dbPTM

AT1A1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AT1A1_MOUSE
UniProt AC	Q8VDN2
Protein Name	Sodium/potassium-transporting ATPase subunit alpha-1
Gene Name	Atp1a1
Organism	Mus musculus (Mouse).
Sequence Length	1023
Subcellular Localization	Cell membrane, sarcolemma Multi-pass membrane protein .
Protein Description	This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity)..
Protein Sequence	MGKGVGRDKYEPAAVSEHGDKKGKKAKKERDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTPARAAEILARDGPNALTPPPTTPEWVKFCRQLFGGFSMLLWIGAILCFLAYGIRSATEEEPPNDDLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMSINAEDVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSPDFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAEEIEHFIHLITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWFALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIEVCCGSVMEMREKYSKIVEIPFNSTNKYQLSIHKNPNASEPKHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLLLPDEQFPEGFQFDTDDVNFPVDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVNQVNPRDAKACVVHGSDLKDMTSEELDDILRYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIVGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPFHLLGIRETWDDRWVNDVEDSYGQQWTYEQRKIVEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGAALRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Acetylation	GKGVGRDKYEPAAVS CCCCCCCCCCCCHHH	50.59	23806337
9	Ubiquitination	GKGVGRDKYEPAAVS CCCCCCCCCCCCHHH	50.59	22790023
10	Phosphorylation	KGVGRDKYEPAAVSE CCCCCCCCCCCHHHC	31.28	25159016
16	Phosphorylation	KYEPAAVSEHGDKKG CCCCCHHHCCCCCCC	21.10	25521595
21	Acetylation	AVSEHGDKKGKKAKK HHHCCCCCCCHHCHH	68.93	23806337
21	Ubiquitination	AVSEHGDKKGKKAKK HHHCCCCCCCHHCHH	68.93	22790023
22	Ubiquitination	VSEHGDKKGKKAKKE HHCCCCCCCHHCHHH	79.35	-
36	Ubiquitination	ERDMDELKKEVSMDD HHCHHHHHHHHCCCC	44.85	22790023
37	Ubiquitination	RDMDELKKEVSMDDH HCHHHHHHHHCCCCC	75.79	22790023
40	Phosphorylation	DELKKEVSMDDHKLS HHHHHHHCCCCCCCC	20.00	25521595
45	Ubiquitination	EVSMDDHKLSLDELH HHCCCCCCCCHHHHH	46.92	22790023
47	Phosphorylation	SMDDHKLSLDELHRK CCCCCCCCHHHHHHH	38.28	25521595
54	Ubiquitination	SLDELHRKYGTDLSR CHHHHHHHHCCCCCC	36.97	22790023
55	Phosphorylation	LDELHRKYGTDLSRG HHHHHHHHCCCCCCC	25.80	22817900
57	Phosphorylation	ELHRKYGTDLSRGLT HHHHHHCCCCCCCCC	30.58	22817900
60	Phosphorylation	RKYGTDLSRGLTPAR HHHCCCCCCCCCHHH	27.41	23737553
81	Phosphorylation	RDGPNALTPPPTTPE HCCCCCCCCCCCCHH	31.19	22817900
85	Phosphorylation	NALTPPPTTPEWVKF CCCCCCCCCHHHHHH	61.84	20415495
86	Phosphorylation	ALTPPPTTPEWVKFC CCCCCCCCHHHHHHH	25.61	25521595
93	S-palmitoylation	TPEWVKFCRQLFGGF CHHHHHHHHHHHCHH	1.94	28680068
156	Ubiquitination	YQEAKSSKIMESFKN HHHHHHHHHHHHHHH	53.84	22790023
160	Phosphorylation	KSSKIMESFKNMVPQ HHHHHHHHHHHCCCC	24.92	22324799
162	Ubiquitination	SKIMESFKNMVPQQA HHHHHHHHHCCCCCE	54.11	22790023
177	Ubiquitination	LVIRNGEKMSINAED EEEECCCCCEECHHH	39.07	22790023
179	Phosphorylation	IRNGEKMSINAEDVV EECCCCCEECHHHCE	24.61	29899451
194	Ubiquitination	VGDLVEVKGGDRIPA EECEEEEECCCCCCC	43.61	22790023
207	Phosphorylation	PADLRIISANGCKVD CCCEEEEECCCCEEC	17.20	22324799
211	S-nitrosocysteine	RIISANGCKVDNSSL EEEECCCCEECCCCC	3.78	-
211	Glutathionylation	RIISANGCKVDNSSL EEEECCCCEECCCCC	3.78	24333276
211	S-nitrosylation	RIISANGCKVDNSSL EEEECCCCEECCCCC	3.78	24895380
212	Ubiquitination	IISANGCKVDNSSLT EEECCCCEECCCCCC	54.76	22790023
216	Phosphorylation	NGCKVDNSSLTGESE CCCEECCCCCCCCCC	23.43	25521595
217	Phosphorylation	GCKVDNSSLTGESEP CCEECCCCCCCCCCC	35.88	25521595
219	Phosphorylation	KVDNSSLTGESEPQT EECCCCCCCCCCCCC	40.63	24925903
222	Phosphorylation	NSSLTGESEPQTRSP CCCCCCCCCCCCCCC	56.79	25521595
226	Phosphorylation	TGESEPQTRSPDFTN CCCCCCCCCCCCCCC	43.78	25521595
228	Phosphorylation	ESEPQTRSPDFTNEN CCCCCCCCCCCCCCC	32.01	25521595
232	Phosphorylation	QTRSPDFTNENPLET CCCCCCCCCCCCCCC	49.34	25521595
239	Phosphorylation	TNENPLETRNIAFFS CCCCCCCCCCEEEEE	36.38	24925903
246	Phosphorylation	TRNIAFFSTNCVEGT CCCEEEEECCCCCCC	16.56	29899451
247	Phosphorylation	RNIAFFSTNCVEGTA CCEEEEECCCCCCCC	27.72	25521595
249	S-nitrosocysteine	IAFFSTNCVEGTARG EEEEECCCCCCCCCE	2.74	-
249	S-nitrosylation	IAFFSTNCVEGTARG EEEEECCCCCCCCCE	2.74	24895380
249	S-palmitoylation	IAFFSTNCVEGTARG EEEEECCCCCCCCCE	2.74	28526873
260	Phosphorylation	TARGIVVYTGDRTVM CCCEEEEECCCCCHH	8.29	25177544
261	Phosphorylation	ARGIVVYTGDRTVMG CCEEEEECCCCCHHH	22.46	22499769
265	Phosphorylation	VVYTGDRTVMGRIAT EEECCCCCHHHHHHH	21.42	25367039
275	Phosphorylation	GRIATLASGLEGGQT HHHHHHHHHCCCCCC	47.20	22817900
354	Ubiquitination	TAKRMARKNCLVKNL HHHHHHHCCCEECCH	42.34	22790023
359	Ubiquitination	ARKNCLVKNLEAVET HHCCCEECCHHHHHH	42.75	22790023
366	Phosphorylation	KNLEAVETLGSTSTI CCHHHHHHHCCCCCC	29.80	23140645
369	Phosphorylation	EAVETLGSTSTICSD HHHHHHCCCCCCCCC	23.46	25521595
370	Phosphorylation	AVETLGSTSTICSDK HHHHHCCCCCCCCCC	28.26	24925903
371	Phosphorylation	VETLGSTSTICSDKT HHHHCCCCCCCCCCC	19.92	23140645
372	Phosphorylation	ETLGSTSTICSDKTG HHHCCCCCCCCCCCC	26.54	24925903
374	S-nitrosocysteine	LGSTSTICSDKTGTL HCCCCCCCCCCCCCC	4.33	-
374	Glutathionylation	LGSTSTICSDKTGTL HCCCCCCCCCCCCCC	4.33	24333276
374	S-nitrosylation	LGSTSTICSDKTGTL HCCCCCCCCCCCCCC	4.33	24895380
374	S-palmitoylation	LGSTSTICSDKTGTL HCCCCCCCCCCCCCC	4.33	28526873
375	Phosphorylation	GSTSTICSDKTGTLT CCCCCCCCCCCCCCC	37.99	25521595
377	Acetylation	TSTICSDKTGTLTQN CCCCCCCCCCCCCCC	32.34	6569665
377	Ubiquitination	TSTICSDKTGTLTQN CCCCCCCCCCCCCCC	32.34	22790023
378	Phosphorylation	STICSDKTGTLTQNR CCCCCCCCCCCCCCC	40.15	22324799
380	Phosphorylation	ICSDKTGTLTQNRMT CCCCCCCCCCCCCEE	31.68	22324799
382	Phosphorylation	SDKTGTLTQNRMTVA CCCCCCCCCCCEEEE	24.52	24925903
402	Phosphorylation	NQIHEADTTENQSGV CCCCCCCCCCCCCCC	42.95	25367039
403	Phosphorylation	QIHEADTTENQSGVS CCCCCCCCCCCCCCC	33.22	25367039
407	Phosphorylation	ADTTENQSGVSFDKT CCCCCCCCCCCCCHH	53.39	25521595
410	Phosphorylation	TENQSGVSFDKTSAT CCCCCCCCCCHHHHH	31.11	29899451
413	Ubiquitination	QSGVSFDKTSATWFA CCCCCCCHHHHHHHH	42.23	22790023
414	Phosphorylation	SGVSFDKTSATWFAL CCCCCCHHHHHHHHH	25.99	25367039
415	Phosphorylation	GVSFDKTSATWFALS CCCCCHHHHHHHHHH	29.46	25367039
417	Phosphorylation	SFDKTSATWFALSRI CCCHHHHHHHHHHHH	22.38	25367039
422	Phosphorylation	SATWFALSRIAGLCN HHHHHHHHHHHHHHH	20.43	25367039
428	S-nitrosocysteine	LSRIAGLCNRAVFQA HHHHHHHHHHHHHHH	2.95	-
428	S-nitrosylation	LSRIAGLCNRAVFQA HHHHHHHHHHHHHHH	2.95	20925432
444	Ubiquitination	QENLPILKRAVAGDA HHCCHHHHHHHCCCC	38.74	22790023
452	Phosphorylation	RAVAGDASESALLKC HHHCCCCCHHHHHHH	36.50	25521595
454	Phosphorylation	VAGDASESALLKCIE HCCCCCHHHHHHHHH	23.01	22324799
459	S-nitrosocysteine	SESALLKCIEVCCGS CHHHHHHHHHHHHHH	3.02	-
459	S-nitrosylation	SESALLKCIEVCCGS CHHHHHHHHHHHHHH	3.02	20925432
476	Ubiquitination	EMREKYSKIVEIPFN HHHHHHHCCEEECCC	47.91	22790023
484	Phosphorylation	IVEIPFNSTNKYQLS CEEECCCCCCCEEEE	33.46	25521595
485	Phosphorylation	VEIPFNSTNKYQLSI EEECCCCCCCEEEEE	36.51	25521595
487	Acetylation	IPFNSTNKYQLSIHK ECCCCCCCEEEEEEC	34.17	23236377
487	Malonylation	IPFNSTNKYQLSIHK ECCCCCCCEEEEEEC	34.17	26320211
487	Ubiquitination	IPFNSTNKYQLSIHK ECCCCCCCEEEEEEC	34.17	-
494	Acetylation	KYQLSIHKNPNASEP CEEEEEECCCCCCCC	72.87	22733758
494	Malonylation	KYQLSIHKNPNASEP CEEEEEECCCCCCCC	72.87	26320211
494	Ubiquitination	KYQLSIHKNPNASEP CEEEEEECCCCCCCC	72.87	27667366
499	Phosphorylation	IHKNPNASEPKHLLV EECCCCCCCCCEEEE	62.16	25521595
502	Acetylation	NPNASEPKHLLVMKG CCCCCCCCEEEEECC	42.72	23954790
502	Ubiquitination	NPNASEPKHLLVMKG CCCCCCCCEEEEECC	42.72	22790023
508	Acetylation	PKHLLVMKGAPERIL CCEEEEECCCHHHHH	44.43	22733758
508	Ubiquitination	PKHLLVMKGAPERIL CCEEEEECCCHHHHH	44.43	22790023
518	S-nitrosocysteine	PERILDRCSSILLHG HHHHHHHHHHHHHCC	3.60	-
518	S-nitrosylation	PERILDRCSSILLHG HHHHHHHHHHHHHCC	3.60	21278135
518	S-palmitoylation	PERILDRCSSILLHG HHHHHHHHHHHHHCC	3.60	26165157
526	Ubiquitination	SSILLHGKEQPLDEE HHHHHCCCCCCCCHH	42.29	22790023
535	Acetylation	QPLDEELKDAFQNAY CCCCHHHHHHHHHHH	50.74	22733758
542	Phosphorylation	KDAFQNAYLELGGLG HHHHHHHHHHHCCHH	14.30	23140645
605	Ubiquitination	AVPDAVGKCRSAGIK CCCCHHHHHHHCCCE	21.63	22790023
608	Phosphorylation	DAVGKCRSAGIKVIM CHHHHHHHCCCEEEE	40.07	29899451
617	O-linked_Glycosylation	GIKVIMVTGDHPITA CCEEEEEECCCCCCH	21.36	51462345
617	Phosphorylation	GIKVIMVTGDHPITA CCEEEEEECCCCCCH	21.36	28638064
623	Phosphorylation	VTGDHPITAKAIAKG EECCCCCCHHHHHHC	26.93	-
625	Ubiquitination	GDHPITAKAIAKGVG CCCCCCHHHHHHCCE	31.51	22790023
629	Ubiquitination	ITAKAIAKGVGIISE CCHHHHHHCCEEEEC	48.27	22790023
635	Phosphorylation	AKGVGIISEGNETVE HHCCEEEECCCCCHH	37.21	22324799
640	Phosphorylation	IISEGNETVEDIAAR EEECCCCCHHHHHHH	32.94	22324799
661	Acetylation	QVNPRDAKACVVHGS HCCHHHCCEEEEECH	46.75	23806337
661	Succinylation	QVNPRDAKACVVHGS HCCHHHCCEEEEECH	46.75	-
661	Succinylation	QVNPRDAKACVVHGS HCCHHHCCEEEEECH	46.75	23806337
661	Ubiquitination	QVNPRDAKACVVHGS HCCHHHCCEEEEECH	46.75	-
663	S-nitrosocysteine	NPRDAKACVVHGSDL CHHHCCEEEEECHHH	3.02	-
663	Glutathionylation	NPRDAKACVVHGSDL CHHHCCEEEEECHHH	3.02	24333276
663	S-nitrosylation	NPRDAKACVVHGSDL CHHHCCEEEEECHHH	3.02	22178444
668	Phosphorylation	KACVVHGSDLKDMTS CEEEEECHHHCCCCH	25.54	22324799
671	Ubiquitination	VVHGSDLKDMTSEEL EEECHHHCCCCHHHH	50.90	22790023
674	Phosphorylation	GSDLKDMTSEELDDI CHHHCCCCHHHHHHH	43.02	23737553
675	Phosphorylation	SDLKDMTSEELDDIL HHHCCCCHHHHHHHH	23.57	23737553
684	Phosphorylation	ELDDILRYHTEIVFA HHHHHHHHHHEEEEE	15.06	25521595
686	Phosphorylation	DDILRYHTEIVFART HHHHHHHHEEEEEEC	20.62	25195567
698	Ubiquitination	ARTSPQQKLIIVEGC EECCCCCEEEEEECC	36.08	22790023
705	S-nitrosocysteine	KLIIVEGCQRQGAIV EEEEEECCCCCCCEE	1.58	-
705	S-nitrosylation	KLIIVEGCQRQGAIV EEEEEECCCCCCCEE	1.58	22178444
705	S-palmitoylation	KLIIVEGCQRQGAIV EEEEEECCCCCCCEE	1.58	28526873
715	Phosphorylation	QGAIVAVTGDGVNDS CCCEEEEECCCCCCC	21.59	24925903
722	Phosphorylation	TGDGVNDSPALKKAD ECCCCCCCHHHHHCC	13.58	25521595
726	Acetylation	VNDSPALKKADIGVA CCCCHHHHHCCCEEE	48.28	130351
726	Ubiquitination	VNDSPALKKADIGVA CCCCHHHHHCCCEEE	48.28	22790023
727	Ubiquitination	NDSPALKKADIGVAM CCCHHHHHCCCEEEE	52.74	22790023
773	Ubiquitination	RLIFDNLKKSIAYTL EECCCCHHHHHHHHH	51.21	22790023
833	Ubiquitination	QAESDIMKRQPRNPK HHHHHHHHCCCCCCC	48.49	-
840	Ubiquitination	KRQPRNPKTDKLVNE HCCCCCCCHHHHHHH	73.27	22790023
843	Acetylation	PRNPKTDKLVNERLI CCCCCHHHHHHHHHH	60.61	23201123
843	Ubiquitination	PRNPKTDKLVNERLI CCCCCHHHHHHHHHH	60.61	22790023
943	Phosphorylation	ICKTRRNSVFQQGMK EECCCCCHHHHHHCC	23.61	29472430
950	Ubiquitination	SVFQQGMKNKILIFG HHHHHHCCCCEEEEE	63.01	22790023
1019	Ubiquitination	RPGGWVEKETYY--- CCCCCCCCCCCC---	45.82	22790023
1023	Phosphorylation	WVEKETYY------- CCCCCCCC-------	21.35	18034455

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
943	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
943	S	Phosphorylation		-
Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AT1A1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
NAC1_MOUSE	Slc8a1	physical	15485817

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AT1A1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217; THR-219; SER-228AND SER-452, AND MASS SPECTROMETRY.	17114649 [Abstract]
"Quantitative analysis of both protein expression and serine /threonine post-translational modifications through stable isotopelabeling with dithiothreitol."; Vosseller K., Hansen K.C., Chalkley R.J., Trinidad J.C., Wells L.,Hart G.W., Burlingame A.L.; Proteomics 5:388-398(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, AND MASSSPECTROMETRY.	15648052 [Abstract]
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-260, AND MASSSPECTROMETRY.	18034455 [Abstract]

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