ACTB_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ACTB_RAT
• UniProt AC: P60711
• Protein Name: Actin, cytoplasmic 1
• Gene Name: Actb
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 375
• Subcellular Localization: Cytoplasm, cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs..
• Protein Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells..
• Protein Sequence: MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MDDDIAAL -------CCCCCEEE	13.23	-
2	Acetylation	------MDDDIAALV ------CCCCCEEEE	63.39	-
14	Phosphorylation	ALVVDNGSGMCKAGF EEEEECCCCCEECCC	29.61	23984901
33	Phosphorylation	APRAVFPSIVGRPRH CCCCCCHHHCCCCCC	20.59	5130775
44	Oxidation	RPRHQGVMVGMGQKD CCCCCCEEEECCCCC	2.49	-
47	Oxidation	HQGVMVGMGQKDSYV CCCEEEECCCCCCCC	3.32	-
50	Ubiquitination	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	-
50	Acetylation	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	22902405
52	Phosphorylation	VGMGQKDSYVGDEAQ EECCCCCCCCCCHHH	28.84	25403869
53	Phosphorylation	GMGQKDSYVGDEAQS ECCCCCCCCCCHHHC	20.70	4817623
60	Phosphorylation	YVGDEAQSKRGILTL CCCCHHHCCCCEEEE	31.22	29779826
61	Ubiquitination	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	-
61	Acetylation	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	25786129
66	Phosphorylation	QSKRGILTLKYPIEH HCCCCEEEEECEECC	21.20	28826663
68	Acetylation	KRGILTLKYPIEHGI CCCEEEEECEECCCC	43.69	22902405
73	Methylation	TLKYPIEHGIVTNWD EEECEECCCCCCCHH	31.81	30526847
84	Methylation	TNWDDMEKIWHHTFY CCHHHHHHHHHHHHH	44.45	-
84	Acetylation	TNWDDMEKIWHHTFY CCHHHHHHHHHHHHH	44.45	22902405
89	Phosphorylation	MEKIWHHTFYNELRV HHHHHHHHHHCCCCC	18.22	9548383
91	Phosphorylation	KIWHHTFYNELRVAP HHHHHHHHCCCCCCC	14.64	174691
113	Acetylation	TEAPLNPKANREKMT EECCCCCCCCHHHHH	58.16	22902405
113	Ubiquitination	TEAPLNPKANREKMT EECCCCCCCCHHHHH	58.16	-
166	Phosphorylation	VTHTVPIYEGYALPH CEEEEEEECCCCCCH	9.38	174693
169	Phosphorylation	TVPIYEGYALPHAIL EEEEECCCCCCHHHH	7.94	174695
186	Phosphorylation	DLAGRDLTDYLMKIL HHCCCCHHHHHHHHH	27.21	22673903
188	Phosphorylation	AGRDLTDYLMKILTE CCCCHHHHHHHHHHH	11.94	18876225
191	Ubiquitination	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	-
191	Acetylation	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	25786129
198	Phosphorylation	KILTERGYSFTTTAE HHHHHCCCCCCCCHH	13.56	174687
199	Phosphorylation	ILTERGYSFTTTAER HHHHCCCCCCCCHHH	20.95	27097102
199	O-linked_Glycosylation	ILTERGYSFTTTAER HHHHCCCCCCCCHHH	20.95	21933451
201	Phosphorylation	TERGYSFTTTAEREI HHCCCCCCCCHHHHH	19.80	27097102
202	Phosphorylation	ERGYSFTTTAEREIV HCCCCCCCCHHHHHH	23.23	27097102
203	Phosphorylation	RGYSFTTTAEREIVR CCCCCCCCHHHHHHH	24.38	27097102
213	Acetylation	REIVRDIKEKLCYVA HHHHHHHHHHHHEEE	53.75	22902405
215	Acetylation	IVRDIKEKLCYVALD HHHHHHHHHHEEECC	38.56	25786129
215	Ubiquitination	IVRDIKEKLCYVALD HHHHHHHHHHEEECC	38.56	-
218	Phosphorylation	DIKEKLCYVALDFEQ HHHHHHHEEECCHHH	10.13	25532521
229	Phosphorylation	DFEQEMATAASSSSL CHHHHHHHHHCCCCC	22.80	28551015
232	Phosphorylation	QEMATAASSSSLEKS HHHHHHHCCCCCCCC	28.43	27097102
233	Phosphorylation	EMATAASSSSLEKSY HHHHHHCCCCCCCCE	21.15	27097102
234	Phosphorylation	MATAASSSSLEKSYE HHHHHCCCCCCCCEE	36.13	27097102
235	Phosphorylation	ATAASSSSLEKSYEL HHHHCCCCCCCCEEC	42.36	27097102
239	Phosphorylation	SSSSLEKSYELPDGQ CCCCCCCCEECCCCC	17.92	28689409
240	Phosphorylation	SSSLEKSYELPDGQV CCCCCCCEECCCCCE	32.33	22108457
249	Phosphorylation	LPDGQVITIGNERFR CCCCCEEEECCCEEC	24.79	46314633
291	Ubiquitination	KCDVDIRKDLYANTV CCCCCCCCHHHCCCE	53.04	-
291	Acetylation	KCDVDIRKDLYANTV CCCCCCCCHHHCCCE	53.04	16212439
294	Phosphorylation	VDIRKDLYANTVLSG CCCCCHHHCCCEECC	14.13	174707
297	Phosphorylation	RKDLYANTVLSGGTT CCHHHCCCEECCCCC	18.04	25532521
300	Phosphorylation	LYANTVLSGGTTMYP HHCCCEECCCCCCCC	30.73	22108457
304	Phosphorylation	TVLSGGTTMYPGIAD CEECCCCCCCCCHHH	20.27	22108457
306	Phosphorylation	LSGGTTMYPGIADRM ECCCCCCCCCHHHHH	9.01	-
315	Acetylation	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	22902405
315	Ubiquitination	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	-
318	Phosphorylation	DRMQKEITALAPSTM HHHHHHHHHHCCCCE	19.40	3973057
323	Phosphorylation	EITALAPSTMKIKII HHHHHCCCCEEEEEE	35.21	9423901
324	Phosphorylation	ITALAPSTMKIKIIA HHHHCCCCEEEEEEC	22.79	27097102
326	Ubiquitination	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	-
326	Acetylation	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	25786129
328	Ubiquitination	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	-
328	Acetylation	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	25786129
338	Phosphorylation	APPERKYSVWIGGSI CCCCCCEEEEECHHH	17.63	30417516
362	Phosphorylation	MWISKQEYDESGPSI HHHHCCCCCCCCCCC	23.63	2081829
365	Phosphorylation	SKQEYDESGPSIVHR HCCCCCCCCCCCCCC	53.78	29764405
368	Phosphorylation	EYDESGPSIVHRKCF CCCCCCCCCCCCCCC	40.54	22673903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
33	S	Phosphorylation	Kinase	PRKACA	P00517	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
44	M	Oxidation	Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.	-
47	M	Oxidation	Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.	-
73	H	Methylation	Methylated at His-73 by SETD3.	30526847
73	H	Methylation	Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).	30526847
84	K	Methylation	Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ACTB_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ESPN_RAT	Espn	physical	12598619

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.