dbPTM

ARHG8_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ARHG8_MOUSE
UniProt AC	Q9Z206
Protein Name	Neuroepithelial cell-transforming gene 1 protein
Gene Name	Net1
Organism	Mus musculus (Mouse).
Sequence Length	595
Subcellular Localization	Cytoplasm . Nucleus .
Protein Description	Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway. Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death..
Protein Sequence	MEPEPAAQKQPRPRRRSRRVSMLSEEPAAGLPADTPGPAANERCSLRRGSSFTFLTPGPHWDFTLKRKRREKDDDAVSLSSLDLKEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKSFSRSTVPTPTKRRSSALWSEMLDINMKESLTTREIKRQEAIYELSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDAYIPLHEDLLARIGEATKPDGTVEQIGHILVNWLPGLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILRHTPKDHRDVQLLEEAILIIQGVLSDINLKKGESECQYYINKLEYLDEKQKDPRIEASKVLLCHGELKNKSGHKLYIFLFQDILVLTRPVTRNERHLYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFGNSDKAKNIFRVRFQDPSPGHSHTLQANDVFHKQQWFNCIRAAIAPFQRAASPLELQGLPDLHEECEENNPSAGNLRAQRRSCVVPGVMQIDEESALDCGSSVQTVEDTRNMKAQRPQPGLRRARDKAQSGGKKKETLV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEPEPAAQ -------CCCCHHHC	20.48	-
21	Phosphorylation	RRRSRRVSMLSEEPA CCHHHHHHHCCCCCC	16.52	27566939
24	Phosphorylation	SRRVSMLSEEPAAGL HHHHHHCCCCCCCCC	31.13	26745281
50	Phosphorylation	RCSLRRGSSFTFLTP CCCCCCCCCEEECCC	22.07	26643407
51	Phosphorylation	CSLRRGSSFTFLTPG CCCCCCCCEEECCCC	31.00	26643407
53	Phosphorylation	LRRGSSFTFLTPGPH CCCCCCEEECCCCCC	21.49	26643407
80	Phosphorylation	DDDAVSLSSLDLKEP CCCCCCHHHCCCCCC	22.73	19854140
100	Phosphorylation	RPLARVTSLANLISP CHHHHHHHHHHHHHH	23.72	27841257
106	Phosphorylation	TSLANLISPVRNGAV HHHHHHHHHHHCCHH	22.10	26643407
122	Phosphorylation	RFGQTIQSFTLRGDH HHHHEEEEEEECCCC	19.15	-
146	Phosphorylation	FSRSTVPTPTKRRSS CCCCCCCCCCCCCCH	38.97	23384938
149	Acetylation	STVPTPTKRRSSALW CCCCCCCCCCCHHHH	47.20	6568429
152	Phosphorylation	PTPTKRRSSALWSEM CCCCCCCCHHHHHHH	24.81	22817900
153	Phosphorylation	TPTKRRSSALWSEML CCCCCCCHHHHHHHH	26.10	22817900
170	Phosphorylation	NMKESLTTREIKRQE CCCCCCCHHHHHHHH	31.43	20139300
368	Methylation	ECQYYINKLEYLDEK HHHHHHHHHHHHCCC	32.89	-
375	Methylation	KLEYLDEKQKDPRIE HHHHHCCCCCCCCHH	63.41	-
377	Methylation	EYLDEKQKDPRIEAS HHHCCCCCCCCHHHH	79.40	-
451	Phosphorylation	GDVRMGGSFRGAFGN CCCCCCCCCCCCCCC	13.17	-
454 (in isoform 2)	Phosphorylation	-	24.21	19144319
508	Phosphorylation	APFQRAASPLELQGL HHHHHCCCCHHHCCC	29.09	27087446
538	Phosphorylation	NLRAQRRSCVVPGVM HHHHHHHHCCCCCCE	17.29	22817900
583	Acetylation	GLRRARDKAQSGGKK CHHHHHHHHHHCCCC	42.65	19853563

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
152	S	Phosphorylation	Kinase	PAK1	Q13153	PSP
153	S	Phosphorylation	Kinase	PAK1	Q13153	PSP
538	S	Phosphorylation	Kinase	PAK1	Q13153	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ARHG8_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ARHG8_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
RHOA_HUMAN	RHOA	physical	9535835
CDC42_HUMAN	CDC42	physical	9535835

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ARHG8_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"The phagosomal proteome in interferon-gamma-activated macrophages."; Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.; Immunity 30:143-154(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-508, AND MASSSPECTROMETRY.	19144319 [Abstract]