BIG3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BIG3_HUMAN
• UniProt AC: Q5TH69
• Protein Name: Brefeldin A-inhibited guanine nucleotide-exchange protein 3 {ECO:0000305}
• Gene Name: ARFGEF3 {ECO:0000312|HGNC:HGNC:21213}
• Organism: Homo sapiens (Human).
• Sequence Length: 2177
• Subcellular Localization: Cytoplasm . Cytoplasmic vesicle, secretory vesicle . Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein .
• Protein Description: Participates in the regulation of systemic glucose homeostasis, where it negatively regulates insulin granule biogenesis in pancreatic islet beta cells (By similarity). Also regulates glucagon granule production in pancreatic alpha cells (By similarity). Inhibits nuclear translocation of the transcriptional coregulator PHB2 and may enhance estrogen receptor alpha (ESR1) transcriptional activity in breast cancer cells. [PubMed: 19496786]
• Protein Sequence: MEEILRKLQKEASGSKYKAIKESCTWALETLGGLDTIVKIPPHVLREKCLLPLQLALESKNVKLAQHALAGMQKLLSEERFVSMETDSDEKQLLNQILNAVKVTPSLNEDLQVEVMKVLLCITYTPTFDLNGSAVLKIAEVCIETYISSCHQRSINTAVRATLSQMLSDLTLQLRQRQENTIIENPDVPQDFGNQGSTVESLCDDVVSVLTVLCEKLQAAINDSQQLQLLYLECILSVLSSSSSSMHLHRRFTDLIWKNLCPALIVILGNPIHDKTITSAHTSSTSTSLESDSASPGVSDHGRGSGCSCTAPALSGPVARTIYYIAAELVRLVGSVDSMKPVLQSLYHRVLLYPPPQHRVEAIKIMKEILGSPQRLCDLAGPSSTESESRKRSISKRKSHLDLLKLIMDGMTEACIKGGIEACYAAVSCVCTLLGALDELSQGKGLSEGQVQLLLLRLEELKDGAEWSRDSMEINEADFRWQRRVLSSEHTPWESGNERSLDISISVTTDTGQTTLEGELGQTTPEDHSGNHKNSLKSPAIPEGKETLSKVLETEAVDQPDVVQRSHTVPYPDITNFLSVDCRTRSYGSRYSESNFSVDDQDLSRTEFDSCDQYSMAAEKDSGRSDVSDIGSDNCSLADEEQTPRDCLGHRSLRTAALSLKLLKNQEADQHSARLFIQSLEGLLPRLLSLSNVEEVDTALQNFASTFCSGMMHSPGFDGNSSLSFQMLMNADSLYTAAHCALLLNLKLSHGDYYRKRPTLAPGVMKDFMKQVQTSGVLMVFSQAWIEELYHQVLDRNMLGEAGYWGSPEDNSLPLITMLTDIDGLESSAIGGQLMASAATESPFAQSRRIDDSTVAGVAFARYILVGCWKNLIDTLSTPLTGRMAGSSKGLAFILGAEGIKEQNQKERDAICMSLDGLRKAARLSCALGVAANCASALAQMAAASCVQEEKEEREAQEPSDAITQVKLKVEQKLEQIGKVQGVWLHTAHVLCMEAILSVGLEMGSHNPDCWPHVFRVCEYVGTLEHNHFSDGASQPPLTISQPQKATGSAGLLGDPECEGSPPEHSPEQGRSLSTAPVVQPLSIQDLVREGSRGRASDFRGGSLMSGSSAAKVVLTLSTQADRLFEDATDKLNLMALGGFLYQLKKASQSQLFHSVTDTVDYSLAMPGEVKSTQDRKSALHLFRLGNAMLRIVRSKARPLLHVMRCWSLVAPHLVEAACHKERHVSQKAVSFIHDILTEVLTDWNEPPHFHFNEALFRPFERIMQLELCDEDVQDQVVTSIGELVEVCSTQIQSGWRPLFSALETVHGGNKSEMKEYLVGDYSMGKGQAPVFDVFEAFLNTDNIQVFANAATSYIMCLMKFVKGLGEVDCKEIGDCAPAPGAPSTDLCLPALDYLRRCSQLLAKIYKMPLKPIFLSGRLAGLPRRLQEQSASSEDGIESVLSDFDDDTGLIEVWIILLEQLTAAVSNCPRQHQPPTLDLLFELLRDVTKTPGPGFGIYAVVHLLLPVMSVWLRRSHKDHSYWDMASANFKHAIGLSCELVVEHIQSFLHSDIRYESMINTMLKDLFELLVACVAKPTETISRVGCSCIRYVLVTAGPVFTEEMWRLACCALQDAFSATLKPVKDLLGCFHSGTESFSGEGCQVRVAAPSSSPSAEAEYWRIRAMAQQVFMLDTQCSPKTPNNFDHAQSCQLIIELPPDEKPNGHTKKSVSFREIVVSLLSHQVLLQNLYDILLEEFVKGPSPGEEKTIQVPEAKLAGFLRYISMQNLAVIFDLLLDSYRTAREFDTSPGLKCLLKKVSGIGGAANLYRQSAMSFNIYFHALVCAVLTNQETITAEQVKKVLFEDDERSTDSSQQCSSEDEDIFEETAQVSPPRGKEKRQWRARMPLLSVQPVSNADWVWLVKRLHKLCMELCNNYIQMHLDLENCMEEPPIFKGDPFFILPSFQSESSTPSTGGFSGKETPSEDDRSQSREHMGESLSLKAGGGDLLLPPSPKVEKKDPSRKKEWWENAGNKIYTMAADKTISKLMTEYKKRKQQHNLSAFPKEVKVEKKGEPLGPRGQDSPLLQRPQHLMDQGQMRHSFSAGPELLRQDKRPRSGSTGSSLSVSVRDAEAQIQAWTNMVLTVLNQIQILPDQTFTALQPAVFPCISQLTCHVTDIRVRQAVREWLGRVGRVYDIIV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
15	Phosphorylation	LQKEASGSKYKAIKE HHHHHCCHHHHHHHH	30.25	-
60	Ubiquitination	LQLALESKNVKLAQH HHHHHHCCCHHHHHH	57.28	30230243
83	Phosphorylation	LSEERFVSMETDSDE HCCCCCCCCCCCCHH	14.27	28985074
86	Phosphorylation	ERFVSMETDSDEKQL CCCCCCCCCCHHHHH	31.96	-
124	Phosphorylation	KVLLCITYTPTFDLN HHHHHHEECCCCCCC	7.27	-
224	Phosphorylation	LQAAINDSQQLQLLY HHHHCCHHHHHHHHH	18.48	24043423
231	Phosphorylation	SQQLQLLYLECILSV HHHHHHHHHHHHHHH	14.73	24043423
237	Phosphorylation	LYLECILSVLSSSSS HHHHHHHHHHCCCCC	10.91	24043423
240	Phosphorylation	ECILSVLSSSSSSMH HHHHHHHCCCCCCCH	26.47	24043423
241	Phosphorylation	CILSVLSSSSSSMHL HHHHHHCCCCCCCHH	30.03	24043423
242	Phosphorylation	ILSVLSSSSSSMHLH HHHHHCCCCCCCHHH	30.75	24043423
243	Phosphorylation	LSVLSSSSSSMHLHR HHHHCCCCCCCHHHH	28.51	24043423
244	Phosphorylation	SVLSSSSSSMHLHRR HHHCCCCCCCHHHHH	33.36	24043423
245	Phosphorylation	VLSSSSSSMHLHRRF HHCCCCCCCHHHHHH	16.51	24043423
283	Phosphorylation	TITSAHTSSTSTSLE CCCCCCCCCCCCCCC	23.04	23312004
284	Phosphorylation	ITSAHTSSTSTSLES CCCCCCCCCCCCCCC	27.77	23312004
285	Phosphorylation	TSAHTSSTSTSLESD CCCCCCCCCCCCCCC	35.47	23312004
286	Phosphorylation	SAHTSSTSTSLESDS CCCCCCCCCCCCCCC	20.25	23312004
287	Phosphorylation	AHTSSTSTSLESDSA CCCCCCCCCCCCCCC	37.10	23312004
288	Phosphorylation	HTSSTSTSLESDSAS CCCCCCCCCCCCCCC	29.88	23312004
291	Phosphorylation	STSTSLESDSASPGV CCCCCCCCCCCCCCC	42.00	28348404
293	Phosphorylation	STSLESDSASPGVSD CCCCCCCCCCCCCCC	39.67	28348404
295	Phosphorylation	SLESDSASPGVSDHG CCCCCCCCCCCCCCC	26.58	28348404
299	Phosphorylation	DSASPGVSDHGRGSG CCCCCCCCCCCCCCC	29.79	23312004
372	Phosphorylation	IMKEILGSPQRLCDL HHHHHHCCHHHHHHH	17.89	22210691
383	Phosphorylation	LCDLAGPSSTESESR HHHHCCCCCCCCHHH	49.31	27251275
384	Phosphorylation	CDLAGPSSTESESRK HHHCCCCCCCCHHHH	39.05	27251275
385	Phosphorylation	DLAGPSSTESESRKR HHCCCCCCCCHHHHH	48.11	22210691
387	Phosphorylation	AGPSSTESESRKRSI CCCCCCCCHHHHHCH	40.75	22210691
389	Phosphorylation	PSSTESESRKRSISK CCCCCCHHHHHCHHH	53.00	27282143
395	Phosphorylation	ESRKRSISKRKSHLD HHHHHCHHHHHHHHH	27.88	27282143
399	Phosphorylation	RSISKRKSHLDLLKL HCHHHHHHHHHHHHH	33.06	24719451
468	Phosphorylation	LKDGAEWSRDSMEIN HCCCCCCCHHHCCCC	20.09	28102081
471	Phosphorylation	GAEWSRDSMEINEAD CCCCCHHHCCCCHHH	19.73	27422710
487	Phosphorylation	RWQRRVLSSEHTPWE HHHHHHHCCCCCCCC	30.72	28348404
488	Phosphorylation	WQRRVLSSEHTPWES HHHHHHCCCCCCCCC	29.27	28348404
491	Phosphorylation	RVLSSEHTPWESGNE HHHCCCCCCCCCCCC	26.50	28348404
495	Phosphorylation	SEHTPWESGNERSLD CCCCCCCCCCCCEEE	42.95	28348404
535	Phosphorylation	HSGNHKNSLKSPAIP CCCCCCCCCCCCCCC	41.81	23312004
538	Phosphorylation	NHKNSLKSPAIPEGK CCCCCCCCCCCCCCH	25.51	25849741
589	Phosphorylation	CRTRSYGSRYSESNF CCCCCCCCCCCCCCC	21.58	24719451
592	Phosphorylation	RSYGSRYSESNFSVD CCCCCCCCCCCCCCC	33.18	25849741
594	Phosphorylation	YGSRYSESNFSVDDQ CCCCCCCCCCCCCHH	36.92	30243723
597	Phosphorylation	RYSESNFSVDDQDLS CCCCCCCCCCHHHCC	28.96	19664994
610	Phosphorylation	LSRTEFDSCDQYSMA CCCCCCCCCHHHHHH	25.55	27251275
614	Phosphorylation	EFDSCDQYSMAAEKD CCCCCHHHHHHHHCC	6.27	27642862
622	Phosphorylation	SMAAEKDSGRSDVSD HHHHHCCCCCCCHHH	47.36	23312004
625	Phosphorylation	AEKDSGRSDVSDIGS HHCCCCCCCHHHCCC	45.98	25849741
628	Phosphorylation	DSGRSDVSDIGSDNC CCCCCCHHHCCCCCC	28.62	25849741
632	Phosphorylation	SDVSDIGSDNCSLAD CCHHHCCCCCCCCCC	26.45	30278072
636	Phosphorylation	DIGSDNCSLADEEQT HCCCCCCCCCCCCCC	32.61	30278072
643	Phosphorylation	SLADEEQTPRDCLGH CCCCCCCCHHHHCCH	24.46	27251275
655	Phosphorylation	LGHRSLRTAALSLKL CCHHHHHHHHHHHHH	22.90	-
659	Phosphorylation	SLRTAALSLKLLKNQ HHHHHHHHHHHHHCC	20.80	24719451
887	Phosphorylation	LTGRMAGSSKGLAFI CCCCCCCCCHHHHHH	21.24	22210691
888	Phosphorylation	TGRMAGSSKGLAFIL CCCCCCCCHHHHHHH	29.80	22210691
1047	Phosphorylation	ISQPQKATGSAGLLG ECCCCCCCCCCCCCC	38.41	29691806
1049	Phosphorylation	QPQKATGSAGLLGDP CCCCCCCCCCCCCCC	18.14	26657352
1061	Phosphorylation	GDPECEGSPPEHSPE CCCCCCCCCCCCCHH	18.26	30278072
1066	Phosphorylation	EGSPPEHSPEQGRSL CCCCCCCCHHCCCCC	28.59	30278072
1072	Phosphorylation	HSPEQGRSLSTAPVV CCHHCCCCCCCCCCC	33.23	24719451
1074	Phosphorylation	PEQGRSLSTAPVVQP HHCCCCCCCCCCCCC	24.62	28348404
1075	Phosphorylation	EQGRSLSTAPVVQPL HCCCCCCCCCCCCCC	40.03	28348404
1083	Phosphorylation	APVVQPLSIQDLVRE CCCCCCCCHHHHHHC	25.53	28348404
1092	Phosphorylation	QDLVREGSRGRASDF HHHHHCCCCCCCCCC	27.15	27134283
1093	Methylation	DLVREGSRGRASDFR HHHHCCCCCCCCCCC	49.85	-
1097	Phosphorylation	EGSRGRASDFRGGSL CCCCCCCCCCCCCCC	35.60	27134283
1103	Phosphorylation	ASDFRGGSLMSGSSA CCCCCCCCCCCCCCC	24.59	27251275
1109	Phosphorylation	GSLMSGSSAAKVVLT CCCCCCCCCHHHHHE	35.45	27134283
1116	Phosphorylation	SAAKVVLTLSTQADR CCHHHHHEEHHHHHH	13.67	23403867
1119	Phosphorylation	KVVLTLSTQADRLFE HHHHEEHHHHHHHCC	30.78	-
1150	Phosphorylation	QLKKASQSQLFHSVT HHHHHCHHHHCCCCC	26.76	30576142
1155	Phosphorylation	SQSQLFHSVTDTVDY CHHHHCCCCCCCCCH	21.27	30576142
1162	Phosphorylation	SVTDTVDYSLAMPGE CCCCCCCHHHCCCCC	10.97	27642862
1178	Phosphorylation	KSTQDRKSALHLFRL CCCCCHHHHHHHHHH	36.64	-
1322	Phosphorylation	KEYLVGDYSMGKGQA HHHHCCCCCCCCCCC	8.47	27642862
1323	Phosphorylation	EYLVGDYSMGKGQAP HHHCCCCCCCCCCCC	26.29	27251275
1406	Phosphorylation	SQLLAKIYKMPLKPI HHHHHHHHCCCCCCH	10.67	22817900
1416	Phosphorylation	PLKPIFLSGRLAGLP CCCCHHCCCCCCCCC	15.89	24719451
1631	Phosphorylation	DLLGCFHSGTESFSG HHHCCCCCCCCCCCC	27.45	23312004
1633	Phosphorylation	LGCFHSGTESFSGEG HCCCCCCCCCCCCCC	31.32	23312004
1635	Phosphorylation	CFHSGTESFSGEGCQ CCCCCCCCCCCCCCE	25.26	23312004
1637	Phosphorylation	HSGTESFSGEGCQVR CCCCCCCCCCCCEEE	45.26	23312004
1649	Phosphorylation	QVRVAAPSSSPSAEA EEEEECCCCCCCHHH	38.43	29116813
1650	Phosphorylation	VRVAAPSSSPSAEAE EEEECCCCCCCHHHH	45.25	21815630
1651	Phosphorylation	RVAAPSSSPSAEAEY EEECCCCCCCHHHHH	27.49	25159151
1653	Phosphorylation	AAPSSSPSAEAEYWR ECCCCCCCHHHHHHH	40.46	27251275
1673	Phosphorylation	QQVFMLDTQCSPKTP HHHHHCCCCCCCCCC	27.75	24719451
1676	Phosphorylation	FMLDTQCSPKTPNNF HHCCCCCCCCCCCCC	21.44	24076635
1710	Phosphorylation	GHTKKSVSFREIVVS CCCCCCCCHHHHHHH	26.19	24719451
1741	Phosphorylation	EEFVKGPSPGEEKTI HHHHCCCCCCCCCCC	54.96	28985074
1747	Phosphorylation	PSPGEEKTIQVPEAK CCCCCCCCCCCCHHH	21.66	-
1761	Phosphorylation	KLAGFLRYISMQNLA HHHHHHHHHHHHHHH	10.25	-
1763	Phosphorylation	AGFLRYISMQNLAVI HHHHHHHHHHHHHHH	12.84	-
1787	Phosphorylation	TAREFDTSPGLKCLL HHHHCCCCHHHHHHH	20.86	24719451
1798	Phosphorylation	KCLLKKVSGIGGAAN HHHHHHHCCCCHHHH	33.31	22210691
1807	Phosphorylation	IGGAANLYRQSAMSF CCHHHHHHHHHHHHH	13.35	22210691
1848	Phosphorylation	LFEDDERSTDSSQQC HHCCCCCCCCCCCCC	34.22	27251275
1849	Phosphorylation	FEDDERSTDSSQQCS HCCCCCCCCCCCCCC	46.23	26330541
1851	Phosphorylation	DDERSTDSSQQCSSE CCCCCCCCCCCCCCC	30.09	26330541
1852	Phosphorylation	DERSTDSSQQCSSED CCCCCCCCCCCCCCC	27.38	26330541
1856	Phosphorylation	TDSSQQCSSEDEDIF CCCCCCCCCCCHHHH	32.21	29802988
1857	Phosphorylation	DSSQQCSSEDEDIFE CCCCCCCCCCHHHHH	57.61	30576142
1866	Phosphorylation	DEDIFEETAQVSPPR CHHHHHHHHCCCCCC	18.39	26330541
1870	Phosphorylation	FEETAQVSPPRGKEK HHHHHCCCCCCCHHH	19.67	26330541
1888	Phosphorylation	RARMPLLSVQPVSNA HHCCCCEECCCCCCC	26.79	28857561
1942	Phosphorylation	DPFFILPSFQSESST CCEEEECCCCCCCCC	32.26	27251275
1945	Phosphorylation	FILPSFQSESSTPST EEECCCCCCCCCCCC	37.12	27251275
1947	Phosphorylation	LPSFQSESSTPSTGG ECCCCCCCCCCCCCC	44.77	27251275
1948	Phosphorylation	PSFQSESSTPSTGGF CCCCCCCCCCCCCCC	40.04	27251275
1949	Phosphorylation	SFQSESSTPSTGGFS CCCCCCCCCCCCCCC	30.45	27251275
1951	Phosphorylation	QSESSTPSTGGFSGK CCCCCCCCCCCCCCC	39.51	27251275
1956	Phosphorylation	TPSTGGFSGKETPSE CCCCCCCCCCCCCCC	52.83	28348404
1960	Phosphorylation	GGFSGKETPSEDDRS CCCCCCCCCCCCCCH	35.28	28348404
1962	Phosphorylation	FSGKETPSEDDRSQS CCCCCCCCCCCCHHH	62.16	28348404
1967	Phosphorylation	TPSEDDRSQSREHMG CCCCCCCHHHHHHHH	39.23	28348404
1969	Phosphorylation	SEDDRSQSREHMGES CCCCCHHHHHHHHCC	40.66	28348404
1976	Phosphorylation	SREHMGESLSLKAGG HHHHHHCCCCEECCC	19.96	25849741
1978	Phosphorylation	EHMGESLSLKAGGGD HHHHCCCCEECCCCC	36.78	29523821
1991	Phosphorylation	GDLLLPPSPKVEKKD CCCCCCCCCCCCCCC	35.10	19664994
2014	Phosphorylation	ENAGNKIYTMAADKT HHCCCHHHHHHHHHH	7.65	29759185
2021	Phosphorylation	YTMAADKTISKLMTE HHHHHHHHHHHHHHH	30.91	29759185
2023	Phosphorylation	MAADKTISKLMTEYK HHHHHHHHHHHHHHH	25.60	-
2029	Phosphorylation	ISKLMTEYKKRKQQH HHHHHHHHHHHHHHC	16.79	-
2061	Phosphorylation	LGPRGQDSPLLQRPQ CCCCCCCCCCHHCCH	15.01	22617229
2079	Phosphorylation	DQGQMRHSFSAGPEL HHCCCCCCCCCCHHH	15.55	25849741
2081	Phosphorylation	GQMRHSFSAGPELLR CCCCCCCCCCHHHHH	35.02	29691806
2095	Phosphorylation	RQDKRPRSGSTGSSL HCCCCCCCCCCCCCE	39.52	30278072
2097	Phosphorylation	DKRPRSGSTGSSLSV CCCCCCCCCCCCEEE	30.73	21955146
2098	Phosphorylation	KRPRSGSTGSSLSVS CCCCCCCCCCCEEEE	44.61	30278072
2100	Phosphorylation	PRSGSTGSSLSVSVR CCCCCCCCCEEEEHH	28.35	30278072
2101	Phosphorylation	RSGSTGSSLSVSVRD CCCCCCCCEEEEHHC	26.47	30243723
2103	Phosphorylation	GSTGSSLSVSVRDAE CCCCCCEEEEHHCHH	17.81	30278072
2105	Phosphorylation	TGSSLSVSVRDAEAQ CCCCEEEEHHCHHHH	14.20	23403867

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of BIG3_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of BIG3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BIG3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PTHY_HUMAN	PTH	physical	11551928
PHB2_HUMAN	PHB2	physical	19496786
PHB2_HUMAN	PHB2	physical	24051437
PHB2_HUMAN	PHB2	physical	26052702

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1651 AND SER-1991, ANDMASS SPECTROMETRY.
PubMed: 18691976