CAV2_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CAV2_RAT
• UniProt AC: Q2IBC5
• Protein Name: Caveolin-2
• Gene Name: Cav2
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 162
• Subcellular Localization: Nucleus. Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr
• Protein Description: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression..
• Protein Sequence: MGLETEKADVQLFMADDAYSHHSVVDYTDPEKYVDSSQDRDPHQLNSHLKLGFEDLIAEPPTTHSFDKVWICSHALFEISKYVIYKFLTVFLAIPLAFIAGILFATLSCLHIWILMPFVKTCLMVLPSVQTIWKSVTDVVIGPLCTSVGRIFSSVSMQLSHD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
19	Phosphorylation	LFMADDAYSHHSVVD EEECCCCCCCCCCCC	18.61	21940666
20	Phosphorylation	FMADDAYSHHSVVDY EECCCCCCCCCCCCC	19.22	24945867
23	Phosphorylation	DDAYSHHSVVDYTDP CCCCCCCCCCCCCCH	20.57	25575281
27	Phosphorylation	SHHSVVDYTDPEKYV CCCCCCCCCCHHHHC	11.05	24945867
28	Phosphorylation	HHSVVDYTDPEKYVD CCCCCCCCCHHHHCC	40.38	25575281
36	Phosphorylation	DPEKYVDSSQDRDPH CHHHHCCCCCCCCHH	21.61	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
19	Y	Phosphorylation	Kinase	INSR	P15127	PSP
19	Y	Phosphorylation	Kinase	SRC	Q9WUD9	Uniprot
27	Y	Phosphorylation	Kinase	INSR	P15127	PSP
27	Y	Phosphorylation	Kinase	SRC	Q9WUD9	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
23	S	Phosphorylation	CAV1 promotes phosphorylation on Ser-23 which targets the complex to the plasma membrane, lipid rafts and caveolae.	19778377
36	S	Phosphorylation	Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity).	19778377
727	S	Phosphorylation	Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation.	19778377

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CAV2_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IRS1_HUMAN	IRS1	physical	25667086

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Caveolin-2 regulation of STAT3 transcriptional activation in responseto insulin.";
Kwon H., Jeong K., Hwang E.M., Park J.-Y., Hong S.-G., Choi W.-S.,Pak Y.;
Biochim. Biophys. Acta 1793:1325-1333(2009).
Cited for: PHOSPHORYLATION AT TYR-19 AND TYR-27, INTERACTION WITH MAPK1 ANDSTAT3, FUNCTION, SUBCELLLULAR LOCATION, AND MUTAGENESIS OF TYR-19 ANDTYR-27.
PubMed: 19427337

"Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis.";
Kwon H., Jeong K., Pak Y.;
J. Cell. Mol. Med. 13:1549-1564(2009).
Cited for: PHOSPHORYLATION AT TYR-19, INTERACTION WITH MAPK1 AND INSR, FUNCTION,SUBCELLLULAR LOCATION, AND MUTAGENESIS OF TYR-19.
PubMed: 19778377