dbPTM

BIN2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	BIN2_HUMAN
UniProt AC	Q9UBW5
Protein Name	Bridging integrator 2
Gene Name	BIN2
Organism	Homo sapiens (Human).
Sequence Length	565
Subcellular Localization	Cytoplasm . Cell projection, podosome membrane Peripheral membrane protein Cytoplasmic side . Cytoplasm, cell cortex . Cell projection, phagocytic cup . Associates with membranes enriched in phosphoinositides. Detected in the actin-rich cell cort
Protein Description	Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis..
Protein Sequence	MAEGKAGGAAGLFAKQVQKKFSRAQEKVLQKLGKAVETKDERFEQSASNFYQQQAEGHKLYKDLKNFLSAVKVMHESSKRVSETLQEIYSSEWDGHEELKAIVWNNDLLWEDYEEKLADQAVRTMEIYVAQFSEIKERIAKRGRKLVDYDSARHHLEAVQNAKKKDEAKTAKAEEEFNKAQTVFEDLNQELLEELPILYNSRIGCYVTIFQNISNLRDVFYREMSKLNHNLYEVMSKLEKQHSNKVFVVKGLSSSSRRSLVISPPVRTATVSSPLTSPTSPSTLSLKSESESVSATEDLAPDAAQGEDNSEIKELLEEEEIEKEGSEASSSEEDEPLPACNGPAQAQPSPTTERAKSQEEVLPSSTTPSPGGALSPSGQPSSSATEVVLRTRTASEGSEQPKKRASIQRTSAPPSRPPPPRATASPRPSSGNIPSSPTASGGGSPTSPRASLGTGTASPRTSLEVSPNPEPPEKPVRTPEAKENENIHNQNPEELCTSPTLMTSQVASEPGEAKKMEDKEKDNKLISANSSEGQDQLQVSMVPENNNLTAPEPQEEVSTSENPQL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Acetylation	---MAEGKAGGAAGL ---CCCCCHHHHHHH	35.05	19807561
5	Ubiquitination	---MAEGKAGGAAGL ---CCCCCHHHHHHH	35.05	-
19	Ubiquitination	LFAKQVQKKFSRAQE HHHHHHHHHHHHHHH	57.86	-
20	Acetylation	FAKQVQKKFSRAQEK HHHHHHHHHHHHHHH	31.33	19807569
27	Acetylation	KFSRAQEKVLQKLGK HHHHHHHHHHHHHHH	35.84	-
27	Ubiquitination	KFSRAQEKVLQKLGK HHHHHHHHHHHHHHH	35.84	24816145
33	Ubiquitination	EKVLQKLGKAVETKD HHHHHHHHHHHHHHH	23.94	29967540
36	Ubiquitination	LQKLGKAVETKDERF HHHHHHHHHHHHHHH	12.52	22817900
39	Ubiquitination	LGKAVETKDERFEQS HHHHHHHHHHHHHHH	44.05	-
39	Ubiquitination	LGKAVETKDERFEQS HHHHHHHHHHHHHHH	44.05	21890473
51	Phosphorylation	EQSASNFYQQQAEGH HHHHHHHHHHHHHHH	15.06	27642862
53	Ubiquitination	SASNFYQQQAEGHKL HHHHHHHHHHHHHHH	32.83	29967540
59	Acetylation	QQQAEGHKLYKDLKN HHHHHHHHHHHHHHH	65.45	-
59	Ubiquitination	QQQAEGHKLYKDLKN HHHHHHHHHHHHHHH	65.45	24816145
62	Ubiquitination	AEGHKLYKDLKNFLS HHHHHHHHHHHHHHH	68.00	22817900
65	Ubiquitination	HKLYKDLKNFLSAVK HHHHHHHHHHHHHHH	56.65	22817900
65 (in isoform 1)	Ubiquitination	-	56.65	21890473
65 (in isoform 2)	Ubiquitination	-	56.65	21890473
69	Phosphorylation	KDLKNFLSAVKVMHE HHHHHHHHHHHHHHH	27.21	-
77	Phosphorylation	AVKVMHESSKRVSET HHHHHHHHHHHHHHH	26.87	-
78	Phosphorylation	VKVMHESSKRVSETL HHHHHHHHHHHHHHH	22.84	-
89	Phosphorylation	SETLQEIYSSEWDGH HHHHHHHHHCCCCCH	12.85	28060719
90	Phosphorylation	ETLQEIYSSEWDGHE HHHHHHHHCCCCCHH	27.69	28060719
91	Phosphorylation	TLQEIYSSEWDGHEE HHHHHHHCCCCCHHH	26.24	28060719
91	Ubiquitination	TLQEIYSSEWDGHEE HHHHHHHCCCCCHHH	26.24	-
94	Ubiquitination	EIYSSEWDGHEELKA HHHHCCCCCHHHHHH	44.51	22817900
97	Ubiquitination	SSEWDGHEELKAIVW HCCCCCHHHHHHHHC	71.23	21890473
101	Phosphorylation	DGHEELKAIVWNNDL CCHHHHHHHHCCCCC	17.75	-
109	Phosphorylation	IVWNNDLLWEDYEEK HHCCCCCCCHHHHHH	5.40	-
110	Phosphorylation	VWNNDLLWEDYEEKL HCCCCCCCHHHHHHH	12.05	-
111	Ubiquitination	WNNDLLWEDYEEKLA CCCCCCCHHHHHHHH	51.16	-
113	Ubiquitination	NDLLWEDYEEKLADQ CCCCCHHHHHHHHHH	17.93	29967540
119	Ubiquitination	DYEEKLADQAVRTME HHHHHHHHHHHHHHH	47.62	-
124	Phosphorylation	LADQAVRTMEIYVAQ HHHHHHHHHHHHHHH	16.74	-
137	Ubiquitination	AQFSEIKERIAKRGR HHHHHHHHHHHHHCC	55.79	29967540
145	Ubiquitination	RIAKRGRKLVDYDSA HHHHHCCCCCCHHHH	56.54	-
149	Phosphorylation	RGRKLVDYDSARHHL HCCCCCCHHHHHHHH	12.34	-
151	Phosphorylation	RKLVDYDSARHHLEA CCCCCHHHHHHHHHH	22.45	-
164	Acetylation	EAVQNAKKKDEAKTA HHHHHHHHHHHHHHH	64.15	7467823
165	Acetylation	AVQNAKKKDEAKTAK HHHHHHHHHHHHHHH	61.16	7467833
169	Acetylation	AKKKDEAKTAKAEEE HHHHHHHHHHHHHHH	47.14	7467843
177	Ubiquitination	TAKAEEEFNKAQTVF HHHHHHHHHHHHHHH	14.91	-
183	Phosphorylation	EFNKAQTVFEDLNQE HHHHHHHHHHHHHHH	3.08	-
195	Ubiquitination	NQELLEELPILYNSR HHHHHHHCCHHHHCC	2.03	-
211	Ubiquitination	GCYVTIFQNISNLRD CEEEEEECCHHHHHH	42.36	29967540
214	Phosphorylation	VTIFQNISNLRDVFY EEEECCHHHHHHHHH	37.04	24719451
224	Ubiquitination	RDVFYREMSKLNHNL HHHHHHHHHHHCCHH	2.95	-
225	Phosphorylation	DVFYREMSKLNHNLY HHHHHHHHHHCCHHH	29.28	24719451
232	Phosphorylation	SKLNHNLYEVMSKLE HHHCCHHHHHHHHHH	16.28	28270605
236	Phosphorylation	HNLYEVMSKLEKQHS CHHHHHHHHHHHHHC	38.98	24719451
243	Phosphorylation	SKLEKQHSNKVFVVK HHHHHHHCCCEEEEE	35.53	21949786
250	Ubiquitination	SNKVFVVKGLSSSSR CCCEEEEEECCCCCC	49.16	-
257	Phosphorylation	KGLSSSSRRSLVISP EECCCCCCCEEEECC	33.87	-
259	Phosphorylation	LSSSSRRSLVISPPV CCCCCCCEEEECCCC	26.36	23401153
263	Phosphorylation	SRRSLVISPPVRTAT CCCEEEECCCCCEEE	18.70	22115753
264	Phosphorylation	RRSLVISPPVRTATV CCEEEECCCCCEEEE	21.93	-
268	Phosphorylation	VISPPVRTATVSSPL EECCCCCEEEECCCC	27.24	22115753
269	Ubiquitination	ISPPVRTATVSSPLT ECCCCCEEEECCCCC	8.74	-
270	Phosphorylation	SPPVRTATVSSPLTS CCCCCEEEECCCCCC	21.95	22115753
272	Phosphorylation	PVRTATVSSPLTSPT CCCEEEECCCCCCCC	23.14	23401153
273	Phosphorylation	VRTATVSSPLTSPTS CCEEEECCCCCCCCC	21.42	23401153
276	Phosphorylation	ATVSSPLTSPTSPST EEECCCCCCCCCCCC	35.72	23401153
277	Phosphorylation	TVSSPLTSPTSPSTL EECCCCCCCCCCCCE	33.59	23911959
279	Phosphorylation	SSPLTSPTSPSTLSL CCCCCCCCCCCCEEC	53.70	22115753
280	Phosphorylation	SPLTSPTSPSTLSLK CCCCCCCCCCCEECC	21.90	22115753
282	Phosphorylation	LTSPTSPSTLSLKSE CCCCCCCCCEECCCC	41.57	22115753
282	Ubiquitination	LTSPTSPSTLSLKSE CCCCCCCCCEECCCC	41.57	-
283	Phosphorylation	TSPTSPSTLSLKSES CCCCCCCCEECCCCC	24.61	30108239
285	Phosphorylation	PTSPSTLSLKSESES CCCCCCEECCCCCCC	33.56	22499768
288	Phosphorylation	PSTLSLKSESESVSA CCCEECCCCCCCCCC	52.20	28060719
290	Phosphorylation	TLSLKSESESVSATE CEECCCCCCCCCCCC	42.11	28060719
291	Phosphorylation	LSLKSESESVSATED EECCCCCCCCCCCCC	51.62	27251275
292	Phosphorylation	SLKSESESVSATEDL ECCCCCCCCCCCCCC	30.56	29970186
294	Phosphorylation	KSESESVSATEDLAP CCCCCCCCCCCCCCC	38.46	28060719
295	Phosphorylation	SESESVSATEDLAPD CCCCCCCCCCCCCCC	16.92	27251275
296	Phosphorylation	ESESVSATEDLAPDA CCCCCCCCCCCCCCH	23.64	28060719
304	Phosphorylation	EDLAPDAAQGEDNSE CCCCCCHHCCCCCHH	25.87	27251275
305	Phosphorylation	DLAPDAAQGEDNSEI CCCCCHHCCCCCHHH	58.49	-
308	Phosphorylation	PDAAQGEDNSEIKEL CCHHCCCCCHHHHHH	71.43	27251275
309	Phosphorylation	DAAQGEDNSEIKELL CHHCCCCCHHHHHHH	36.92	-
310	Phosphorylation	AAQGEDNSEIKELLE HHCCCCCHHHHHHHH	53.68	28270605
311	Phosphorylation	AQGEDNSEIKELLEE HCCCCCHHHHHHHHH	65.42	27251275
312	Phosphorylation	QGEDNSEIKELLEEE CCCCCHHHHHHHHHH	4.16	27251275
317	Phosphorylation	SEIKELLEEEEIEKE HHHHHHHHHHHHHHC	75.81	27251275
326	Phosphorylation	EEIEKEGSEASSSEE HHHHHCCCCCCCCCC	30.95	28270605
329	Phosphorylation	EKEGSEASSSEEDEP HHCCCCCCCCCCCCC	30.28	28270605
330	Phosphorylation	KEGSEASSSEEDEPL HCCCCCCCCCCCCCC	49.14	28270605
331	Phosphorylation	EGSEASSSEEDEPLP CCCCCCCCCCCCCCC	42.37	28270605
342	Phosphorylation	EPLPACNGPAQAQPS CCCCCCCCCCCCCCC	20.85	27251275
349	Phosphorylation	GPAQAQPSPTTERAK CCCCCCCCCCCHHHC	23.85	29970186
350	Ubiquitination	PAQAQPSPTTERAKS CCCCCCCCCCHHHCH	50.45	24816145
351	Phosphorylation	AQAQPSPTTERAKSQ CCCCCCCCCHHHCHH	45.70	28270605
352	Phosphorylation	QAQPSPTTERAKSQE CCCCCCCCHHHCHHH	27.19	20058876
357	Phosphorylation	PTTERAKSQEEVLPS CCCHHHCHHHHHCCC	41.67	23401153
364	Phosphorylation	SQEEVLPSSTTPSPG HHHHHCCCCCCCCCC	35.83	28450419
365	Phosphorylation	QEEVLPSSTTPSPGG HHHHCCCCCCCCCCC	34.07	28450419
366	Phosphorylation	EEVLPSSTTPSPGGA HHHCCCCCCCCCCCC	47.25	25693802
367	Phosphorylation	EVLPSSTTPSPGGAL HHCCCCCCCCCCCCC	24.58	25693802
369	Phosphorylation	LPSSTTPSPGGALSP CCCCCCCCCCCCCCC	33.80	25693802
375	Phosphorylation	PSPGGALSPSGQPSS CCCCCCCCCCCCCCC	19.24	25693802
377	Phosphorylation	PGGALSPSGQPSSSA CCCCCCCCCCCCCCC	46.84	25693802
381	Phosphorylation	LSPSGQPSSSATEVV CCCCCCCCCCCEEEE	29.10	28450419
382	Phosphorylation	SPSGQPSSSATEVVL CCCCCCCCCCEEEEE	30.58	28450419
383	Phosphorylation	PSGQPSSSATEVVLR CCCCCCCCCEEEEEE	43.47	28450419
384	Phosphorylation	SGQPSSSATEVVLRT CCCCCCCCEEEEEEE	15.10	20058876
385	Phosphorylation	GQPSSSATEVVLRTR CCCCCCCEEEEEEEE	31.12	29978859
389	Phosphorylation	SSATEVVLRTRTASE CCCEEEEEEEECCCC	5.82	27251275
391	Phosphorylation	ATEVVLRTRTASEGS CEEEEEEEECCCCCC	28.36	28270605
393	Phosphorylation	EVVLRTRTASEGSEQ EEEEEEECCCCCCCC	33.64	28450419
395	Phosphorylation	VLRTRTASEGSEQPK EEEEECCCCCCCCCC	41.71	22115753
398	Phosphorylation	TRTASEGSEQPKKRA EECCCCCCCCCCCCC	29.07	28450419
401	Phosphorylation	ASEGSEQPKKRASIQ CCCCCCCCCCCCCCC	39.83	27251275
406	Phosphorylation	EQPKKRASIQRTSAP CCCCCCCCCCCCCCC	24.24	26074081
410	Phosphorylation	KRASIQRTSAPPSRP CCCCCCCCCCCCCCC	16.92	24043423
411	Phosphorylation	RASIQRTSAPPSRPP CCCCCCCCCCCCCCC	40.75	22617229
415	Phosphorylation	QRTSAPPSRPPPPRA CCCCCCCCCCCCCCC	57.25	26074081
423	Phosphorylation	RPPPPRATASPRPSS CCCCCCCCCCCCCCC	29.02	23911959
425	Phosphorylation	PPPRATASPRPSSGN CCCCCCCCCCCCCCC	19.65	23401153
427	Phosphorylation	PRATASPRPSSGNIP CCCCCCCCCCCCCCC	41.03	27251275
429	Phosphorylation	ATASPRPSSGNIPSS CCCCCCCCCCCCCCC	52.75	23911959
430	Phosphorylation	TASPRPSSGNIPSSP CCCCCCCCCCCCCCC	38.24	23401153
435	Phosphorylation	PSSGNIPSSPTASGG CCCCCCCCCCCCCCC	45.19	23401153
436	Phosphorylation	SSGNIPSSPTASGGG CCCCCCCCCCCCCCC	22.40	23401153
438	Phosphorylation	GNIPSSPTASGGGSP CCCCCCCCCCCCCCC	35.09	23401153
440	Phosphorylation	IPSSPTASGGGSPTS CCCCCCCCCCCCCCC	40.21	20058876
442	Phosphorylation	SSPTASGGGSPTSPR CCCCCCCCCCCCCCC	30.80	27251275
443	Phosphorylation	SPTASGGGSPTSPRA CCCCCCCCCCCCCCC	33.70	27251275
444	Phosphorylation	PTASGGGSPTSPRAS CCCCCCCCCCCCCCC	28.42	23401153
446	Phosphorylation	ASGGGSPTSPRASLG CCCCCCCCCCCCCCC	53.73	23401153
447	Phosphorylation	SGGGSPTSPRASLGT CCCCCCCCCCCCCCC	18.77	23401153
448	Ubiquitination	GGGSPTSPRASLGTG CCCCCCCCCCCCCCC	38.03	24816145
451	Phosphorylation	SPTSPRASLGTGTAS CCCCCCCCCCCCCCC	28.72	20058876
454	Phosphorylation	SPRASLGTGTASPRT CCCCCCCCCCCCCCC	36.33	27690223
455	Phosphorylation	PRASLGTGTASPRTS CCCCCCCCCCCCCCC	19.93	27251275
456	Phosphorylation	RASLGTGTASPRTSL CCCCCCCCCCCCCCC	24.65	23401153
457	Phosphorylation	ASLGTGTASPRTSLE CCCCCCCCCCCCCCC	20.91	-
458	Phosphorylation	SLGTGTASPRTSLEV CCCCCCCCCCCCCCC	18.11	23401153
461	Phosphorylation	TGTASPRTSLEVSPN CCCCCCCCCCCCCCC	40.84	23401153
462	Phosphorylation	GTASPRTSLEVSPNP CCCCCCCCCCCCCCC	24.66	20058876
466	Phosphorylation	PRTSLEVSPNPEPPE CCCCCCCCCCCCCCC	15.02	23401153
467	Phosphorylation	RTSLEVSPNPEPPEK CCCCCCCCCCCCCCC	66.62	-
468	Phosphorylation	TSLEVSPNPEPPEKP CCCCCCCCCCCCCCC	46.17	20058876
470	Phosphorylation	LEVSPNPEPPEKPVR CCCCCCCCCCCCCCC	78.94	20058876
472	Phosphorylation	VSPNPEPPEKPVRTP CCCCCCCCCCCCCCC	61.66	20058876
473	Ubiquitination	SPNPEPPEKPVRTPE CCCCCCCCCCCCCCC	79.35	24816145
474	Ubiquitination	PNPEPPEKPVRTPEA CCCCCCCCCCCCCCH	55.37	24816145
476	Phosphorylation	PEPPEKPVRTPEAKE CCCCCCCCCCCCHHH	18.44	20058876
478	Phosphorylation	PPEKPVRTPEAKENE CCCCCCCCCCHHHCC	26.22	23401153
479	Phosphorylation	PEKPVRTPEAKENEN CCCCCCCCCHHHCCC	29.05	27251275
483	Phosphorylation	VRTPEAKENENIHNQ CCCCCHHHCCCCCCC	76.46	20058876
486	Phosphorylation	PEAKENENIHNQNPE CCHHHCCCCCCCCHH	53.02	20166139
488	Phosphorylation	AKENENIHNQNPEEL HHHCCCCCCCCHHHH	41.21	20058876
490	Phosphorylation	ENENIHNQNPEELCT HCCCCCCCCHHHHCC	53.33	20058876
493	Phosphorylation	NIHNQNPEELCTSPT CCCCCCHHHHCCCCC	71.76	20058876
494	Phosphorylation	IHNQNPEELCTSPTL CCCCCHHHHCCCCCC	50.86	20058876
497	Phosphorylation	QNPEELCTSPTLMTS CCHHHHCCCCCCCCC	50.82	23401153
498	Phosphorylation	NPEELCTSPTLMTSQ CHHHHCCCCCCCCCC	18.19	23401153
500	Phosphorylation	EELCTSPTLMTSQVA HHHCCCCCCCCCCCC	29.00	28464451
503	Phosphorylation	CTSPTLMTSQVASEP CCCCCCCCCCCCCCC	21.08	30108239
504	Phosphorylation	TSPTLMTSQVASEPG CCCCCCCCCCCCCCC	14.53	30108239
506	Ubiquitination	PTLMTSQVASEPGEA CCCCCCCCCCCCCCC	6.74	24816145
508	Phosphorylation	LMTSQVASEPGEAKK CCCCCCCCCCCCCCC	45.53	28450419
510	Phosphorylation	TSQVASEPGEAKKME CCCCCCCCCCCCCCC	43.35	27251275
530	Phosphorylation	NKLISANSSEGQDQL CCEECCCCCCCCCCE	29.50	27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of BIN2_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of BIN2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of BIN2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
BIN1_HUMAN	BIN1	physical	28514442
AMPH_HUMAN	AMPH	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of BIN2_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259; SER-273; SER-357;SER-436; THR-438; SER-444; SER-447 AND SER-458, AND MASS SPECTROMETRY.	19690332 [Abstract]
"Phosphorylation analysis of primary human T lymphocytes usingsequential IMAC and titanium oxide enrichment."; Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.; J. Proteome Res. 7:5167-5176(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, AND MASSSPECTROMETRY.	19367720 [Abstract]