ATG4A_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ATG4A_HUMAN
• UniProt AC: Q8WYN0
• Protein Name: Cysteine protease ATG4A
• Gene Name: ATG4A
• Organism: Homo sapiens (Human).
• Sequence Length: 398
• Subcellular Localization: Cytoplasm.
• Protein Description: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms..
• Protein Sequence: MESVLSKYEDQITIFTDYLEEYPDTDELVWILGKQHLLKTEKSKLLSDISARLWFTYRRKFSPIGGTGPSSDAGWGCMLRCGQMMLAQALICRHLGRDWSWEKQKEQPKEYQRILQCFLDRKDCCYSIHQMAQMGVGEGKSIGEWFGPNTVAQVLKKLALFDEWNSLAVYVSMDNTVVIEDIKKMCRVLPLSADTAGDRPPDSLTASNQSKGTSAYCSAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALGGKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEENGTVNDQTFHCLQSPQRMNILNLDPSVALGFFCKEEKDFDNWCSLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEEFDLEEDFEILSV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
47	Phosphorylation	TEKSKLLSDISARLW HHHHHHHHHHHHHHH	43.25	17924679
100	Phosphorylation	RHLGRDWSWEKQKEQ HHHCCCCCHHHHHHC	29.64	-
111	Phosphorylation	QKEQPKEYQRILQCF HHHCCHHHHHHHHHH	14.82	26074081
192	Phosphorylation	MCRVLPLSADTAGDR HHCCCCCCCCCCCCC	23.35	30576142
195	Phosphorylation	VLPLSADTAGDRPPD CCCCCCCCCCCCCCC	32.05	30576142
203	Phosphorylation	AGDRPPDSLTASNQS CCCCCCCCCCCCCCC	33.13	28555341
205	Phosphorylation	DRPPDSLTASNQSKG CCCCCCCCCCCCCCC	31.80	27251275
216	Phosphorylation	QSKGTSAYCSAWKPL CCCCCHHCCCCCCCE	6.16	30576142
328	Ubiquitination	GFFCKEEKDFDNWCS HCCCCCCCCCCCHHH	65.86	-
339	Ubiquitination	NWCSLVQKEILKENL CHHHHHHHHHHHHHH	38.96	-
343	Ubiquitination	LVQKEILKENLRMFE HHHHHHHHHHHHHHH	50.66	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ATG4A_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ATG4A_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ATG4A_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ATGA1_HUMAN	ATG101	physical	24100292
GBRL2_HUMAN	GABARAPL2	physical	12473658

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Improved titanium dioxide enrichment of phosphopeptides from HeLacells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.";
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.;
J. Proteome Res. 6:4150-4162(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, AND MASSSPECTROMETRY.
PubMed: 17924679