CDK6_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CDK6_MOUSE
• UniProt AC: Q64261
• Protein Name: Cyclin-dependent kinase 6
• Gene Name: Cdk6
• Organism: Mus musculus (Mouse).
• Sequence Length: 326
• Subcellular Localization: Cytoplasm . Nucleus . Cell projection, ruffle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus (By similarity). Present in the cytosol and
• Protein Description: Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans (By similarity). May play a role in the centrosome organization during the cell cycle phases..
• Protein Sequence: MEKDSLSRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTSEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDIIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYGALNHPYFQDLERYKDNLNSHLPSNQSTSELNTA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEKDSLSR -------CCCCHHCH	15.12	-
13	Phosphorylation	LSRADQQYECVAEIG HCHHHHHHHHEEEEC	12.98	-
24	Phosphorylation	AEIGEGAYGKVFKAR EEECCCCCCEEEEEE	28.47	25367039
49	Phosphorylation	LKRVRVQTSEEGMPL EEEEEEEECCCCCCH	34.94	-
50	Phosphorylation	KRVRVQTSEEGMPLS EEEEEEECCCCCCHH	19.29	25266776
70	Phosphorylation	AVLRHLETFEHPNVV HHHHHHHHCCCCCEE	40.46	-
83	Glutathionylation	VVRLFDVCTVSRTDR EEEEEEEEEEECCCC	3.15	24333276
177	Phosphorylation	YSFQMALTSVVVTLW HHHHHHHHHHHHHHH	15.22	-
222	Phosphorylation	RKPLFRGSSDVDQLG CCCCCCCCCCHHHHH	21.07	30635358
223	Phosphorylation	KPLFRGSSDVDQLGK CCCCCCCCCHHHHHH	44.47	30635358
264	Acetylation	KSAQPIEKFVTDIDE CCCCCHHHHCCCHHH	45.94	-
267	Phosphorylation	QPIEKFVTDIDELGK CCHHHHCCCHHHHCH	30.30	25195567
312	Phosphorylation	RYKDNLNSHLPSNQS HHHHHHHHCCCCCCC	29.57	23984901
316	Phosphorylation	NLNSHLPSNQSTSEL HHHHCCCCCCCHHHH	54.59	29472430
319	Phosphorylation	SHLPSNQSTSELNTA HCCCCCCCHHHHCCC	37.84	25521595
320	Phosphorylation	HLPSNQSTSELNTA- CCCCCCCHHHHCCC-	19.22	21743459
321	Phosphorylation	LPSNQSTSELNTA-- CCCCCCHHHHCCC--	45.38	25521595
325	Phosphorylation	QSTSELNTA------ CCHHHHCCC------	46.50	23984901

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of CDK6_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
177	T	Phosphorylation	Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CDK6_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CCND2_MOUSE	Ccnd2	physical	9584157
CCND3_MOUSE	Ccnd3	physical	11254678
RB_MOUSE	Rb1	physical	11254678
H11_MOUSE	Hist1h1a	physical	12007403

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.