PIVIQPASATETTQL CEEEECCCCCCCEEE	42.27	25619855
185	Phosphorylation	ATETTQLTADSHPSY CCCCEEECCCCCCCC	20.71	25619855
188	Phosphorylation	TTQLTADSHPSYHTD CEEECCCCCCCCCCC	34.53	25619855
191	Phosphorylation	LTADSHPSYHTDGFN ECCCCCCCCCCCCCC	24.91	25619855
192	Phosphorylation	TADSHPSYHTDGFN- CCCCCCCCCCCCCC-	17.12	25619855
194	Phosphorylation	DSHPSYHTDGFN--- CCCCCCCCCCCC---	29.59	25619855

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of DNJC5_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
10	S	Phosphorylation	19131326
Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58 (By similarity).
58	K	Phosphorylation	20847230
Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58 (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of DNJC5_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SNP25_MOUSE	Snap25	physical	21151134
HSP7C_MOUSE	Hspa8	physical	21151134
DNJC5_MOUSE	Dnajc5	physical	21151134
SGTA_MOUSE	Sgta	physical	21151134

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of DNJC5_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry."; Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.; Mol. Cell. Proteomics 8:904-912(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, AND MASSSPECTROMETRY.	19131326 [Abstract]
"Specific phosphopeptide enrichment with immobilized titanium ionaffinity chromatography adsorbent for phosphoproteome analysis."; Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.; J. Proteome Res. 7:3957-3967(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8, AND MASSSPECTROMETRY.	18630941 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-12 AND SER-15,AND MASS SPECTROMETRY.	17242355 [Abstract]
"Mitochondrial phosphoproteome revealed by an improved IMAC method andMS/MS/MS."; Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y.; Mol. Cell. Proteomics 6:669-676(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, AND MASSSPECTROMETRY.	17208939 [Abstract]
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10 AND THR-11, AND MASSSPECTROMETRY.	17114649 [Abstract]
"A differential phosphoproteomic analysis of retinoic acid-treated P19cells."; Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.; J. Proteome Res. 6:3174-3186(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11; SER-12 AND TYR-17,AND MASS SPECTROMETRY.	17622165 [Abstract]
"Proteomic analysis of in vivo phosphorylated synaptic proteins."; Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I.,Blackstock W.P., Choudhary J.S., Grant S.G.; J. Biol. Chem. 280:5972-5982(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, AND MASSSPECTROMETRY.	15572359 [Abstract]

show

show