ATG4D_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ATG4D_HUMAN
• UniProt AC: Q86TL0
• Protein Name: Cysteine protease ATG4D
• Gene Name: ATG4D
• Organism: Homo sapiens (Human).
• Sequence Length: 474
• Subcellular Localization: Cysteine protease ATG4D: Cytoplasm .; Cysteine protease ATG4D, mitochondrial: Cytoplasm . Mitochondrion matrix . Imported into mitochondrial matrix after cleavage by CASP3 during oxidative stress and cell death.
• Protein Description: Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.; Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability..
• Protein Sequence: MNSVSPAAAQYRSSSPEDARRRPEARRPRGPRGPDPNGLGPSGASGPALGSPGAGPSEPDEVDKFKAKFLTAWNNVKYGWVVKSRTSFSKISSIHLCGRRYRFEGEGDIQRFQRDFVSRLWLTYRRDFPPLPGGCLTSDCGWGCMLRSGQMMLAQGLLLHFLPRDWTWAEGMGLGPPELSGSASPSRYHGPARWMPPRWAQGAPELEQERRHRQIVSWFADHPRAPFGLHRLVELGQSSGKKAGDWYGPSLVAHILRKAVESCSDVTRLVVYVSQDCTVYKADVARLVARPDPTAEWKSVVILVPVRLGGETLNPVYVPCVKELLRCELCLGIMGGKPRHSLYFIGYQDDFLLYLDPHYCQPTVDVSQADFPLESFHCTSPRKMAFAKMDPSCTVGFYAGDRKEFETLCSELTRVLSSSSATERYPMFTLAEGHAQDHSLDDLCSQLAQPTLRLPRTGRLLRAKRPSSEDFVFL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MNSVSPAAAQ -----CCCCCHHHHH	23.75	22199227
5	Phosphorylation	---MNSVSPAAAQYR ---CCCCCHHHHHHH	14.51	22199227
13	Phosphorylation	PAAAQYRSSSPEDAR HHHHHHHCCCHHHHH	30.18	28102081
14	Phosphorylation	AAAQYRSSSPEDARR HHHHHHCCCHHHHHH	40.70	28102081
15	Phosphorylation	AAQYRSSSPEDARRR HHHHHCCCHHHHHHC	33.29	28102081
45	Phosphorylation	GLGPSGASGPALGSP CCCCCCCCCCCCCCC	49.16	23532336
51	Phosphorylation	ASGPALGSPGAGPSE CCCCCCCCCCCCCCC	22.47	23532336
64	Ubiquitination	SEPDEVDKFKAKFLT CCCCHHHHHHHHHHH	54.67	-
66	Ubiquitination	PDEVDKFKAKFLTAW CCHHHHHHHHHHHHH	57.30	-
68	Ubiquitination	EVDKFKAKFLTAWNN HHHHHHHHHHHHHCC	41.31	-
71	O-linked_Glycosylation	KFKAKFLTAWNNVKY HHHHHHHHHHCCCCE	32.53	30379171
87	Phosphorylation	WVVKSRTSFSKISSI EEEECCCCCCCCCEE	26.81	24719451
92	Phosphorylation	RTSFSKISSIHLCGR CCCCCCCCEEEECCC	27.29	23312004
93	Phosphorylation	TSFSKISSIHLCGRR CCCCCCCEEEECCCE	19.94	23312004
101	Phosphorylation	IHLCGRRYRFEGEGD EEECCCEEECCCCCH	20.49	19664994
167	Phosphorylation	HFLPRDWTWAEGMGL HHCCCCCHHHCCCCC	21.06	-
180	Phosphorylation	GLGPPELSGSASPSR CCCCCHHCCCCCCCC	29.10	24719451
182	Phosphorylation	GPPELSGSASPSRYH CCCHHCCCCCCCCCC	23.38	28348404
184	Phosphorylation	PELSGSASPSRYHGP CHHCCCCCCCCCCCC	25.71	28348404
241	Ubiquitination	ELGQSSGKKAGDWYG HHHHCCCCCCCCCCC	41.20	21906983
258	Ubiquitination	LVAHILRKAVESCSD HHHHHHHHHHHCCCC	54.03	-
264	Phosphorylation	RKAVESCSDVTRLVV HHHHHCCCCCCEEEE	44.38	19664994
298	Ubiquitination	PDPTAEWKSVVILVP CCCCCCCEEEEEEEE	25.52	-
322	Ubiquitination	PVYVPCVKELLRCEL CCHHHHHHHHHCCCH	49.73	-
388	Ubiquitination	PRKMAFAKMDPSCTV CCCEEEECCCCCCEE	37.06	-
398	Phosphorylation	PSCTVGFYAGDRKEF CCCEEEEEECCHHHH	12.07	22817900
403	Ubiquitination	GFYAGDRKEFETLCS EEEECCHHHHHHHHH	72.06	-
407	Phosphorylation	GDRKEFETLCSELTR CCHHHHHHHHHHHHH	38.08	24719451
410	Phosphorylation	KEFETLCSELTRVLS HHHHHHHHHHHHHHC	38.46	24719451
464	Ubiquitination	TGRLLRAKRPSSEDF CCCCEECCCCCCCCC	59.41	-
467	Phosphorylation	LLRAKRPSSEDFVFL CEECCCCCCCCCCCC	50.85	25159151
468	Phosphorylation	LRAKRPSSEDFVFL- EECCCCCCCCCCCC-	43.82	22617229

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ATG4D_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ATG4D_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ATG4D_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GBRL2_HUMAN	GABARAPL2	physical	19549685
GBRL1_HUMAN	GABARAPL1	physical	19549685

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.