CSK_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CSK_RAT
• UniProt AC: P32577
• Protein Name: Tyrosine-protein kinase CSK
• Gene Name: Csk
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 450
• Subcellular Localization: Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts..
• Protein Description: Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK (By similarity)..
• Protein Sequence: MSAIQASWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCEGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTTDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYDVMKNCWHLDAATRPTFLQLREQLEHIRTHELHL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSAIQASWP ------CCCCCCCCC	33.79	-
2	Phosphorylation	------MSAIQASWP ------CCCCCCCCC	33.79	23984901
184	Phosphorylation	VAAQDEFYRSGWALN EECCHHHHHHCCCCC	11.21	21940666
263	Phosphorylation	VEEKGGLYIVTEYMA EEECCCEEEEEEEEC	9.16	28689409
273	Phosphorylation	TEYMAKGSLVDYLRS EEEECCCCHHHHHHH	25.23	28689409
277	Phosphorylation	AKGSLVDYLRSRGRS CCCCHHHHHHHCCCC	9.13	28689409
304	Phosphorylation	DVCEAMEYLEGNNFV HHHHHHHHHCCCCCC	9.29	-
364	Phosphorylation	ALREKKFSTKSDVWS HHHHCCCCCHHHHHH	43.75	-
416	Phosphorylation	DGCPPAVYDVMKNCW CCCCHHHHHHHHHCC	12.62	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
364	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
364	S	Phosphorylation	Phosphorylated at Ser-364 by PKA, leading to increased activity.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CSK_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PHAG1_RAT	Pag1	genetic	10801129
CXA1_RAT	Gja1	physical	27391443

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.