dbPTM

AT1A2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AT1A2_MOUSE
UniProt AC	Q6PIE5
Protein Name	Sodium/potassium-transporting ATPase subunit alpha-2
Gene Name	Atp1a2
Organism	Mus musculus (Mouse).
Sequence Length	1020
Subcellular Localization	Membrane Multi-pass membrane protein. Cell membrane Multi-pass membrane protein.
Protein Description	This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity)..
Protein Sequence	MGRGAGREYSPAATTAENGGGKKKQKEKELDELKKEVAMDDHKLSLDELGRKYQVDLSKGLTNQRAQDILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGALLCFLAYGILAAMEDEPSNDNLYLGIVLAAVVIVTGCFSYYQEAKSSKIMDSFKNMVPQQALVIREGEKMQINAEEVVVGDLVEVKGGDRVPADLRIISSHGCKVDNSSLTGESEPQTRSPEFTHENPLETRNICFFSTNCVEGTARGIVIATGDRTVMGRIATLASGLEVGQTPIAMEIEHFIQLITGVAVFLGVSFFVLSLILGYSWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGATFDKRSPTWTALSRIAGLCNRAVFKAGQENISVSKRDTAGDASESALLKCIELSCGSVRKMRDRNPKVAEIPFNSTNKYQLSIHEREDSPQSHVLVMKGAPERILDRCSTILVQGKEIPLDKEMQDAFQNAYMELGGLGERVLGFCQLNLPSGKFPRGFKFDTDELNFPTEKLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPREAKACVVHGSDLKDMTSEQLDEILRDHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGIAMGISGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEAAESDIMKRQPRNSQTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPSRLLGIRLDWDDRTTNDLEDSYGQEWTYEQRKVVEFTCHTAFFASIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLLEETALAAFLSYCPGMGVALRMYPLKVTWWFCAFPYSLLIFIYDEVRKLILRRYPGGWVEKETYY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	GRGAGREYSPAATTA CCCCCCCCCCCCCCC	20.70	25521595
10	Phosphorylation	RGAGREYSPAATTAE CCCCCCCCCCCCCCC	11.87	25521595
14	Phosphorylation	REYSPAATTAENGGG CCCCCCCCCCCCCCC	28.75	20415495
15	Phosphorylation	EYSPAATTAENGGGK CCCCCCCCCCCCCCC	27.52	20415495
28	Ubiquitination	GKKKQKEKELDELKK CCCCHHHHHHHHHHH	71.29	-
35	Ubiquitination	KELDELKKEVAMDDH HHHHHHHHHHCCCCC	70.78	-
43	Ubiquitination	EVAMDDHKLSLDELG HHCCCCCCCCHHHHC	46.92	-
45	Phosphorylation	AMDDHKLSLDELGRK CCCCCCCCHHHHCHH	38.28	29899451
52	Ubiquitination	SLDELGRKYQVDLSK CHHHHCHHHCCCCCC	37.93	-
58	Phosphorylation	RKYQVDLSKGLTNQR HHHCCCCCCCCCCHH	21.88	25521595
59	Ubiquitination	KYQVDLSKGLTNQRA HHCCCCCCCCCCHHH	66.30	-
79	Phosphorylation	RDGPNALTPPPTTPE CCCCCCCCCCCCCHH	31.19	22817900
83	Phosphorylation	NALTPPPTTPEWVKF CCCCCCCCCHHHHHH	61.84	20415495
84	Phosphorylation	ALTPPPTTPEWVKFC CCCCCCCCHHHHHHH	25.61	25521595
91	S-palmitoylation	TPEWVKFCRQLFGGF CHHHHHHHHHHHCHH	1.94	28680068
124	Phosphorylation	AAMEDEPSNDNLYLG HHCCCCCCCCCHHHH	55.56	-
154	Ubiquitination	YQEAKSSKIMDSFKN HHHHHHCHHHHHHHH	49.49	-
160	Ubiquitination	SKIMDSFKNMVPQQA CHHHHHHHHCCCCEE	48.35	-
175	Ubiquitination	LVIREGEKMQINAEE EEEECCCEEEECCEE	47.14	-
192	Ubiquitination	VGDLVEVKGGDRVPA EECEEEEECCCCCCC	43.61	-
205	Phosphorylation	PADLRIISSHGCKVD CCCEEEEECCCCEEC	17.57	22817900
206	Phosphorylation	ADLRIISSHGCKVDN CCEEEEECCCCEECC	17.31	29899451
209	S-nitrosocysteine	RIISSHGCKVDNSSL EEEECCCCEECCCCC	3.04	-
209	S-nitrosylation	RIISSHGCKVDNSSL EEEECCCCEECCCCC	3.04	24895380
210	Ubiquitination	IISSHGCKVDNSSLT EEECCCCEECCCCCC	58.16	-
214	Phosphorylation	HGCKVDNSSLTGESE CCCEECCCCCCCCCC	23.43	30635358
215	Phosphorylation	GCKVDNSSLTGESEP CCEECCCCCCCCCCC	35.88	25521595
217	Phosphorylation	KVDNSSLTGESEPQT EECCCCCCCCCCCCC	40.63	27742792
220	Phosphorylation	NSSLTGESEPQTRSP CCCCCCCCCCCCCCC	56.79	25521595
224	Phosphorylation	TGESEPQTRSPEFTH CCCCCCCCCCCCCCC	43.78	25521595
226	Phosphorylation	ESEPQTRSPEFTHEN CCCCCCCCCCCCCCC	31.97	25521595
241	S-nitrosylation	PLETRNICFFSTNCV CCCCCCEEEEECCCC	3.00	24895380
247	S-nitrosylation	ICFFSTNCVEGTARG EEEEECCCCCCCCCE	2.74	24895380
270	Phosphorylation	TVMGRIATLASGLEV CHHHHHHHHHHCCCC	22.08	-
273	Phosphorylation	GRIATLASGLEVGQT HHHHHHHHCCCCCCC	47.20	-
357	Ubiquitination	ARKNCLVKNLEAVET HHCCCEECCHHHHHH	42.75	-
364	Phosphorylation	KNLEAVETLGSTSTI CCHHHHHHHCCCCCC	29.80	23140645
367	Phosphorylation	EAVETLGSTSTICSD HHHHHHCCCCCCCCC	23.46	25521595
368	Phosphorylation	AVETLGSTSTICSDK HHHHHCCCCCCCCCC	28.26	24925903
369	Phosphorylation	VETLGSTSTICSDKT HHHHCCCCCCCCCCC	19.92	23140645
370	Phosphorylation	ETLGSTSTICSDKTG HHHCCCCCCCCCCCC	26.54	24925903
372	S-nitrosocysteine	LGSTSTICSDKTGTL HCCCCCCCCCCCCCC	4.33	-
372	S-nitrosylation	LGSTSTICSDKTGTL HCCCCCCCCCCCCCC	4.33	22588120
373	Phosphorylation	GSTSTICSDKTGTLT CCCCCCCCCCCCCCC	37.99	25521595
375	Acetylation	TSTICSDKTGTLTQN CCCCCCCCCCCCCCC	32.34	6569671
375	Ubiquitination	TSTICSDKTGTLTQN CCCCCCCCCCCCCCC	32.34	-
376	Phosphorylation	STICSDKTGTLTQNR CCCCCCCCCCCCCCC	40.15	22324799
378	Phosphorylation	ICSDKTGTLTQNRMT CCCCCCCCCCCCCEE	31.68	22324799
380	Phosphorylation	SDKTGTLTQNRMTVA CCCCCCCCCCCEEEE	24.52	24925903
405	Phosphorylation	ADTTEDQSGATFDKR CCCCCCCCCCCCCCC	42.59	29899451
411	Ubiquitination	QSGATFDKRSPTWTA CCCCCCCCCCCHHHH	51.05	-
413	Phosphorylation	GATFDKRSPTWTALS CCCCCCCCCHHHHHH	32.21	22817900
432	Ubiquitination	LCNRAVFKAGQENIS HHHHHHHHCCHHCCC	45.04	-
439	Phosphorylation	KAGQENISVSKRDTA HCCHHCCCCCCCCCC	31.74	25521595
441	Phosphorylation	GQENISVSKRDTAGD CHHCCCCCCCCCCCC	18.38	22324799
442	Ubiquitination	QENISVSKRDTAGDA HHCCCCCCCCCCCCC	52.73	-
445	Phosphorylation	ISVSKRDTAGDASES CCCCCCCCCCCCCHH	36.25	23984901
450	Phosphorylation	RDTAGDASESALLKC CCCCCCCCHHHHHHH	36.50	25521595
452	Phosphorylation	TAGDASESALLKCIE CCCCCCHHHHHHHHH	23.01	25521595
457	S-nitrosylation	SESALLKCIELSCGS CHHHHHHHHHHHCCC	2.70	24895380
457	S-palmitoylation	SESALLKCIELSCGS CHHHHHHHHHHHCCC	2.70	28680068
461	Phosphorylation	LLKCIELSCGSVRKM HHHHHHHHCCCHHHH	12.02	22817900
462	S-nitrosylation	LKCIELSCGSVRKMR HHHHHHHCCCHHHHH	8.02	24895380
464	Phosphorylation	CIELSCGSVRKMRDR HHHHHCCCHHHHHCC	24.07	22324799
474	Ubiquitination	KMRDRNPKVAEIPFN HHHCCCCCEEECCCC	58.59	-
482	Phosphorylation	VAEIPFNSTNKYQLS EEECCCCCCCCEEEE	33.46	22324799
483	Phosphorylation	AEIPFNSTNKYQLSI EECCCCCCCCEEEEE	36.51	25521595
496	Phosphorylation	SIHEREDSPQSHVLV EEEECCCCCCCEEEE	21.78	25521595
499	Phosphorylation	EREDSPQSHVLVMKG ECCCCCCCEEEEECC	20.96	25521595
505	Ubiquitination	QSHVLVMKGAPERIL CCEEEEECCCHHHHH	44.43	-
515	S-nitrosylation	PERILDRCSTILVQG HHHHHHHCCEEEECC	4.14	24895380
516	Phosphorylation	ERILDRCSTILVQGK HHHHHHCCEEEECCC	21.26	22324799
517	Phosphorylation	RILDRCSTILVQGKE HHHHHCCEEEECCCC	23.19	25521595
523	Ubiquitination	STILVQGKEIPLDKE CEEEECCCCCCCCHH	35.07	-
529	Ubiquitination	GKEIPLDKEMQDAFQ CCCCCCCHHHHHHHH	63.87	-
539	Phosphorylation	QDAFQNAYMELGGLG HHHHHHHHHHHCCHH	9.93	29899451
553	S-nitrosylation	GERVLGFCQLNLPSG HHHHHEEEEECCCCC	4.22	24895380
559	Phosphorylation	FCQLNLPSGKFPRGF EEEECCCCCCCCCCC	58.75	25521595
561	Ubiquitination	QLNLPSGKFPRGFKF EECCCCCCCCCCCCC	57.44	-
567	Ubiquitination	GKFPRGFKFDTDELN CCCCCCCCCCHHHCC	45.19	-
570	Phosphorylation	PRGFKFDTDELNFPT CCCCCCCHHHCCCCH	34.21	-
587	Phosphorylation	LCFVGLMSMIDPPRA HHHHHHHHCCCCCHH	20.03	-
602	Ubiquitination	AVPDAVGKCRSAGIK CCCCHHHHHHHCCCE	21.63	-
605	Phosphorylation	DAVGKCRSAGIKVIM CHHHHHHHCCCEEEE	40.07	29899451
614	O-linked_Glycosylation	GIKVIMVTGDHPITA CCEEEEEECCCCCCH	21.36	51462353
614	Phosphorylation	GIKVIMVTGDHPITA CCEEEEEECCCCCCH	21.36	28638064
620	Phosphorylation	VTGDHPITAKAIAKG EECCCCCCHHHHHHC	26.93	-
622	Ubiquitination	GDHPITAKAIAKGVG CCCCCCHHHHHHCCE	31.51	-
626	Ubiquitination	ITAKAIAKGVGIISE CCHHHHHHCCEEEEC	48.27	-
632	Phosphorylation	AKGVGIISEGNETVE HHCCEEEECCCCCHH	37.21	29899451
637	Phosphorylation	IISEGNETVEDIAAR EEECCCCCHHHHHHH	32.94	22324799
650	O-linked_Glycosylation	ARLNIPVSQVNPREA HHCCCCHHHCCHHHC	24.05	179353
650	Phosphorylation	ARLNIPVSQVNPREA HHCCCCHHHCCHHHC	24.05	27180971
658	Ubiquitination	QVNPREAKACVVHGS HCCHHHCCEEEEECH	37.25	-
660	S-nitrosocysteine	NPREAKACVVHGSDL CHHHCCEEEEECHHH	3.02	-
660	S-nitrosylation	NPREAKACVVHGSDL CHHHCCEEEEECHHH	3.02	24895380
665	Phosphorylation	KACVVHGSDLKDMTS CEEEEECHHHHCCCH	25.54	22324799
668	Ubiquitination	VVHGSDLKDMTSEQL EEECHHHHCCCHHHH	50.90	-
671	Phosphorylation	GSDLKDMTSEQLDEI CHHHHCCCHHHHHHH	38.97	20495213
672	Phosphorylation	SDLKDMTSEQLDEIL HHHHCCCHHHHHHHH	19.39	20495213
683	Phosphorylation	DEILRDHTEIVFART HHHHHHCCEEEEEEC	31.53	29899451
695	Ubiquitination	ARTSPQQKLIIVEGC EECCCCCEEEEEECC	36.08	-
702	S-nitrosocysteine	KLIIVEGCQRQGAIV EEEEEECCCCCCCEE	1.58	-
702	S-nitrosylation	KLIIVEGCQRQGAIV EEEEEECCCCCCCEE	1.58	24895380
712	Phosphorylation	QGAIVAVTGDGVNDS CCCEEEEECCCCCCC	21.59	24925903
719	Phosphorylation	TGDGVNDSPALKKAD ECCCCCCCHHHHHCC	13.58	25521595
723	Acetylation	VNDSPALKKADIGIA CCCCHHHHHCCEEEE	48.28	22902405
723	Ubiquitination	VNDSPALKKADIGIA CCCCHHHHHCCEEEE	48.28	-
724	Ubiquitination	NDSPALKKADIGIAM CCCHHHHHCCEEEEC	52.74	-
770	Ubiquitination	RLIFDNLKKSIAYTL EEEECCHHHHHHHHH	51.21	-
826	Phosphorylation	LAYEAAESDIMKRQP HHHHHHHHHHHHCCC	27.92	-
840	Ubiquitination	PRNSQTDKLVNERLI CCCCHHHHHHHHHHH	58.69	-
891	Phosphorylation	RLDWDDRTTNDLEDS ECCCCCCCCCCCHHH	36.62	17203969
892	Phosphorylation	LDWDDRTTNDLEDSY CCCCCCCCCCCHHHC	27.85	17203969
940	Phosphorylation	ICKTRRNSVFQQGMK EECCCCCHHHHHHHC	23.61	19060867
947	Ubiquitination	SVFQQGMKNKILIFG HHHHHHHCCCHHEEC	63.01	-
1016	Ubiquitination	YPGGWVEKETYY--- CCCCCEEEEECC---	45.82	-
1020	Phosphorylation	WVEKETYY------- CEEEEECC-------	21.35	18034455

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
940	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AT1A2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AT1A2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
NAC1_MOUSE	Slc8a1	physical	15485817

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AT1A2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-650, AND MASSSPECTROMETRY.	17114649 [Abstract]