dbPTM

ANR50_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ANR50_HUMAN
UniProt AC	Q9ULJ7
Protein Name	Ankyrin repeat domain-containing protein 50
Gene Name	ANKRD50
Organism	Homo sapiens (Human).
Sequence Length	1429
Subcellular Localization	Endosome .
Protein Description	Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1. [PubMed: 25278552]
Protein Sequence	MTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKSNCCNSAVNAPSLVMNSGNNASGVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPASLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSLSSKVQSLTIKSNSSGSTGGGDMQPSLRGLPNGPTHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHNLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHNSPSSEFEWSQVKPSLKSTKASKGGKSENSAKSGSAGKKAKQSNSSQPKVLEYEMTQFDRRGPIAKSGTAAPPKQMPAESQCKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MTNPWEEKV ------CCCHHHHHH	62.38	29255136
15	Phosphorylation	KVCKMAQTSLLQGKQ HHHHHHHHHHHCCCH	16.29	24719451
32	Malonylation	CREWVFHKLQHCLQE HHHHHHHHHHHHHHH	38.08	32601280
251	Ubiquitination	KMFTGFRKISLDDLR HHHHCCEECCHHHHH	34.09	-
251	Malonylation	KMFTGFRKISLDDLR HHHHCCEECCHHHHH	34.09	26320211
253	Phosphorylation	FTGFRKISLDDLRKA HHCCEECCHHHHHHH	28.87	25850435
288	Phosphorylation	RQHLTKETAEMLNQL HHHCCHHHHHHHHHH	29.49	23532336
365	Phosphorylation	LAACRPLTITELYHA HHHHCCCCHHHHHHH	28.65	24117733
367	Phosphorylation	ACRPLTITELYHAVW HHCCCCHHHHHHHHH	18.07	24117733
370	Phosphorylation	PLTITELYHAVWTKN CCCHHHHHHHHHCCC	4.98	24117733
375	Phosphorylation	ELYHAVWTKNMSLTL HHHHHHHCCCCCCCH	13.12	24117733
378	Sulfoxidation	HAVWTKNMSLTLEDF HHHHCCCCCCCHHHH	3.56	21406390
379	Phosphorylation	AVWTKNMSLTLEDFQ HHHCCCCCCCHHHHH	27.82	24117733
381	Phosphorylation	WTKNMSLTLEDFQRK HCCCCCCCHHHHHHH	22.31	46164357
388	Ubiquitination	TLEDFQRKLDILSKL CHHHHHHHHHHHHHH	39.46	-
388	Malonylation	TLEDFQRKLDILSKL CHHHHHHHHHHHHHH	39.46	26320211
393	Phosphorylation	QRKLDILSKLLVDGL HHHHHHHHHHHHCCC	22.91	21815630
514	Phosphorylation	VNSEDDRTSCIVRQA CCCCCHHHHHHHHHH	34.64	113334339
515	Phosphorylation	NSEDDRTSCIVRQAL CCCCHHHHHHHHHHH	11.62	28555341
527	Phosphorylation	QALEREDSIRTLLDN HHHHCCCHHHHHHHC	14.82	25849741
566	Phosphorylation	DVVNLLVSRGADLEI HHHHHHHHCCCCCEE	25.60	24719451
580	Phosphorylation	IEDAHGHTPLTLAAR EECCCCCCCEEHHHH	25.28	46164361
729	Phosphorylation	PASKGHASVVSLLID CCCCCCHHHHHHHEE	19.85	20873877
732	Phosphorylation	KGHASVVSLLIDRGA CCCHHHHHHHEECCC	18.46	20873877
942	Acetylation	HGADVNCKDADGRPT CCCCCCCCCCCCCCE	51.23	7308189
986	Phosphorylation	GRTALHVSCWQGHME CCCEEEEEEHHHHHH	10.01	19651622
1091	Phosphorylation	VAAKNGHSQIIKLLE HHHHHCHHHHHHHHH	25.19	46164341
1095	Ubiquitination	NGHSQIIKLLEKYGA HCHHHHHHHHHHHCC	48.96	-
1100	Phosphorylation	IIKLLEKYGASSLNG HHHHHHHHCCCCCCC	14.46	25002506
1103	Phosphorylation	LLEKYGASSLNGCSP HHHHHCCCCCCCCCC	30.87	29214152
1104	Phosphorylation	LEKYGASSLNGCSPS HHHHCCCCCCCCCCC	25.89	25002506
1109	Phosphorylation	ASSLNGCSPSPVHTM CCCCCCCCCCCCCCC	29.79	22199227
1111	Phosphorylation	SLNGCSPSPVHTMEQ CCCCCCCCCCCCCCC	24.22	25159151
1113	Phosphorylation	NGCSPSPVHTMEQKP CCCCCCCCCCCCCCC	7.43	18669648
1115	Phosphorylation	CSPSPVHTMEQKPLQ CCCCCCCCCCCCCHH	23.72	22199227
1116	Phosphorylation	SPSPVHTMEQKPLQS CCCCCCCCCCCCHHH	2.87	18669648
1119	Ubiquitination	PVHTMEQKPLQSLSS CCCCCCCCCHHHHHH	34.10	-
1123	Phosphorylation	MEQKPLQSLSSKVQS CCCCCHHHHHHHCEE	37.59	25002506
1127	Ubiquitination	PLQSLSSKVQSLTIK CHHHHHHHCEEEEEE	40.70	-
1130	Phosphorylation	SLSSKVQSLTIKSNS HHHHHCEEEEEECCC	30.39	24719451
1132	Phosphorylation	SSKVQSLTIKSNSSG HHHCEEEEEECCCCC	31.44	23909892
1135	Phosphorylation	VQSLTIKSNSSGSTG CEEEEEECCCCCCCC	37.79	22777824
1137	Phosphorylation	SLTIKSNSSGSTGGG EEEEECCCCCCCCCC	43.07	25849741
1138	Phosphorylation	LTIKSNSSGSTGGGD EEEECCCCCCCCCCC	40.80	25159151
1140	Phosphorylation	IKSNSSGSTGGGDMQ EECCCCCCCCCCCCC	26.31	25159151
1141	Phosphorylation	KSNSSGSTGGGDMQP ECCCCCCCCCCCCCC	43.29	28985074
1146	Sulfoxidation	GSTGGGDMQPSLRGL CCCCCCCCCCHHCCC	7.74	21406390
1149	Phosphorylation	GGGDMQPSLRGLPNG CCCCCCCHHCCCCCC	17.51	30177828
1158	Phosphorylation	RGLPNGPTHAFSSPS CCCCCCCCCCCCCCC	27.51	25159151
1162	Phosphorylation	NGPTHAFSSPSESPD CCCCCCCCCCCCCCC	41.97	30266825
1163	Phosphorylation	GPTHAFSSPSESPDS CCCCCCCCCCCCCCC	26.45	30266825
1165	Phosphorylation	THAFSSPSESPDSTV CCCCCCCCCCCCCHH	53.29	30266825
1167	Phosphorylation	AFSSPSESPDSTVDR CCCCCCCCCCCHHHH	38.04	30266825
1168	Phosphorylation	FSSPSESPDSTVDRQ CCCCCCCCCCHHHHH	35.20	18220336
1170	Phosphorylation	SPSESPDSTVDRQKS CCCCCCCCHHHHHHH	33.44	30266825
1171	Phosphorylation	PSESPDSTVDRQKSS CCCCCCCHHHHHHHH	33.53	30266825
1177	Phosphorylation	STVDRQKSSLSNNSL CHHHHHHHHCCCCCC	28.33	27732954
1178	Phosphorylation	TVDRQKSSLSNNSLK HHHHHHHHCCCCCCC	42.74	23403867
1180	Phosphorylation	DRQKSSLSNNSLKSS HHHHHHCCCCCCCCC	35.59	25159151
1183	Phosphorylation	KSSLSNNSLKSSKNS HHHCCCCCCCCCCCC	41.23	25159151
1186	Phosphorylation	LSNNSLKSSKNSSLR CCCCCCCCCCCCCCC	52.41	25849741
1187	Phosphorylation	SNNSLKSSKNSSLRT CCCCCCCCCCCCCCC	33.98	27732954
1194	Phosphorylation	SKNSSLRTTSSTATA CCCCCCCCCCCCCCC	36.00	23927012
1195	Phosphorylation	KNSSLRTTSSTATAQ CCCCCCCCCCCCCCE	17.78	23927012
1196	Phosphorylation	NSSLRTTSSTATAQT CCCCCCCCCCCCCEE	25.69	23927012
1197	Phosphorylation	SSLRTTSSTATAQTV CCCCCCCCCCCCEEE	21.70	23927012
1198	Phosphorylation	SLRTTSSTATAQTVP CCCCCCCCCCCEEEC	28.00	23927012
1200	Phosphorylation	RTTSSTATAQTVPID CCCCCCCCCEEECCC	21.27	23927012
1203	Phosphorylation	SSTATAQTVPIDSFH CCCCCCEEECCCCCC	26.28	28348404
1208	Phosphorylation	AQTVPIDSFHNLSFT CEEECCCCCCCCCHH	29.17	63711767
1213	Phosphorylation	IDSFHNLSFTEQIQQ CCCCCCCCHHHHHHH	35.12	25159151
1215	Phosphorylation	SFHNLSFTEQIQQHS CCCCCCHHHHHHHCC	24.49	30576142
1222	Phosphorylation	TEQIQQHSLPRSRSR HHHHHHCCCCCCCCC	35.41	23927012
1226	Phosphorylation	QQHSLPRSRSRQSIV HHCCCCCCCCCCCCC	32.44	29449344
1228	Phosphorylation	HSLPRSRSRQSIVSP CCCCCCCCCCCCCCC	35.60	25849741
1231	Phosphorylation	PRSRSRQSIVSPSST CCCCCCCCCCCCCHH	24.69	25849741
1234	Phosphorylation	RSRQSIVSPSSTTQS CCCCCCCCCCHHHHC	19.86	25159151
1236	Phosphorylation	RQSIVSPSSTTQSLG CCCCCCCCHHHHCCC	32.58	25627689
1237	Phosphorylation	QSIVSPSSTTQSLGQ CCCCCCCHHHHCCCC	38.57	28348404
1238	Phosphorylation	SIVSPSSTTQSLGQS CCCCCCHHHHCCCCC	32.94	28348404
1239	Phosphorylation	IVSPSSTTQSLGQSH CCCCCHHHHCCCCCC	20.36	22210691
1241	Phosphorylation	SPSSTTQSLGQSHNS CCCHHHHCCCCCCCC	32.16	28348404
1245	Phosphorylation	TTQSLGQSHNSPSSE HHHCCCCCCCCCCCC	23.88	25159151
1248	Phosphorylation	SLGQSHNSPSSEFEW CCCCCCCCCCCCCCH	22.27	25849741
1250	Phosphorylation	GQSHNSPSSEFEWSQ CCCCCCCCCCCCHHH	42.17	28348404
1251	Phosphorylation	QSHNSPSSEFEWSQV CCCCCCCCCCCHHHC	50.08	28348404
1256	Phosphorylation	PSSEFEWSQVKPSLK CCCCCCHHHCCHHHH	20.21	29449344
1261	Phosphorylation	EWSQVKPSLKSTKAS CHHHCCHHHHCCCCC	42.59	81442427
1269	Trimethylation	LKSTKASKGGKSENS HHCCCCCCCCCCCCC	76.24	-
1269	Methylation	LKSTKASKGGKSENS HHCCCCCCCCCCCCC	76.24	-
1273	Phosphorylation	KASKGGKSENSAKSG CCCCCCCCCCCCCCC	45.33	68720971
1276	Phosphorylation	KGGKSENSAKSGSAG CCCCCCCCCCCCCHH	32.78	68720977
1278	Trimethylation	GKSENSAKSGSAGKK CCCCCCCCCCCHHHH	55.99	-
1278	Methylation	GKSENSAKSGSAGKK CCCCCCCCCCCHHHH	55.99	-
1279	Phosphorylation	KSENSAKSGSAGKKA CCCCCCCCCCHHHHH	37.38	68720983
1281	Phosphorylation	ENSAKSGSAGKKAKQ CCCCCCCCHHHHHCC	40.72	24365823
1284	Trimethylation	AKSGSAGKKAKQSNS CCCCCHHHHHCCCCC	49.64	-
1284	Methylation	AKSGSAGKKAKQSNS CCCCCHHHHHCCCCC	49.64	-
1289	Phosphorylation	AGKKAKQSNSSQPKV HHHHHCCCCCCCCCE	37.66	30576142
1292	Phosphorylation	KAKQSNSSQPKVLEY HHCCCCCCCCCEEEE	55.54	30576142
1299	Phosphorylation	SQPKVLEYEMTQFDR CCCCEEEEEECCCCC	13.57	21945579
1302	Phosphorylation	KVLEYEMTQFDRRGP CEEEEEECCCCCCCC	17.72	27251275
1352	Phosphorylation	QFLIHQQSGEQKKRN HHHHHHCCCCHHHHC	37.41	29457462
1367	Phosphorylation	GIMTNPNYHLQSNQV CCCCCCCCCCCCCCE	12.74	30835499
1397	Phosphorylation	HQEVLEGYPSSETEL CHHHHCCCCCCHHHC	7.20	23186163
1399	Phosphorylation	EVLEGYPSSETELSL HHHCCCCCCHHHCCH	32.11	23403867
1400	Phosphorylation	VLEGYPSSETELSLK HHCCCCCCHHHCCHH	43.99	25849741
1402	Phosphorylation	EGYPSSETELSLKQA CCCCCCHHHCCHHHH	44.14	23403867
1405	Phosphorylation	PSSETELSLKQALKL CCCHHHCCHHHHHHH	27.56	23403867
1417	Phosphorylation	LKLQIEGSDPSFNYK HHHHCCCCCCCCCCC	33.29	113334331

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	SMURF1	Q9HCE7	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ANR50_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ANR50_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
A4_HUMAN	APP	physical	21832049

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ANR50_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1222, AND MASSSPECTROMETRY.	18220336 [Abstract]