dbPTM

ARHGI_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ARHGI_HUMAN
UniProt AC	Q6ZSZ5
Protein Name	Rho guanine nucleotide exchange factor 18
Gene Name	ARHGEF18
Organism	Homo sapiens (Human).
Sequence Length	1173
Subcellular Localization	Cytoplasm . Cytoplasm, cytoskeleton . Apical cell membrane .
Protein Description	Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells. [PubMed: 22006950]
Protein Sequence	MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALPGRPELSFYGSFPRKWSENVFLDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYELMQTEVHHVRTLKIMLKVYSRALQEELQFSSKAIGRLFPCADDLLETHSHFLARLKERRQESLEEGSDRNYVIQKIGDLLVQQFSGENGERMKEKYGVFCSGHNEAVSHYKLLLQQNKKFQNLIKKIGNFSIVRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTEDYEDLTQALNLIKDIISQVDAKVSECEKGQRLREIAGKMDLKSSSKLKNGLTFRKEDMLQRQLHLEGMLCWKTTSGRLKDILAILLTDVLLLLQEKDQKYVFASVDSKPPVISLQKLIVREVANEEKAMFLISASLQGPEMYEIYTSSKEDRNAWMAHIQRAVESCPDEEEGPFSLPEEERKVVEARATRLRDFQERLSMKDQLIAQSLLEKQQIYLEMAEMGGLEDLPQPRGLFRGGDPSETLQGELILKSAMSEIEGIQSLICRQLGSANGQAEDGGSSTGPPRRAETFAGYDCTNSPTKNGSFKKKVSSTDPRPRDWRGPPNSPDLKLSDSDIPGSSEESPQVVEAPGTESDPRLPTVLESELVQRIQTLSQLLLNLQAVIAHQDSYVETQRAAIQEREKQFRLQSTRGNLLLEQERQRNFEKQREERAALEKLQSQLRHEQQRWERERQWQHQELERAGARLQEREGEARQLRERLEQERAELERQRQAYQHDLERLREAQRAVERERERLELLRRLKKQNTAPGALPPDTLAEAQPPSHPPSFNGEGLEGPRVSMLPSGVGPEYAERPEVARRDSAPTENRLAKSDVPIQLLSATNQFQRQAAVQQQIPTKLAASTKGGKDKGGKSRGSQRWESSASFDLKQQLLLNKLMGKDESTSRNRRSLSPILPGRHSPAPPPDPGFPAPSPPPADSPSEGFSLKAGGTALLPGPPAPSPLPATPLSAKEDASKEDVIFF

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MVTVGTNILP -----CCEECCCCCC	25.69	29255136
6	Phosphorylation	--MVTVGTNILPSRP --CCEECCCCCCCCC	18.10	29255136
11	Phosphorylation	VGTNILPSRPAASAN ECCCCCCCCCCCCCC	47.66	29255136
16	Phosphorylation	LPSRPAASANTARED CCCCCCCCCCCHHHH	24.94	24043423
19	Phosphorylation	RPAASANTAREDAAL CCCCCCCCHHHHHHH	27.15	24043423
28	Phosphorylation	REDAALFSRRIPPRH HHHHHHHHCCCCCCC	22.84	24043423
71	Phosphorylation	DRHSALPGRPELSFY CCCCCCCCCCCCEEE	59.64	28450419
75	Phosphorylation	ALPGRPELSFYGSFP CCCCCCCCEEEECCC	4.80	26714015
83	Phosphorylation	SFYGSFPRKWSENVF EEEECCCCCCCCCCC	51.57	28450419
85	Phosphorylation	YGSFPRKWSENVFLD EECCCCCCCCCCCCC	16.50	28450419
87	Phosphorylation	SFPRKWSENVFLDNE CCCCCCCCCCCCCCH	57.19	28450419
89	Phosphorylation	PRKWSENVFLDNELL CCCCCCCCCCCCHHH	4.41	27251275
98	Phosphorylation	LDNELLTSKILSVLR CCCHHHHHHHHHHHC	20.05	30576142
102	Phosphorylation	LLTSKILSVLRPQSE HHHHHHHHHHCCCCC	23.68	24719451
124	Phosphorylation	LRYPTHFLSTNSVLA CCCCCHHCCCCHHHH	4.85	26552605
126	Phosphorylation	YPTHFLSTNSVLASV CCCHHCCCCHHHHHH	33.39	26552605
131	Phosphorylation	LSTNSVLASVTASLK CCCCHHHHHHHHHHH	10.37	28450419
135	Phosphorylation	SVLASVTASLKEHPR HHHHHHHHHHHCCCC	15.65	28450419
139	Phosphorylation	SVTASLKEHPRGTLL HHHHHHHCCCCCCCC	65.50	28450419
144	Phosphorylation	LKEHPRGTLLSDGSP HHCCCCCCCCCCCCC	26.30	30301811
146	Phosphorylation	EHPRGTLLSDGSPAL CCCCCCCCCCCCCCH	4.38	28450419
147	Phosphorylation	HPRGTLLSDGSPALS CCCCCCCCCCCCCHH	42.93	28450419
150	Phosphorylation	GTLLSDGSPALSRNV CCCCCCCCCCHHCCC	16.40	23401153
153	Phosphorylation	LSDGSPALSRNVGMT CCCCCCCHHCCCCCE	5.84	26657352
154	Phosphorylation	SDGSPALSRNVGMTV CCCCCCHHCCCCCEE	24.74	29978859
155	Phosphorylation	DGSPALSRNVGMTVS CCCCCHHCCCCCEEC	42.58	22115753
159	Phosphorylation	ALSRNVGMTVSQKGG CHHCCCCCEECCCCC	2.66	27251275
162	Phosphorylation	RNVGMTVSQKGGPQP CCCCCEECCCCCCCC	19.60	24247654
204	Phosphorylation	FKQTADDSLSLTSPN HHHCCCCCCCCCCCC	22.02	21712546
208	Phosphorylation	ADDSLSLTSPNTESI CCCCCCCCCCCCCCE	38.47	18669648
209	Phosphorylation	DDSLSLTSPNTESIF CCCCCCCCCCCCCEE	22.80	18669648
212	Phosphorylation	LSLTSPNTESIFVED CCCCCCCCCCEEEEC	34.20	18669648
263	Phosphorylation	EVVKRQDVLYELMQT HHHHHHHHHHHHHHH	4.67	24719451
265	Phosphorylation	VKRQDVLYELMQTEV HHHHHHHHHHHHHCC	13.58	24043423
270	Phosphorylation	VLYELMQTEVHHVRT HHHHHHHHCCHHHHH	26.47	24043423
328	Phosphorylation	LKERRQESLEEGSDR HHHHHHHHHHCCCCH	32.51	22985185
333	Phosphorylation	QESLEEGSDRNYVIQ HHHHHCCCCHHHHHH	36.36	26471730
359	Acetylation	GENGERMKEKYGVFC CCCCCHHHHHHCEEC	58.22	19824939
361	Acetylation	NGERMKEKYGVFCSG CCCHHHHHHCEECCC	41.61	19824947
376	Phosphorylation	HNEAVSHYKLLLQQN CHHHHHHHHHHHHHH	8.88	-
377	Acetylation	NEAVSHYKLLLQQNK HHHHHHHHHHHHHHH	27.51	19824955
412	Phosphorylation	QECILLVTQRITKYP HHHHHHHHHHHHCCH	16.46	24719451
416	Phosphorylation	LLVTQRITKYPVLVE HHHHHHHHCCHHHHH	27.07	24719451
418	Phosphorylation	VTQRITKYPVLVERI HHHHHHCCHHHHHHH	7.02	24719451
477	Phosphorylation	AGKMDLKSSSKLKNG HCCCCCCCCHHHCCC	47.33	22210691
478	Phosphorylation	GKMDLKSSSKLKNGL CCCCCCCCHHHCCCC	29.88	22210691
479	Phosphorylation	KMDLKSSSKLKNGLT CCCCCCCHHHCCCCC	49.45	22210691
533	Acetylation	LLQEKDQKYVFASVD HHHHCCCCEEEEECC	54.22	7367749
534	Phosphorylation	LQEKDQKYVFASVDS HHHCCCCEEEEECCC	8.65	28348404
538	Phosphorylation	DQKYVFASVDSKPPV CCCEEEEECCCCCCE	18.26	28348404
541	Phosphorylation	YVFASVDSKPPVISL EEEEECCCCCCEEEE	44.93	27251275
542	Acetylation	VFASVDSKPPVISLQ EEEECCCCCCEEEEE	49.21	7367759
547	Phosphorylation	DSKPPVISLQKLIVR CCCCCEEEEEHHHHH	25.61	24719451
550	Acetylation	PPVISLQKLIVREVA CCEEEEEHHHHHHHC	45.53	7367769
583	Acetylation	YEIYTSSKEDRNAWM EEEECCCHHHHHHHH	64.50	30587007
635	Ubiquitination	FQERLSMKDQLIAQS HHHHHCHHHHHHHHH	38.66	-
642	Phosphorylation	KDQLIAQSLLEKQQI HHHHHHHHHHHHHHH	26.37	24719451
704	Phosphorylation	LICRQLGSANGQAED HHHHHHCCCCCCCCC	27.44	28122231
714	Phosphorylation	GQAEDGGSSTGPPRR CCCCCCCCCCCCCCC	30.20	28122231
715	Phosphorylation	QAEDGGSSTGPPRRA CCCCCCCCCCCCCCC	40.43	28122231
724	Phosphorylation	GPPRRAETFAGYDCT CCCCCCEECCCCCCC	20.14	27273156
728	Phosphorylation	RAETFAGYDCTNSPT CCEECCCCCCCCCCC	12.38	30266825
731	Phosphorylation	TFAGYDCTNSPTKNG ECCCCCCCCCCCCCC	35.51	30266825
733	Phosphorylation	AGYDCTNSPTKNGSF CCCCCCCCCCCCCCC	18.78	19664994
735	Phosphorylation	YDCTNSPTKNGSFKK CCCCCCCCCCCCCCC	36.64	23401153
739	Phosphorylation	NSPTKNGSFKKKVSS CCCCCCCCCCCCCCC	43.20	30108239
742	Ubiquitination	TKNGSFKKKVSSTDP CCCCCCCCCCCCCCC	57.29	-
745	Phosphorylation	GSFKKKVSSTDPRPR CCCCCCCCCCCCCCC	36.08	28348404
746	Phosphorylation	SFKKKVSSTDPRPRD CCCCCCCCCCCCCCC	39.56	25627689
747	Phosphorylation	FKKKVSSTDPRPRDW CCCCCCCCCCCCCCC	42.38	25159151
760	Phosphorylation	DWRGPPNSPDLKLSD CCCCCCCCCCCCCCC	26.09	23401153
766	Phosphorylation	NSPDLKLSDSDIPGS CCCCCCCCCCCCCCC	33.22	26657352
768	Phosphorylation	PDLKLSDSDIPGSSE CCCCCCCCCCCCCCC	33.78	26657352
773	Phosphorylation	SDSDIPGSSEESPQV CCCCCCCCCCCCCCE	29.26	27732954
774	Phosphorylation	DSDIPGSSEESPQVV CCCCCCCCCCCCCEE	51.83	29978859
777	Phosphorylation	IPGSSEESPQVVEAP CCCCCCCCCCEEECC	19.31	16964243
786	Phosphorylation	QVVEAPGTESDPRLP CEEECCCCCCCCCCC	31.12	29978859
788	Phosphorylation	VEAPGTESDPRLPTV EECCCCCCCCCCCHH	53.40	29978859
794	Phosphorylation	ESDPRLPTVLESELV CCCCCCCHHHHHHHH	42.17	-
806	Phosphorylation	ELVQRIQTLSQLLLN HHHHHHHHHHHHHHH	26.46	24043423
808	Phosphorylation	VQRIQTLSQLLLNLQ HHHHHHHHHHHHHHH	23.36	24043423
823	Phosphorylation	AVIAHQDSYVETQRA HHHHCCCHHHHHHHH	25.22	24043423
824	Phosphorylation	VIAHQDSYVETQRAA HHHCCCHHHHHHHHH	15.59	24043423
827	Phosphorylation	HQDSYVETQRAAIQE CCCHHHHHHHHHHHH	17.20	24043423
843	Phosphorylation	EKQFRLQSTRGNLLL HHHHHHHHHHHHHHH	25.35	26437602
844	Phosphorylation	KQFRLQSTRGNLLLE HHHHHHHHHHHHHHH	29.63	24719451
870	Acetylation	EERAALEKLQSQLRH HHHHHHHHHHHHHHH	53.31	23954790
912	Phosphorylation	GEARQLRERLEQERA HHHHHHHHHHHHHHH	70.93	-
921	Phosphorylation	LEQERAELERQRQAY HHHHHHHHHHHHHHH	6.84	-
960	Phosphorylation	RRLKKQNTAPGALPP HHHHHCCCCCCCCCC	31.56	26657352
1003	Phosphorylation	PSGVGPEYAERPEVA CCCCCHHHHCCHHHH	19.52	23917254
1014	Phosphorylation	PEVARRDSAPTENRL HHHHCCCCCCCCCCC	34.04	30177828
1049	Phosphorylation	AVQQQIPTKLAASTK HHHCCCCCHHHHHCC	40.08	28509920
1050	Acetylation	VQQQIPTKLAASTKG HHCCCCCHHHHHCCC	30.67	25953088
1064	Acetylation	GGKDKGGKSRGSQRW CCCCCCCCCCCCHHH	45.72	11791489
1065	Phosphorylation	GKDKGGKSRGSQRWE CCCCCCCCCCCHHHH	45.19	27251275
1068	Phosphorylation	KGGKSRGSQRWESSA CCCCCCCCHHHHCCC	18.57	27251275
1073	Phosphorylation	RGSQRWESSASFDLK CCCHHHHCCCCCCHH	25.01	23186163
1074	Phosphorylation	GSQRWESSASFDLKQ CCHHHHCCCCCCHHH	19.20	29802988
1076	Phosphorylation	QRWESSASFDLKQQL HHHHCCCCCCHHHHH	22.65	29507054
1080	Acetylation	SSASFDLKQQLLLNK CCCCCCHHHHHHHHH	37.53	11791499
1094	Phosphorylation	KLMGKDESTSRNRRS HHCCCCCCCCCCCCC	41.64	-
1095	Phosphorylation	LMGKDESTSRNRRSL HCCCCCCCCCCCCCC	29.65	-
1096	Phosphorylation	MGKDESTSRNRRSLS CCCCCCCCCCCCCCC	36.52	-
1101	Phosphorylation	STSRNRRSLSPILPG CCCCCCCCCCCCCCC	29.93	22167270
1103	Phosphorylation	SRNRRSLSPILPGRH CCCCCCCCCCCCCCC	15.90	19664994
1111	Phosphorylation	PILPGRHSPAPPPDP CCCCCCCCCCCCCCC	22.77	26055452
1124	Phosphorylation	DPGFPAPSPPPADSP CCCCCCCCCCCCCCC	52.74	25159151
1130	Phosphorylation	PSPPPADSPSEGFSL CCCCCCCCCCCCCCC	32.22	25159151
1132	Phosphorylation	PPPADSPSEGFSLKA CCCCCCCCCCCCCCC	55.06	30175587
1136	Phosphorylation	DSPSEGFSLKAGGTA CCCCCCCCCCCCCEE	39.56	28102081
1142	Phosphorylation	FSLKAGGTALLPGPP CCCCCCCEEECCCCC	17.14	26074081
1152	Phosphorylation	LPGPPAPSPLPATPL CCCCCCCCCCCCCCC	40.97	25159151
1157	Phosphorylation	APSPLPATPLSAKED CCCCCCCCCCCCCCC	23.87	25159151
1160	Phosphorylation	PLPATPLSAKEDASK CCCCCCCCCCCCCCC	38.20	28355574
1166	Phosphorylation	LSAKEDASKEDVIFF CCCCCCCCCCCCCCC	49.23	28450419
1289	Phosphorylation	--------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------		-
1291	Phosphorylation	----------------------------------------------------------------------------------------------------------------------------- -----------------------------------------------------------------------------------------------------------------------------		-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of ARHGI_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ARHGI_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ARHGI_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
GBB1_HUMAN	GNB1	physical	14512443

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ARHGI_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1103, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209; THR-212; SER-733;SER-1101; SER-1103; SER-1124 AND SER-1130, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1103, AND MASSSPECTROMETRY.	18220336 [Abstract]